Restriction map for
pF1KA1032
##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AscI AseI AsnI
Asp718I AspI AviII AvrII BbeI
BbrPI BciVI BclI BfaI BfrI
BlnI BseAI BsmBI BspDI BspEI
BspLU11I BsrFI Bst1107I BstZ17I Cfr10I
ClaI CpoI DraI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KasI KpnI KspI MaeI MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NheI NotI NsiI
PacI PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacII SalI ScaI SexAI SfoI
SgrAI SnaBI SpeI StuI SwaI
Tth111I XbaI XcmI
###################################
# ONE CUT ENZYMES ( 25 enzymes) #
###################################
Alw44I 1647 GTGCAC G^TGCAC
AocI 1252 CCTGAGG CC^T[ACGT]AGG
ApaLI 1647 GTGCAC G^TGCAC
BbvCI 762 CCTCAGC CCTCAGC(-5/-2)
BsaBI 2031 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 65 CGTACG C^GTACG
BsmI 3758 GCATTC GAATGC(1/-1)
BsrDI 3989 GCAATG GCAATG(2/0)
BssHII 4879 GCGCGC G^CGCGC
BstEII 1950 GGTGACC G^GT[ACGT]ACC
BstPI 1950 GGTGACC G^GT[ACGT]ACC
Bsu36I 1252 CCTGAGG CC^T[ACGT]AGG
Eco81I 1252 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1950 GGTGACC G^GT[ACGT]ACC
EcoRI 3189 GAATTC G^AATTC
HincII 4711 GTCAAC GT[CT]^[GA]AC
HindII 4711 GTCAAC GT[CT]^[GA]AC
MamI 2031 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NruI 1730 TCGCGA TCG^CGA
PaeR7I 3616 CTCGAG C^TCGAG
PshAI 4825 GACGCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 858 CCTGCAGG CCTGCA^GG
SfiI 1194 GGCCAAGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 858 CCTGCAGG CCTGCA^GG
XhoI 3616 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 1653 GACGCC G[GA]^CG[CT]C
2608 GGCGTC
Aor51HI 2792 AGCGCT AGC^GCT
3247 AGCGCT
BamHI 236 GGATCC G^GATCC
2083 GGATCC
BcgI 98 GCACGACCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2693 GCACCCTGCTCG
BsaAI 63 CACGTA [CT]AC^GT[GA]
67 TACGTG
BsaHI 1653 GACGCC G[GA]^CG[CT]C
2608 GGCGTC
BsaI 202 GGTCTC GGTCTC(1/5)
1450 GGTCTC
BsaWI 1853 ACCGGA [AT]^CCGG[AT]
2852 ACCGGA
Bsp1407I 3053 TGTACA T^GTACA
4536 TGTACA
BspHI 2687 TCATGA T^CATGA
3764 TCATGA
BsrBI 4960 CCGCTC CCGCTC(-3/-3)
883 GAGCGG
BsrGI 3053 TGTACA T^GTACA
4536 TGTACA
BstBI 1666 TTCGAA TT^CGAA
3808 TTCGAA
DrdI 298 GACTCCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2092 GACCCCTATGTC
EagI 4696 CGGCCG C^GGCCG
4965 CGGCCG
EclXI 4696 CGGCCG C^GGCCG
4965 CGGCCG
Eco47III 2792 AGCGCT AGC^GCT
3247 AGCGCT
Eco52I 4696 CGGCCG C^GGCCG
4965 CGGCCG
EcoNI 993 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3966 CCTTAGCCAGG
Hin1I 1653 GACGCC G[GA]^CG[CT]C
2608 GGCGTC
NspV 1666 TTCGAA TT^CGAA
3808 TTCGAA
RcaI 2687 TCATGA T^CATGA
3764 TCATGA
SfuI 1666 TTCGAA TT^CGAA
3808 TTCGAA
SspBI 3053 TGTACA T^GTACA
4536 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AhdI AluI AlwI AlwNI ApaI
ApoI Asp700I AspEI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BglI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
Eam1105I EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
SphI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmaCI XmaI
XmnI