Restriction map for
pF1KE0013
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII Acc65I AccII AclI AcyI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AvaI AviII AvrII
BamHI BbeI BbrPI BcgI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaHI BsaJI BsiEI
BsiWI BsmBI BsoBI BspDI BspMI
BsrBI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstPI BstUI BstXI
Bsu36I CelII Cfr10I ClaI CpoI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MfeI MluI MunI
MvnI NaeI NarI NciI NcoI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacII SalI SapI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI StuI StyI SwaI
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 69 enzymes) #
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AccI 2110 GTATAC GT^[AC][GT]AC
AccIII 420 TCCGGA T^CCGGA
AflIII 126 ACATGT A^C[GA][CT]GT
AlwI 2123 GATCC GGATC(4/5)
Asp700I 2094 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 116 GAGCTC G[AT]GC[AT]^C
AspI 1248 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2001 TGGCCA TGG^CCA
BanII 116 GAGCTC G[GA]GC[CT]^C
BbvCI 1876 GCTGAGG CCTCAGC(-5/-2)
BciVI 417 GTATCC GTATCC(6/5)
BpmI 1724 CTCCAG CTGGAG(16/14)
BsaAI 1405 CACGTA [CT]AC^GT[GA]
BsaBI 1819 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1612 GGTCTC GGTCTC(1/5)
BsaWI 420 TCCGGA [AT]^CCGG[AT]
BseAI 420 TCCGGA T^CCGGA
BseRI 1195 CTCCTC GAGGAG(10/8)
BsiHKAI 116 GAGCTC G[AT]GC[AT]^C
BsmI 578 GAATGC GAATGC(1/-1)
Bsp1407I 1557 TGTACA T^GTACA
BspEI 420 TCCGGA T^CCGGA
BspHI 2061 TCATGA T^CATGA
BspLU11I 126 ACATGT A^CATGT
BsrGI 1557 TGTACA T^GTACA
BssKI 1706 CCTGG ^CC[ACGT]GG
Bst1107I 2110 GTATAC GTA^TAC
BstNI 1706 CCTGG CC^[AT]GG
BstYI 2122 AGATCC [GA]^GATC[CT]
BstZ17I 2110 GTATAC GTA^TAC
CfoI 33 GCGC GCG^C
DraI 705 TTTAAA TTT^AAA
DraII 1953 GGGACCC [GA]G^G[ACGT]CC[CT]
EarI 82 GAAGAG CTCTTC(1/4)
EcoO109I 1953 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 1672 GAATTC G^AATTC
EcoRII 1706 CCTGG ^CC[AT]GG
EcoRV 2154 GATATC GAT^ATC
HapII 421 CCGG C^CGG
HhaI 33 GCGC GCG^C
HinP1I 33 GCGC G^CGC
HpaII 421 CCGG C^CGG
Ksp632I 82 GAAGAG CTCTTC(1/4)
MamI 1819 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 2122 AGATCC [GA]^GATC[CT]
MluNI 2001 TGGCCA TGG^CCA
MroI 420 TCCGGA T^CCGGA
MscI 2001 TGGCCA TGG^CCA
MspI 421 CCGG C^CGG
MvaI 1706 CCTGG CC^[AT]GG
NdeI 2087 CATATG CA^TATG
PflFI 1248 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1749 GAGTC GAGTC(4/5)
PpuMI 1953 GGGACCC [GA]G^G[AT]CC[CT]
PstI 1213 CTGCAG CTGCA^G
RcaI 2061 TCATGA T^CATGA
SacI 116 GAGCTC GAGCT^C
SbfI 1212 CCTGCAGG CCTGCA^GG
ScrFI 1706 CCTGG CC^[ACGT]GG
SfaNI 1933 GATGC GCATC(5/9)
SpeI 181 ACTAGT A^CTAGT
SphI 676 GCATGC GCATG^C
Sse8387I 1212 CCTGCAGG CCTGCA^GG
SspBI 1557 TGTACA T^GTACA
TaqI 1545 TCGA T^CGA
Tth111I 1248 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1545 TCGA T^CGA
XhoII 2122 AGATCC [GA]^GATC[CT]
XmnI 2094 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
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AciI 4 GCGG CCGC(-3/-1)
175 GCGG
AvaII 16 GGACC G^G[AT]CC
1954 GGACC
BanI 324 GGCACC G^G[CT][GA]CC
1218 GGCACC
BsgI 689 CTGCAC GTGCAG(16/14)
400 GTGCAG
BsmAI 411 GTCTC GTCTC(1/5)
1613 GTCTC
Cfr13I 16 GGACC G^G[ACGT]CC
1954 GGACC
EaeI 1592 TGGCCG [CT]^GGCC[GA]
2001 TGGCCA
HaeIII 1593 GGCC GG^CC
2002 GGCC
MaeII 271 ACGT A^CGT
1406 ACGT
MwoI 1723 GCTCCAGTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1735 GCTGTGTTTGC
NheI 433 GCTAGC G^CTAGC
570 GCTAGC
PvuII 1000 CAGCTG CAG^CTG
1733 CAGCTG
Sau96I 16 GGACC G^G[ACGT]CC
1954 GGACC
SmlI 1226 CTCAAG C^T[CT][GA]AG
1831 CTCAAG
SspI 1087 AATATT AAT^ATT
1566 AATATT
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# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AcsI AfaI AluI ApoI AseI
AsnI BbsI BbvI BfaI BmyI
BpuAI BsiYI BslI BsmFI Bsp1286I
BsrDI BsrI BstF5I Cac8I DdeI
DpnII Fnu4HI FokI HinfI HphI
ItaI MaeI MaeIII MboI MboII
MnlI MseI MslI MspA1I NdeII
NlaIII NlaIV NspI PshBI RsaI
Sau3AI SfcI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI