################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII Acc65I AccII AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AvaI AviII AvrII BamHI BbeI BbrPI BcgI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaHI BsaJI BsiEI BsiWI BsmBI BsoBI BspDI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI BstXI Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MunI MvnI NaeI NarI NciI NcoI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI StuI StyI SwaI Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 69 enzymes) # ################################### AccI 2110 GTATAC GT^[AC][GT]AC AccIII 420 TCCGGA T^CCGGA AflIII 126 ACATGT A^C[GA][CT]GT AlwI 2123 GATCC GGATC(4/5) Asp700I 2094 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 116 GAGCTC G[AT]GC[AT]^C AspI 1248 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 2001 TGGCCA TGG^CCA BanII 116 GAGCTC G[GA]GC[CT]^C BbvCI 1876 GCTGAGG CCTCAGC(-5/-2) BciVI 417 GTATCC GTATCC(6/5) BpmI 1724 CTCCAG CTGGAG(16/14) BsaAI 1405 CACGTA [CT]AC^GT[GA] BsaBI 1819 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1612 GGTCTC GGTCTC(1/5) BsaWI 420 TCCGGA [AT]^CCGG[AT] BseAI 420 TCCGGA T^CCGGA BseRI 1195 CTCCTC GAGGAG(10/8) BsiHKAI 116 GAGCTC G[AT]GC[AT]^C BsmI 578 GAATGC GAATGC(1/-1) Bsp1407I 1557 TGTACA T^GTACA BspEI 420 TCCGGA T^CCGGA BspHI 2061 TCATGA T^CATGA BspLU11I 126 ACATGT A^CATGT BsrGI 1557 TGTACA T^GTACA BssKI 1706 CCTGG ^CC[ACGT]GG Bst1107I 2110 GTATAC GTA^TAC BstNI 1706 CCTGG CC^[AT]GG BstYI 2122 AGATCC [GA]^GATC[CT] BstZ17I 2110 GTATAC GTA^TAC CfoI 33 GCGC GCG^C DraI 705 TTTAAA TTT^AAA DraII 1953 GGGACCC [GA]G^G[ACGT]CC[CT] EarI 82 GAAGAG CTCTTC(1/4) EcoO109I 1953 GGGACCC [GA]G^G[ACGT]CC[CT] EcoRI 1672 GAATTC G^AATTC EcoRII 1706 CCTGG ^CC[AT]GG EcoRV 2154 GATATC GAT^ATC HapII 421 CCGG C^CGG HhaI 33 GCGC GCG^C HinP1I 33 GCGC G^CGC HpaII 421 CCGG C^CGG Ksp632I 82 GAAGAG CTCTTC(1/4) MamI 1819 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 2122 AGATCC [GA]^GATC[CT] MluNI 2001 TGGCCA TGG^CCA MroI 420 TCCGGA T^CCGGA MscI 2001 TGGCCA TGG^CCA MspI 421 CCGG C^CGG MvaI 1706 CCTGG CC^[AT]GG NdeI 2087 CATATG CA^TATG PflFI 1248 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 1749 GAGTC GAGTC(4/5) PpuMI 1953 GGGACCC [GA]G^G[AT]CC[CT] PstI 1213 CTGCAG CTGCA^G RcaI 2061 TCATGA T^CATGA SacI 116 GAGCTC GAGCT^C SbfI 1212 CCTGCAGG CCTGCA^GG ScrFI 1706 CCTGG CC^[ACGT]GG SfaNI 1933 GATGC GCATC(5/9) SpeI 181 ACTAGT A^CTAGT SphI 676 GCATGC GCATG^C Sse8387I 1212 CCTGCAGG CCTGCA^GG SspBI 1557 TGTACA T^GTACA TaqI 1545 TCGA T^CGA Tth111I 1248 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1545 TCGA T^CGA XhoII 2122 AGATCC [GA]^GATC[CT] XmnI 2094 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AciI 4 GCGG CCGC(-3/-1) 175 GCGG AvaII 16 GGACC G^G[AT]CC 1954 GGACC BanI 324 GGCACC G^G[CT][GA]CC 1218 GGCACC BsgI 689 CTGCAC GTGCAG(16/14) 400 GTGCAG BsmAI 411 GTCTC GTCTC(1/5) 1613 GTCTC Cfr13I 16 GGACC G^G[ACGT]CC 1954 GGACC EaeI 1592 TGGCCG [CT]^GGCC[GA] 2001 TGGCCA HaeIII 1593 GGCC GG^CC 2002 GGCC MaeII 271 ACGT A^CGT 1406 ACGT MwoI 1723 GCTCCAGTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1735 GCTGTGTTTGC NheI 433 GCTAGC G^CTAGC 570 GCTAGC PvuII 1000 CAGCTG CAG^CTG 1733 CAGCTG Sau96I 16 GGACC G^G[ACGT]CC 1954 GGACC SmlI 1226 CTCAAG C^T[CT][GA]AG 1831 CTCAAG SspI 1087 AATATT AAT^ATT 1566 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AcsI AfaI AluI ApoI AseI AsnI BbsI BbvI BfaI BmyI BpuAI BsiYI BslI BsmFI Bsp1286I BsrDI BsrI BstF5I Cac8I DdeI DpnII Fnu4HI FokI HinfI HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MslI MspA1I NdeII NlaIII NlaIV NspI PshBI RsaI Sau3AI SfcI TfiI Tru9I TseI Tsp45I Tsp509I TspRI