Restriction map for pF1KE0013
##################################
# NO CUT ENZYMES (  126 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AcyI        
AflII       AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
Asp718I     AspEI       AvaI        AviII       AvrII       
BamHI       BbeI        BbrPI       BcgI        BclI        
BcnI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BsaHI       BsaJI       BsiEI       
BsiWI       BsmBI       BsoBI       BspDI       BspMI       
BsrBI       BsrFI       BssHII      BssSI       BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraIII      DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoT14I     EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MfeI        MluI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NgoMIV      NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       Psp1406I    PvuI        
RsrII       SacII       SalI        SapI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       StuI        StyI        SwaI        
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   69 enzymes) #
###################################

AccI         2110  GTATAC               GT^[AC][GT]AC
AccIII        420  TCCGGA               T^CCGGA
AflIII        126  ACATGT               A^C[GA][CT]GT
AlwI         2123  GATCC                GGATC(4/5)
Asp700I      2094  GAATTATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         116  GAGCTC               G[AT]GC[AT]^C
AspI         1248  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         2001  TGGCCA               TGG^CCA
BanII         116  GAGCTC               G[GA]GC[CT]^C
BbvCI        1876  GCTGAGG              CCTCAGC(-5/-2)
BciVI         417  GTATCC               GTATCC(6/5)
BpmI         1724  CTCCAG               CTGGAG(16/14)
BsaAI        1405  CACGTA               [CT]AC^GT[GA]
BsaBI        1819  GATGATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1612  GGTCTC               GGTCTC(1/5)
BsaWI         420  TCCGGA               [AT]^CCGG[AT]
BseAI         420  TCCGGA               T^CCGGA
BseRI        1195  CTCCTC               GAGGAG(10/8)
BsiHKAI       116  GAGCTC               G[AT]GC[AT]^C
BsmI          578  GAATGC               GAATGC(1/-1)
Bsp1407I     1557  TGTACA               T^GTACA
BspEI         420  TCCGGA               T^CCGGA
BspHI        2061  TCATGA               T^CATGA
BspLU11I      126  ACATGT               A^CATGT
BsrGI        1557  TGTACA               T^GTACA
BssKI        1706  CCTGG                ^CC[ACGT]GG
Bst1107I     2110  GTATAC               GTA^TAC
BstNI        1706  CCTGG                CC^[AT]GG
BstYI        2122  AGATCC               [GA]^GATC[CT]
BstZ17I      2110  GTATAC               GTA^TAC
CfoI           33  GCGC                 GCG^C
DraI          705  TTTAAA               TTT^AAA
DraII        1953  GGGACCC              [GA]G^G[ACGT]CC[CT]
EarI           82  GAAGAG               CTCTTC(1/4)
EcoO109I     1953  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoRI        1672  GAATTC               G^AATTC
EcoRII       1706  CCTGG                ^CC[AT]GG
EcoRV        2154  GATATC               GAT^ATC
HapII         421  CCGG                 C^CGG
HhaI           33  GCGC                 GCG^C
HinP1I         33  GCGC                 G^CGC
HpaII         421  CCGG                 C^CGG
Ksp632I        82  GAAGAG               CTCTTC(1/4)
MamI         1819  GATGATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI         2122  AGATCC               [GA]^GATC[CT]
MluNI        2001  TGGCCA               TGG^CCA
MroI          420  TCCGGA               T^CCGGA
MscI         2001  TGGCCA               TGG^CCA
MspI          421  CCGG                 C^CGG
MvaI         1706  CCTGG                CC^[AT]GG
NdeI         2087  CATATG               CA^TATG
PflFI        1248  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI         1749  GAGTC                GAGTC(4/5)
PpuMI        1953  GGGACCC              [GA]G^G[AT]CC[CT]
PstI         1213  CTGCAG               CTGCA^G
RcaI         2061  TCATGA               T^CATGA
SacI          116  GAGCTC               GAGCT^C
SbfI         1212  CCTGCAGG             CCTGCA^GG
ScrFI        1706  CCTGG                CC^[ACGT]GG
SfaNI        1933  GATGC                GCATC(5/9)
SpeI          181  ACTAGT               A^CTAGT
SphI          676  GCATGC               GCATG^C
Sse8387I     1212  CCTGCAGG             CCTGCA^GG
SspBI        1557  TGTACA               T^GTACA
TaqI         1545  TCGA                 T^CGA
Tth111I      1248  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I      1545  TCGA                 T^CGA
XhoII        2122  AGATCC               [GA]^GATC[CT]
XmnI         2094  GAATTATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AciI            4  GCGG                 CCGC(-3/-1)
              175  GCGG                
AvaII          16  GGACC                G^G[AT]CC
             1954  GGACC               
BanI          324  GGCACC               G^G[CT][GA]CC
             1218  GGCACC              
BsgI          689  CTGCAC               GTGCAG(16/14)
              400  GTGCAG              
BsmAI         411  GTCTC                GTCTC(1/5)
             1613  GTCTC               
Cfr13I         16  GGACC                G^G[ACGT]CC
             1954  GGACC               
EaeI         1592  TGGCCG               [CT]^GGCC[GA]
             2001  TGGCCA              
HaeIII       1593  GGCC                 GG^CC
             2002  GGCC                
MaeII         271  ACGT                 A^CGT
             1406  ACGT                
MwoI         1723  GCTCCAGTTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
             1735  GCTGTGTTTGC         
NheI          433  GCTAGC               G^CTAGC
              570  GCTAGC              
PvuII        1000  CAGCTG               CAG^CTG
             1733  CAGCTG              
Sau96I         16  GGACC                G^G[ACGT]CC
             1954  GGACC               
SmlI         1226  CTCAAG               C^T[CT][GA]AG
             1831  CTCAAG              
SspI         1087  AATATT               AAT^ATT
             1566  AATATT              


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        AseI        
AsnI        BbsI        BbvI        BfaI        BmyI        
BpuAI       BsiYI       BslI        BsmFI       Bsp1286I    
BsrDI       BsrI        BstF5I      Cac8I       DdeI        
DpnII       Fnu4HI      FokI        HinfI       HphI        
ItaI        MaeI        MaeIII      MboI        MboII       
MnlI        MseI        MslI        MspA1I      NdeII       
NlaIII      NlaIV       NspI        PshBI       RsaI        
Sau3AI      SfcI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       
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