Restriction map for
pF1KB8685
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp718I AviII
AvrII BamHI BbrPI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraI Eco47III EcoO65I EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KpnI KspI MaeI MamI
MluI MroI MseI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SapI SbfI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I XbaI XhoI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 1603 GTCGAC GT^[AC][GT]AC
AcsI 1081 GAATTC [GA]^AATT[CT]
AhdI 703 GACCCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 697 CCTGAGG CC^T[ACGT]AGG
ApaI 1445 GGGCCC GGGCC^C
ApoI 1081 GAATTC [GA]^AATT[CT]
Asp700I 1368 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 703 GACCCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1201 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1264 GGCGCC GGCGC^C
BsaI 5 GAGACC GGTCTC(1/5)
BsiEI 1466 CGGCCG CG[GA][CT]^CG
BsrBI 1257 CCGCTC CCGCTC(-3/-3)
BsrFI 940 GCCGGC [GA]^CCGG[CT]
BstXI 905 CCAACATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 697 CCTGAGG CC^T[ACGT]AGG
Cfr10I 940 GCCGGC [GA]^CCGG[CT]
DraIII 738 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1390 GACACGCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1466 CGGCCG C^GGCCG
Eam1105I 703 GACCCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1466 CGGCCG C^GGCCG
Eco52I 1466 CGGCCG C^GGCCG
Eco81I 697 CCTGAGG CC^T[ACGT]AGG
EcoRI 1081 GAATTC G^AATTC
KasI 1264 GGCGCC G^GCGCC
MaeII 1328 ACGT A^CGT
MfeI 633 CAATTG C^AATTG
MunI 633 CAATTG C^AATTG
NaeI 940 GCCGGC GCC^GGC
NarI 1264 GGCGCC GG^CGCC
NgoMIV 940 GCCGGC G^CCGGC
PflFI 1201 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 994 CAGCTG CAG^CTG
SalI 1603 GTCGAC G^TCGAC
ScaI 1853 AGTACT AGT^ACT
SfoI 1264 GGCGCC GGC^GCC
TfiI 1049 GAATC G^A[AT]TC
Tth111I 1201 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1368 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 1316 CGCG CG^CG
1549 CGCG
BalI 983 TGGCCA TGG^CCA
1613 TGGCCA
BbsI 1683 GAAGAC GAAGAC(2/6)
220 GTCTTC
BglI 1256 GCCGCTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1484 GCCCGCTGGGC
BpuAI 1683 GAAGAC GAAGAC(2/6)
220 GTCTTC
BsgI 1659 CTGCAC GTGCAG(16/14)
595 GTGCAG
BstUI 1316 CGCG CG^CG
1549 CGCG
BstYI 680 AGATCC [GA]^GATC[CT]
1040 AGATCC
EarI 1717 GAAGAG CTCTTC(1/4)
2050 GAAGAG
HincII 427 GTCAAC GT[CT]^[GA]AC
1603 GTCGAC
HindII 427 GTCAAC GT[CT]^[GA]AC
1603 GTCGAC
Ksp632I 1717 GAAGAG CTCTTC(1/4)
2050 GAAGAG
MflI 680 AGATCC [GA]^GATC[CT]
1040 AGATCC
MluNI 983 TGGCCA TGG^CCA
1613 TGGCCA
MscI 983 TGGCCA TGG^CCA
1613 TGGCCA
MvnI 1316 CGCG CG^CG
1549 CGCG
NcoI 1309 CCATGG C^CATGG
1610 CCATGG
PpuMI 701 AGGACCC [GA]G^G[AT]CC[CT]
1700 AGGACCT
SacI 337 GAGCTC GAGCT^C
954 GAGCTC
SexAI 659 ACCTGGT A^CC[AT]GGT
1064 ACCTGGT
SmaI 1419 CCCGGG CCC^GGG
1888 CCCGGG
StuI 65 AGGCCT AGG^CCT
608 AGGCCT
Tsp509I 634 AATT ^AATT
1082 AATT
XcmI 800 CCACCTACAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
986 CCATGAAACAGCTGG
XhoII 680 AGATCC [GA]^GATC[CT]
1040 AGATCC
XmaCI 1419 CCCGGG C^CCGGG
1888 CCCGGG
XmaI 1419 CCCGGG C^CCGGG
1888 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BanI
BanII BbvCI BbvI BcnI BmyI
BpmI BsaHI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstAPI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TseI Tsp45I TspRI
TthHB8I Van91I