Restriction map for
pF1KB3608
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BanII BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BlnI BmyI BsaAI BsaBI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsmI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrGI BsrI BssHII Bst1107I BstAPI
BstBI BstF5I BstZ17I Bsu36I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoRI EcoRV
EcoT22I FbaI FokI FseI FspI
HincII HindII HindIII HpaI KpnI
Ksp632I KspI MaeI MamI MfeI
MluI MluNI MroI MscI MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfcI SfiI SfuI
SgrAI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TfiI
Tth111I Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 203 CGCG CG^CG
AcsI 580 AAATTC [GA]^AATT[CT]
AcyI 230 GGCGCC G[GA]^CG[CT]C
AlwI 18 GATCC GGATC(4/5)
Aor51HI 290 AGCGCT AGC^GCT
ApoI 580 AAATTC [GA]^AATT[CT]
AvaII 87 GGACC G^G[AT]CC
BanI 230 GGCGCC G^G[CT][GA]CC
BbeI 230 GGCGCC GGCGC^C
BbsI 424 GAAGAC GAAGAC(2/6)
BglII 221 AGATCT A^GATCT
BlpI 172 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 172 GCTGAGC GC^T[ACGT]AGC
BpuAI 424 GAAGAC GAAGAC(2/6)
BsaHI 230 GGCGCC G[GA]^CG[CT]C
BsaWI 575 ACCGGA [AT]^CCGG[AT]
BsrDI 168 CATTGC GCAATG(2/0)
BssSI 137 CTCGTG CACGAG(-5/-1)
BstEII 528 GGTGACC G^GT[ACGT]ACC
BstPI 528 GGTGACC G^GT[ACGT]ACC
BstUI 203 CGCG CG^CG
BstXI 254 CCAACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 221 AGATCT [GA]^GATC[CT]
CelII 172 GCTGAGC GC^T[ACGT]AGC
DsaI 410 CCACGG C^C[GA][CT]GG
Eco47III 290 AGCGCT AGC^GCT
EcoO65I 528 GGTGACC G^GT[ACGT]ACC
FauI 101 CCCGC CCCGC(4/6)
Hin1I 230 GGCGCC G[GA]^CG[CT]C
HinfI 548 GAGTC G^A[ACGT]TC
KasI 230 GGCGCC G^GCGCC
MaeII 320 ACGT A^CGT
MflI 221 AGATCT [GA]^GATC[CT]
MseI 401 TTAA T^TAA
MvnI 203 CGCG CG^CG
NarI 230 GGCGCC GG^CGCC
NspI 436 GCATGC [GA]CATG^[CT]
PleI 548 GAGTC GAGTC(4/5)
SfoI 230 GGCGCC GGC^GCC
SmaI 483 CCCGGG CCC^GGG
SphI 436 GCATGC GCATG^C
Tru9I 401 TTAA T^TAA
Tsp509I 581 AATT ^AATT
XcmI 410 CCACGGTGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 221 AGATCT [GA]^GATC[CT]
XmaCI 483 CCCGGG C^CCGGG
XmaI 483 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AvaI 483 CCCGGG C^[CT]CG[GA]G
524 CTCGGG
BpmI 113 CTGGAG CTGGAG(16/14)
124 CTGGAG
BsaI 56 GAGACC GGTCTC(1/5)
616 GAGACC
BsoBI 483 CCCGGG C^[CT]CG[GA]G
524 CTCGGG
BsrBI 21 CCGCTC CCGCTC(-3/-3)
102 CCGCTC
BsrFI 177 GCCGGC [GA]^CCGG[CT]
554 GCCGGC
Cfr10I 177 GCCGGC [GA]^CCGG[CT]
554 GCCGGC
DdeI 4 CTGAG C^T[ACGT]AG
173 CTGAG
EaeI 179 CGGCCA [CT]^GGCC[GA]
268 TGGCCG
EcoT14I 563 CCAAGG C^C[AT][AT]GG
621 CCTTGG
HaeII 230 GGCGCC [GA]GCGC^[CT]
290 AGCGCT
HgaI 201 GACGC GACGC(5/10)
417 GACGC
MspA1I 346 CAGCTG C[AC]G^C[GT]G
458 CAGCTG
NaeI 177 GCCGGC GCC^GGC
554 GCCGGC
NgoMIV 177 GCCGGC G^CCGGC
554 GCCGGC
PvuII 346 CAGCTG CAG^CTG
458 CAGCTG
StyI 563 CCAAGG C^C[AT][AT]GG
621 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AluI BbvI BcnI BsaJI
BsgI BsiYI BslI BsmAI BssKI
BstNI Cac8I CfoI Cfr13I DpnII
EcoRII Fnu4HI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeIII
MboI MboII MnlI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI TaqI
TseI Tsp45I TspRI TthHB8I