Restriction map for
pF1KE0091
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# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII AclI AflII AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I AspEI AviII
AvrII BamHI BbrPI BciVI BfrI
BglI BlnI BsaI BsgI BsiEI
BspDI BspMI BstAPI BstBI Bsu36I
ClaI CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRV EcoT22I FseI FspI KspI
MfeI MluI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 1092 GGTACC G^GTACC
AccIII 1289 TCCGGA T^CCGGA
AcsI 299 GAATTC [GA]^AATT[CT]
AgeI 1481 ACCGGT A^CCGGT
ApoI 299 GAATTC [GA]^AATT[CT]
Asp718I 1092 GGTACC G^GTACC
AspI 738 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 1806 GAGCCC G[GA]GC[CT]^C
BfaI 1321 CTAG C^TAG
BlpI 180 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 180 GCTGAGC GC^T[ACGT]AGC
BseAI 1289 TCCGGA T^CCGGA
BsiWI 1079 CGTACG C^GTACG
BsmBI 1885 GAGACG CGTCTC(1/5)
Bsp1407I 1379 TGTACA T^GTACA
BspEI 1289 TCCGGA T^CCGGA
BspLU11I 1271 ACATGT A^CATGT
BsrGI 1379 TGTACA T^GTACA
BssHII 106 GCGCGC G^CGCGC
Bst1107I 760 GTATAC GTA^TAC
BstZ17I 760 GTATAC GTA^TAC
CelII 180 GCTGAGC GC^T[ACGT]AGC
DraIII 1713 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 1429 CTCTTC CTCTTC(1/4)
EcoRI 299 GAATTC G^AATTC
HindIII 1795 AAGCTT A^AGCTT
HpaI 1362 GTTAAC GTT^AAC
KpnI 1092 GGTACC GGTAC^C
Ksp632I 1429 CTCTTC CTCTTC(1/4)
MaeI 1321 CTAG C^TAG
MroI 1289 TCCGGA T^CCGGA
PflFI 738 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1481 ACCGGT A^CCGGT
PpuMI 629 GGGTCCT [GA]G^G[AT]CC[CT]
SmaI 826 CCCGGG CCC^GGG
SspBI 1379 TGTACA T^GTACA
TaqI 653 TCGA T^CGA
TfiI 267 GATTC G^A[AT]TC
Tth111I 738 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 653 TCGA T^CGA
XcmI 1097 CCATTGCTGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 826 CCCGGG C^CCGGG
XmaI 826 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 43 enzymes) #
###################################
AccI 744 GTCTAC GT^[AC][GT]AC
760 GTATAC
AcyI 66 GGCGCC G[GA]^CG[CT]C
99 GGCGCC
AlwNI 550 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
993 CAGGGACTG
AspHI 622 GAGCAC G[AT]GC[AT]^C
655 GAGCAC
AvaI 826 CCCGGG C^[CT]CG[GA]G
1741 CCCGAG
BalI 635 TGGCCA TGG^CCA
1436 TGGCCA
BbeI 66 GGCGCC GGCGC^C
99 GGCGCC
BbsI 1501 GAAGAC GAAGAC(2/6)
1722 GAAGAC
BbvCI 43 GCTGAGG CCTCAGC(-5/-2)
1102 GCTGAGG
BcgI 1028 GCACCAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1451 GCATCTGCATCG
BclI 1550 TGATCA T^GATCA
1595 TGATCA
BglII 914 AGATCT A^GATCT
1776 AGATCT
BpmI 1750 CTCCAG CTGGAG(16/14)
316 CTGGAG
BpuAI 1501 GAAGAC GAAGAC(2/6)
1722 GAAGAC
BsaHI 66 GGCGCC G[GA]^CG[CT]C
99 GGCGCC
BsaWI 1289 TCCGGA [AT]^CCGG[AT]
1481 ACCGGT
BseRI 46 GAGGAG GAGGAG(10/8)
251 GAGGAG
BsiHKAI 622 GAGCAC G[AT]GC[AT]^C
655 GAGCAC
BsmI 591 GAATGC GAATGC(1/-1)
259 GCATTC
BsoBI 826 CCCGGG C^[CT]CG[GA]G
1741 CCCGAG
BspHI 1199 TCATGA T^CATGA
1553 TCATGA
BsrBI 953 CCGCTC CCGCTC(-3/-3)
1696 CCGCTC
BsrDI 1098 CATTGC GCAATG(2/0)
29 GCAATG
BssSI 142 CACGAG CACGAG(-5/-1)
1354 CTCGTG
BstXI 932 CCATTTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1031 CCAGCTTCGTGG
DrdI 130 GACAAGGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
883 GACCCCCAGGTC
DsaI 1262 CCGTGG C^C[GA][CT]GG
1810 CCACGG
EcoT14I 1148 CCAAGG C^C[AT][AT]GG
1280 CCAAGG
FbaI 1550 TGATCA T^GATCA
1595 TGATCA
HgaI 1887 GACGC GACGC(5/10)
119 GCGTC
Hin1I 66 GGCGCC G[GA]^CG[CT]C
99 GGCGCC
HincII 1362 GTTAAC GT[CT]^[GA]AC
1758 GTTGAC
HindII 1362 GTTAAC GT[CT]^[GA]AC
1758 GTTGAC
KasI 66 GGCGCC G^GCGCC
99 GGCGCC
MluNI 635 TGGCCA TGG^CCA
1436 TGGCCA
MscI 635 TGGCCA TGG^CCA
1436 TGGCCA
NarI 66 GGCGCC GG^CGCC
99 GGCGCC
NspI 1271 ACATGT [GA]CATG^[CT]
1376 GCATGT
PstI 81 CTGCAG CTGCA^G
586 CTGCAG
RcaI 1199 TCATGA T^CATGA
1553 TCATGA
SfoI 66 GGCGCC GGC^GCC
99 GGCGCC
SmlI 1612 CTCAAG C^T[CT][GA]AG
1846 CTCAAG
StyI 1148 CCAAGG C^C[AT][AT]GG
1280 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AvaII BanI BbvI BcnI
BmyI BsaAI BsaBI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrFI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoO65I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MamI MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII