Restriction map for
pF1KB8440
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I AspEI AspI AvaI
AvaII AviII AvrII BalI BamHI
BbvCI BcgI BclI BfaI BfrI
BglI BlnI BlpI Bpu1102I BsaBI
BsaWI BseAI BseRI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BsrBI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DdeI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HgaI HincII HindII HindIII HpaI
KspI MaeI MamI MfeI MluI
MluNI MroI MscI MunI NcoI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PleI
PmeI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tsp45I Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 206 GGTACC G^GTACC
AcsI 234 GAATTC [GA]^AATT[CT]
AcyI 351 GGCGCC G[GA]^CG[CT]C
ApoI 234 GAATTC [GA]^AATT[CT]
Asp718I 206 GGTACC G^GTACC
BanII 1237 GAGCTC G[GA]GC[CT]^C
BbeI 351 GGCGCC GGCGC^C
BbsI 1191 GAAGAC GAAGAC(2/6)
BciVI 681 GTATCC GTATCC(6/5)
BglII 182 AGATCT A^GATCT
BpuAI 1191 GAAGAC GAAGAC(2/6)
BsaHI 351 GGCGCC G[GA]^CG[CT]C
BsaI 1228 GAGACC GGTCTC(1/5)
BsgI 143 CTGCAC GTGCAG(16/14)
BsiEI 922 CGACCG CG[GA][CT]^CG
BspHI 390 TCATGA T^CATGA
BspLU11I 214 ACATGT A^CATGT
BspMI 703 ACCTGC ACCTGC(4/8)
BsrDI 426 CATTGC GCAATG(2/0)
BssSI 433 CTCGTG CACGAG(-5/-1)
BstF5I 167 GGATG GGATG(2/0)
DraII 154 GGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 110 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 47 CCGTGG C^C[GA][CT]GG
EarI 556 GAAGAG CTCTTC(1/4)
EcoO109I 154 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 234 GAATTC G^AATTC
FokI 167 GGATG GGATG(9/13)
Hin1I 351 GGCGCC G[GA]^CG[CT]C
KasI 351 GGCGCC G^GCGCC
KpnI 206 GGTACC GGTAC^C
Ksp632I 556 GAAGAG CTCTTC(1/4)
MseI 497 TTAA T^TAA
MslI 211 CACACATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 717 CAGCGG C[AC]G^C[GT]G
NaeI 538 GCCGGC GCC^GGC
NarI 351 GGCGCC GG^CGCC
NdeI 326 CATATG CA^TATG
NgoMIV 538 GCCGGC G^CCGGC
NspI 214 ACATGT [GA]CATG^[CT]
PflMI 109 CCACAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 390 TCATGA T^CATGA
SacI 1237 GAGCTC GAGCT^C
SfoI 351 GGCGCC GGC^GCC
StuI 504 AGGCCT AGG^CCT
Tru9I 497 TTAA T^TAA
Van91I 109 CCACAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 636 CCAGTGGTACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 652 CGCG CG^CG
675 CGCG
AlwI 603 GGATC GGATC(4/5)
851 GGATC
AspHI 247 GTGCTC G[AT]GC[AT]^C
1237 GAGCTC
BanI 206 GGTACC G^G[CT][GA]CC
351 GGCGCC
BbrPI 69 CACGTG CAC^GTG
596 CACGTG
BcnI 478 CCGGG CC^[GC]GG
814 CCCGG
BsiHKAI 247 GTGCTC G[AT]GC[AT]^C
1237 GAGCTC
BsrFI 538 GCCGGC [GA]^CCGG[CT]
1017 ACCGGC
BstUI 652 CGCG CG^CG
675 CGCG
BstXI 23 CCATTCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
210 CCACACATGTGG
BstYI 182 AGATCT [GA]^GATC[CT]
603 GGATCT
Cfr10I 538 GCCGGC [GA]^CCGG[CT]
1017 ACCGGC
EcoT14I 973 CCTTGG C^C[AT][AT]GG
1137 CCAAGG
MaeIII 90 GTTAC ^GT[ACGT]AC
626 GTAAC
MflI 182 AGATCT [GA]^GATC[CT]
603 GGATCT
MvnI 652 CGCG CG^CG
675 CGCG
NciI 478 CCGGG CC^[GC]GG
814 CCCGG
PmaCI 69 CACGTG CAC^GTG
596 CACGTG
PmlI 69 CACGTG CAC^GTG
596 CACGTG
SfaNI 9 GATGC GCATC(5/9)
125 GCATC
SfcI 686 CTGTAG C^T[GA][CT]AG
1162 CTACAG
StyI 973 CCTTGG C^C[AT][AT]GG
1137 CCAAGG
Tsp509I 235 AATT ^AATT
321 AATT
XhoII 182 AGATCT [GA]^GATC[CT]
603 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AfaI AflIII AluI BbvI
BmyI BpmI BsaAI BsaJI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstNI Cac8I CfoI Cfr13I DpnII
EaeI EcoRII FauI Fnu4HI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MboI
MboII MnlI MspI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI TaqI TfiI TseI
TspRI TthHB8I