Restriction map for
pF1KE0128
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# NO CUT ENZYMES ( 196 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BalI BamHI BanII
BbrPI BbvCI BcgI BciVI BfaI
BfrI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FseI FspI HincII HindII HindIII
HinfI HpaI HphI KpnI Ksp632I
KspI MaeI MaeII MaeIII MamI
MfeI MflI MluI MluNI MroI
MscI MspA1I MunI MvnI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tsp45I
Tsp509I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AciI 19 GCGG CCGC(-3/-1)
AcyI 163 GGCGCC G[GA]^CG[CT]C
BanI 163 GGCGCC G^G[CT][GA]CC
BbeI 163 GGCGCC GGCGC^C
BbsI 81 GAAGAC GAAGAC(2/6)
BclI 110 TGATCA T^GATCA
BcnI 9 CCCGG CC^[GC]GG
BpuAI 81 GAAGAC GAAGAC(2/6)
BsaHI 163 GGCGCC G[GA]^CG[CT]C
BsaJI 168 CCTTGG C^C[ACGT][ACGT]GG
BsiYI 9 CCCGGATAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 9 CCCGGATAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 185 GGGAC GGGAC(10/14)
BstF5I 173 GGATG GGATG(2/0)
BstNI 260 CCTGG CC^[AT]GG
Cac8I 237 GCTGGC GC[ACGT]^[ACGT]GC
Cfr13I 240 GGCCC G^G[ACGT]CC
DdeI 44 CTGAG C^T[ACGT]AG
DpnII 111 GATC ^GATC
EcoRII 260 CCTGG ^CC[AT]GG
EcoT14I 168 CCTTGG C^C[AT][AT]GG
FbaI 110 TGATCA T^GATCA
FokI 173 GGATG GGATG(9/13)
HapII 10 CCGG C^CGG
HgaI 84 GACGC GACGC(5/10)
Hin1I 163 GGCGCC G[GA]^CG[CT]C
HpaII 10 CCGG C^CGG
KasI 163 GGCGCC G^GCGCC
MboI 111 GATC ^GATC
MseI 106 TTAA T^TAA
MslI 275 CACGACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 10 CCGG C^CGG
MvaI 260 CCTGG CC^[AT]GG
NarI 163 GGCGCC GG^CGCC
NciI 9 CCCGG CC^[GC]GG
NdeII 111 GATC ^GATC
NlaIII 229 CATG CATG^
NlaIV 163 GGCGCC GG[ACGT]^[ACGT]CC
PstI 34 CTGCAG CTGCA^G
Sau3AI 111 GATC ^GATC
Sau96I 240 GGCCC G^G[ACGT]CC
SfaNI 70 GCATC GCATC(5/9)
SfcI 34 CTGCAG C^T[GA][CT]AG
SfoI 163 GGCGCC GGC^GCC
StyI 168 CCTTGG C^C[AT][AT]GG
Tru9I 106 TTAA T^TAA
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# TWO CUT ENZYMES ( 7 enzymes) #
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BbvI 36 GCAGC GCAGC(8/12)
202 GCTGC
BssKI 9 CCCGG ^CC[ACGT]GG
260 CCTGG
HaeIII 240 GGCC GG^CC
258 GGCC
MboII 81 GAAGA GAAGA(8/7)
95 TCTTC
MnlI 40 CCTC CCTC(7/6)
243 CCTC
ScrFI 9 CCCGG CC^[ACGT]GG
260 CCTGG
TseI 36 GCAGC G^C[AT]GC
202 GCTGC
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# 3 OR MORE CUT ENZYMES ( 9 enzymes) #
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AluI CfoI Fnu4HI HaeII HhaI
HinP1I ItaI MwoI TspRI