Restriction map for
pF1KB3755
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AccI AccIII AclI AcsI AflIII
AgeI Alw44I AlwNI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I AspI AviII AvrII BamHI
BbeI BbrPI BbvCI BclI BglII
BlnI BlpI Bpu1102I BsaBI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmI Bsp1407I BspEI BspLU11I BsrGI
Bst1107I BstAPI BstBI BstXI BstZ17I
CelII CpoI DraI DraII DraIII
DrdI EagI EclXI Eco47III Eco52I
EcoO109I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HgaI
HpaI KasI KspI MamI MfeI
MluI MroI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI ScaI
SexAI SfiI SfoI SfuI SgrAI
SpeI SphI SspBI SspI SwaI
Tth111I XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AatII 1324 GACGTC GACGT^C
Acc65I 160 GGTACC G^GTACC
AccII 1750 CGCG CG^CG
AcyI 1324 GACGTC G[GA]^CG[CT]C
AflII 321 CTTAAG C^TTAAG
AlwI 758 GGATC GGATC(4/5)
Asp718I 160 GGTACC G^GTACC
BalI 1388 TGGCCA TGG^CCA
BcgI 701 GCACAGCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 59 GGATAC GTATCC(6/5)
BfaI 1291 CTAG C^TAG
BfrI 321 CTTAAG C^TTAAG
BglI 1381 GCCACTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 1324 GACGTC G[GA]^CG[CT]C
BsmBI 1078 GAGACG CGTCTC(1/5)
BspDI 395 ATCGAT AT^CGAT
BspHI 389 TCATGA T^CATGA
BsrBI 517 CCGCTC CCGCTC(-3/-3)
BsrDI 455 GCAATG GCAATG(2/0)
BsrFI 553 GCCGGT [GA]^CCGG[CT]
BssHII 1749 GCGCGC G^CGCGC
BssSI 510 CACGAG CACGAG(-5/-1)
BstEII 240 GGTGACC G^GT[ACGT]ACC
BstPI 240 GGTGACC G^GT[ACGT]ACC
BstUI 1750 CGCG CG^CG
BstYI 758 GGATCT [GA]^GATC[CT]
Cfr10I 553 GCCGGT [GA]^CCGG[CT]
ClaI 395 ATCGAT AT^CGAT
EarI 1233 GAAGAG CTCTTC(1/4)
EcoO65I 240 GGTGACC G^GT[ACGT]ACC
EcoT14I 236 CCTTGG C^C[AT][AT]GG
Hin1I 1324 GACGTC G[GA]^CG[CT]C
KpnI 160 GGTACC GGTAC^C
Ksp632I 1233 GAAGAG CTCTTC(1/4)
MaeI 1291 CTAG C^TAG
MflI 758 GGATCT [GA]^GATC[CT]
MluNI 1388 TGGCCA TGG^CCA
MscI 1388 TGGCCA TGG^CCA
MvnI 1750 CGCG CG^CG
PaeR7I 128 CTCGAG C^TCGAG
PflMI 950 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1294 GAGTC GAGTC(4/5)
RcaI 389 TCATGA T^CATGA
SapI 1233 GAAGAGC GCTCTTC(1/4)
SbfI 1164 CCTGCAGG CCTGCA^GG
SmaI 90 CCCGGG CCC^GGG
SnaBI 445 TACGTA TAC^GTA
Sse8387I 1164 CCTGCAGG CCTGCA^GG
StuI 1763 AGGCCT AGG^CCT
StyI 236 CCTTGG C^C[AT][AT]GG
Van91I 950 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 128 CTCGAG C^TCGAG
XhoII 758 GGATCT [GA]^GATC[CT]
XmaCI 90 CCCGGG C^CCGGG
XmaI 90 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AhdI 1410 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1727 GACAGTCGGTC
AocI 404 CCTTAGG CC^T[ACGT]AGG
1174 CCTGAGG
AspEI 1410 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1727 GACAGTCGGTC
AspHI 1102 GTGCTC G[AT]GC[AT]^C
1347 GAGCAC
AvaII 981 GGACC G^G[AT]CC
1511 GGACC
BanI 160 GGTACC G^G[CT][GA]CC
1170 GGTGCC
BanII 599 GGGCTC G[GA]GC[CT]^C
682 GAGCCC
BsaAI 445 TACGTA [CT]AC^GT[GA]
522 CACGTA
BsiHKAI 1102 GTGCTC G[AT]GC[AT]^C
1347 GAGCAC
BsmFI 814 GGGAC GGGAC(10/14)
1443 GTCCC
BspMI 1163 ACCTGC ACCTGC(4/8)
1167 GCAGGT
Bsu36I 404 CCTTAGG CC^T[ACGT]AGG
1174 CCTGAGG
DsaI 848 CCACGG C^C[GA][CT]GG
1301 CCGTGG
Eam1105I 1410 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1727 GACAGTCGGTC
Eco81I 404 CCTTAGG CC^T[ACGT]AGG
1174 CCTGAGG
EcoNI 175 CCTCTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1446 CCTGGTCAAGG
Tsp509I 142 AATT ^AATT
267 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AfaI AluI AvaI BbsI
BbvI BcnI BmyI BpmI BpuAI
BsaJI BseRI BsiYI BslI BsmAI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EaeI EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TaqI TfiI Tru9I TseI Tsp45I
TspRI TthHB8I