Restriction map for
pF1KB5561
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# NO CUT ENZYMES ( 149 enzymes) #
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AatII Acc65I AccII AciI AclI
AflII AgeI AhdI AlwNI AocI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BcnI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsgI BsiEI
BsiWI BsmBI BsmI BsoBI BspDI
BspHI BspMI BsrBI BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstF5I
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FokI FseI FspI HaeII HhaI
HinP1I HpaI KasI KpnI KspI
MaeII MamI MfeI MluI MspA1I
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tth111I Van91I XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccIII 681 TCCGGA T^CCGGA
AcyI 669 GACGCC G[GA]^CG[CT]C
Alw44I 143 GTGCAC G^TGCAC
ApaI 986 GGGCCC GGGCC^C
ApaLI 143 GTGCAC G^TGCAC
AvaII 426 GGACC G^G[AT]CC
BalI 189 TGGCCA TGG^CCA
BanII 986 GGGCCC G[GA]GC[CT]^C
BbsI 1192 GAAGAC GAAGAC(2/6)
BbvI 1235 GCAGC GCAGC(8/12)
BclI 417 TGATCA T^GATCA
BpuAI 1192 GAAGAC GAAGAC(2/6)
BsaHI 669 GACGCC G[GA]^CG[CT]C
BsaI 91 GAGACC GGTCTC(1/5)
BsaWI 681 TCCGGA [AT]^CCGG[AT]
BseAI 681 TCCGGA T^CCGGA
BseRI 6 GAGGAG GAGGAG(10/8)
BsmFI 667 GGGAC GGGAC(10/14)
Bsp1407I 432 TGTACA T^GTACA
BspEI 681 TCCGGA T^CCGGA
BsrGI 432 TGTACA T^GTACA
BstAPI 932 GCACTTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DsaI 1371 CCATGG C^C[GA][CT]GG
EaeI 189 TGGCCA [CT]^GGCC[GA]
EcoT14I 1371 CCATGG C^C[AT][AT]GG
FbaI 417 TGATCA T^GATCA
Fnu4HI 1235 GCAGC GC^[ACGT]GC
HapII 682 CCGG C^CGG
HgaI 669 GACGC GACGC(5/10)
Hin1I 669 GACGCC G[GA]^CG[CT]C
HincII 1346 GTCGAC GT[CT]^[GA]AC
HindII 1346 GTCGAC GT[CT]^[GA]AC
HpaII 682 CCGG C^CGG
ItaI 1235 GCAGC GC^[ACGT]GC
MluNI 189 TGGCCA TGG^CCA
MroI 681 TCCGGA T^CCGGA
MscI 189 TGGCCA TGG^CCA
MslI 709 CATTCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 682 CCGG C^CGG
NcoI 1371 CCATGG C^CATGG
NlaIV 986 GGGCCC GG[ACGT]^[ACGT]CC
PpuMI 425 AGGACCT [GA]G^G[AT]CC[CT]
SalI 1346 GTCGAC G^TCGAC
SfaNI 691 GATGC GCATC(5/9)
SspBI 432 TGTACA T^GTACA
StyI 1371 CCATGG C^C[AT][AT]GG
TseI 1235 GCAGC G^C[AT]GC
Tsp45I 398 GTCAC ^GT[GC]AC
TspRI 654 CAGTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccI 1346 GTCGAC GT^[AC][GT]AC
1451 GTAGAC
AflIII 32 ACATGT A^C[GA][CT]GT
1405 ACATGT
AspHI 143 GTGCAC G[AT]GC[AT]^C
301 GAGCAC
BsaJI 95 CCCTGG C^C[ACGT][ACGT]GG
1371 CCATGG
BsiHKAI 143 GTGCAC G[AT]GC[AT]^C
301 GAGCAC
BsiYI 43 CCTACTGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1351 CCTAAAAATGG
BslI 43 CCTACTGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1351 CCTAAAAATGG
BsmAI 91 GAGAC GTCTC(1/5)
517 GAGAC
BspLU11I 32 ACATGT A^CATGT
1405 ACATGT
BssKI 96 CCTGG ^CC[ACGT]GG
606 CCTGG
BstNI 96 CCTGG CC^[AT]GG
606 CCTGG
BstYI 684 GGATCT [GA]^GATC[CT]
897 AGATCC
DraII 425 AGGACCT [GA]G^G[ACGT]CC[CT]
986 GGGCCCT
EcoO109I 425 AGGACCT [GA]G^G[ACGT]CC[CT]
986 GGGCCCT
EcoRII 96 CCTGG ^CC[AT]GG
606 CCTGG
HindIII 285 AAGCTT A^AGCTT
1169 AAGCTT
MflI 684 GGATCT [GA]^GATC[CT]
897 AGATCC
MseI 130 TTAA T^TAA
1313 TTAA
MvaI 96 CCTGG CC^[AT]GG
606 CCTGG
MwoI 278 GCGTGCCAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
932 GCACTTCATGC
NspI 32 ACATGT [GA]CATG^[CT]
1405 ACATGT
ScrFI 96 CCTGG CC^[ACGT]GG
606 CCTGG
Tru9I 130 TTAA T^TAA
1313 TTAA
XbaI 478 TCTAGA T^CTAGA
496 TCTAGA
XhoII 684 GGATCT [GA]^GATC[CT]
897 AGATCC
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BfaI BmyI Bsp1286I BsrDI BsrI
Cac8I Cfr13I DdeI DpnII EarI
HaeIII HinfI HphI Ksp632I MaeI
MaeIII MboI MboII MnlI NdeII
NlaIII PleI RsaI Sau3AI Sau96I
SfcI TaqI TfiI Tsp509I TthHB8I