Restriction map for
pF1KB7274
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AgeI AhdI AocI
Aor51HI ApaI AscI AseI AsnI
AspEI AspI AvaI AviII AvrII
BamHI BanII BbeI BbrPI BbvCI
BcgI BclI BcnI BfrI BglI
BlnI BsaHI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BspHI BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstXI BstZ17I Bsu36I Cfr10I
ClaI CpoI DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
FauI FbaI FseI FspI HaeII
HapII HgaI Hin1I HpaII KasI
KspI MfeI MluI MroI MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SphI
Sse8387I SspI StuI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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Acc65I 926 GGTACC G^GTACC
Alw44I 1139 GTGCAC G^TGCAC
AlwNI 1694 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1139 GTGCAC G^TGCAC
Asp718I 926 GGTACC G^GTACC
AspHI 1139 GTGCAC G[AT]GC[AT]^C
BalI 841 TGGCCA TGG^CCA
BanI 926 GGTACC G^G[CT][GA]CC
BbsI 1626 GAAGAC GAAGAC(2/6)
BciVI 224 GGATAC GTATCC(6/5)
BglII 1879 AGATCT A^GATCT
BlpI 80 GCTTAGC GC^T[ACGT]AGC
BmyI 1139 GTGCAC G[AGT]GC[ACT]^C
Bpu1102I 80 GCTTAGC GC^T[ACGT]AGC
BpuAI 1626 GAAGAC GAAGAC(2/6)
BsaAI 975 CACGTA [CT]AC^GT[GA]
BsaBI 541 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1833 GGTCTC GGTCTC(1/5)
BseRI 1015 GAGGAG GAGGAG(10/8)
BsiHKAI 1139 GTGCAC G[AT]GC[AT]^C
Bsp1286I 1139 GTGCAC G[AGT]GC[ACT]^C
Bsp1407I 804 TGTACA T^GTACA
BspLU11I 366 ACATGT A^CATGT
BsrDI 1453 GCAATG GCAATG(2/0)
BsrGI 804 TGTACA T^GTACA
BstEII 615 GGTAACC G^GT[ACGT]ACC
BstPI 615 GGTAACC G^GT[ACGT]ACC
CelII 80 GCTTAGC GC^T[ACGT]AGC
CfoI 177 GCGC GCG^C
DraI 1327 TTTAAA TTT^AAA
DraII 899 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 1302 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 841 TGGCCA [CT]^GGCC[GA]
EcoNI 1933 CCTATAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 899 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 615 GGTAACC G^GT[ACGT]ACC
EcoRI 2208 GAATTC G^AATTC
EcoRV 2167 GATATC GAT^ATC
EcoT22I 1456 ATGCAT ATGCA^T
HhaI 177 GCGC GCG^C
HinP1I 177 GCGC G^CGC
HincII 663 GTTAAC GT[CT]^[GA]AC
HindII 663 GTTAAC GT[CT]^[GA]AC
HindIII 1209 AAGCTT A^AGCTT
HpaI 663 GTTAAC GTT^AAC
KpnI 926 GGTACC GGTAC^C
MamI 541 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 841 TGGCCA TGG^CCA
MscI 841 TGGCCA TGG^CCA
MslI 1596 CATGTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NsiI 1456 ATGCAT ATGCA^T
PpuMI 899 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 1682 CAGCTG CAG^CTG
SexAI 209 ACCTGGT A^CC[AT]GGT
SpeI 1615 ACTAGT A^CTAGT
SspBI 804 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AciI 43 CCGC CCGC(-3/-1)
2119 GCGG
AflIII 366 ACATGT A^C[GA][CT]GT
1855 ACACGT
AvaII 900 GGACC G^G[AT]CC
1073 GGTCC
BpmI 1679 CTCCAG CTGGAG(16/14)
1836 CTCCAG
BsmFI 899 GGGAC GGGAC(10/14)
1979 GTCCC
Cac8I 770 GCCAGC GC[ACGT]^[ACGT]GC
1407 GCAAGC
EcoT14I 92 CCAAGG C^C[AT][AT]GG
904 CCAAGG
MaeII 976 ACGT A^CGT
1857 ACGT
MspA1I 1682 CAGCTG C[AC]G^C[GT]G
2117 CAGCGG
PleI 1943 GAGTC GAGTC(4/5)
1977 GAGTC
PstI 172 CTGCAG CTGCA^G
523 CTGCAG
StyI 92 CCAAGG C^C[AT][AT]GG
904 CCAAGG
Tsp45I 380 GTGAC ^GT[GC]AC
1702 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Asp700I BbvI BfaI BsaJI BsiYI
BslI BsmAI BsmI BsrI BssKI
BstF5I BstNI BstYI Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV NspI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XhoII XmnI