Restriction map for
pF1KA0994
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# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AgeI AhdI Alw44I AlwNI AocI
ApaLI AscI AseI AsnI Asp718I
AspEI AviII AvrII BbeI BbsI
BclI BfrI BlnI BlpI Bpu1102I
BpuAI BsaBI BsaHI BsiWI BsmBI
BspDI BsrDI BssHII BssSI Bst1107I
BstBI BstZ17I Bsu36I CelII ClaI
DraI DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoRI EcoRV EcoT22I
FbaI FseI FspI Hin1I HindIII
HpaI KasI KpnI MamI MfeI
MluI MseI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PvuII SalI SbfI ScaI
SexAI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tru9I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccIII 875 TCCGGA T^CCGGA
AspI 271 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1043 GGATCC G^GATCC
BbvCI 172 GCTGAGG CCTCAGC(-5/-2)
BciVI 589 GGATAC GTATCC(6/5)
BfaI 1642 CTAG C^TAG
BsaI 795 GGTCTC GGTCTC(1/5)
BsaWI 875 TCCGGA [AT]^CCGG[AT]
BseAI 875 TCCGGA T^CCGGA
Bsp1407I 605 TGTACA T^GTACA
BspEI 875 TCCGGA T^CCGGA
BspHI 893 TCATGA T^CATGA
BspLU11I 539 ACATGT A^CATGT
BsrFI 692 GCCGGC [GA]^CCGG[CT]
BsrGI 605 TGTACA T^GTACA
BstAPI 1983 GCACATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 446 CCATCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 692 GCCGGC [GA]^CCGG[CT]
CpoI 13 CGGACCG CG^G[AT]CCG
DraIII 1984 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
HgaI 367 GACGC GACGC(5/10)
HincII 283 GTCAAC GT[CT]^[GA]AC
HindII 283 GTCAAC GT[CT]^[GA]AC
KspI 734 CCGCGG CCGC^GG
MaeI 1642 CTAG C^TAG
MroI 875 TCCGGA T^CCGGA
MspA1I 734 CCGCGG C[AC]G^C[GT]G
NaeI 692 GCCGGC GCC^GGC
NcoI 1524 CCATGG C^CATGG
NgoMIV 692 GCCGGC G^CCGGC
NspI 539 ACATGT [GA]CATG^[CT]
PflFI 271 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1787 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 78 GACCGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 25 CTGCAG CTGCA^G
PvuI 1035 CGATCG CGAT^CG
RcaI 893 TCATGA T^CATGA
RsrII 13 CGGACCG CG^G[AT]CCG
SacII 734 CCGCGG CCGC^GG
SfiI 427 GGCCCCGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1181 CCCGGG CCC^GGG
SspBI 605 TGTACA T^GTACA
Tth111I 271 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1181 CCCGGG C^CCGGG
XmaI 1181 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 85 GTCTAC GT^[AC][GT]AC
514 GTCTAC
AcsI 1456 AAATTC [GA]^AATT[CT]
1918 AAATTT
Aor51HI 200 AGCGCT AGC^GCT
1634 AGCGCT
ApoI 1456 AAATTC [GA]^AATT[CT]
1918 AAATTT
Asp700I 654 GAACCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1959 GAAGCCCTTC
BalI 182 TGGCCA TGG^CCA
1472 TGGCCA
BglI 428 GCCCCGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1806 GCCATTCGGGC
BglII 350 AGATCT A^GATCT
528 AGATCT
BseRI 175 GAGGAG GAGGAG(10/8)
1825 GAGGAG
BsgI 1366 GTGCAG GTGCAG(16/14)
1612 GTGCAG
BsiEI 573 CGACCG CG[GA][CT]^CG
1035 CGATCG
BsmAI 472 GAGAC GTCTC(1/5)
796 GTCTC
BspMI 1703 ACCTGC ACCTGC(4/8)
1362 GCAGGT
BsrBI 1709 CCGCTC CCGCTC(-3/-3)
10 GAGCGG
BstEII 234 GGTCACC G^GT[ACGT]ACC
276 GGTCACC
BstPI 234 GGTCACC G^GT[ACGT]ACC
276 GGTCACC
EarI 331 CTCTTC CTCTTC(1/4)
1498 CTCTTC
Eco47III 200 AGCGCT AGC^GCT
1634 AGCGCT
EcoO65I 234 GGTCACC G^GT[ACGT]ACC
276 GGTCACC
HaeII 200 AGCGCT [GA]GCGC^[CT]
1634 AGCGCT
Ksp632I 331 CTCTTC CTCTTC(1/4)
1498 CTCTTC
MluNI 182 TGGCCA TGG^CCA
1472 TGGCCA
MscI 182 TGGCCA TGG^CCA
1472 TGGCCA
PflMI 681 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1162 CCAGATTTTGG
SacI 1150 GAGCTC GAGCT^C
1381 GAGCTC
SapI 330 GCTCTTC GCTCTTC(1/4)
1497 GCTCTTC
SmlI 532 CTCAAG C^T[CT][GA]AG
1663 CTCAAG
StuI 759 AGGCCT AGG^CCT
789 AGGCCT
TfiI 1574 GAATC G^A[AT]TC
1590 GATTC
Van91I 681 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1162 CCAGATTTTGG
XmnI 654 GAACCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1959 GAAGCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI ApaI AspHI AvaI AvaII
BanI BanII BbrPI BbvI BcgI
BcnI BmyI BpmI BsaAI BsaJI
BsiHKAI BsiYI BslI BsmFI BsmI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MnlI MslI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PmaCI PmlI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII