Restriction map for
pF1KE0133
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AgeI AhdI
AlwI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvrII BamHI
BanII BbvCI BcgI BciVI BclI
BcnI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BsaI BsaWI BseAI
BseRI BsiEI BsiWI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DpnII DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
HincII HindII HindIII HpaI KpnI
MamI MboI MfeI MflI MroI
MseI MslI MunI NciI NdeI
NdeII NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmeI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacI SalI
SapI Sau3AI SbfI ScaI SexAI
SfcI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tru9I Tsp509I Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 27 enzymes) #
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Alw44I 28 GTGCAC G^TGCAC
ApaLI 28 GTGCAC G^TGCAC
AviII 536 TGCGCA TGC^GCA
BbsI 774 GAAGAC GAAGAC(2/6)
BfaI 397 CTAG C^TAG
BpmI 703 CTCCAG CTGGAG(16/14)
BpuAI 774 GAAGAC GAAGAC(2/6)
BsmFI 242 GTCCC GGGAC(10/14)
BssSI 175 CTCGTG CACGAG(-5/-1)
BstXI 759 CCATCCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 579 CTTAG C^T[ACGT]AG
DraII 240 GGGTCCC [GA]G^G[ACGT]CC[CT]
EarI 655 CTCTTC CTCTTC(1/4)
EcoO109I 240 GGGTCCC [GA]G^G[ACGT]CC[CT]
FseI 135 GGCCGGCC GGCCGG^CC
FspI 536 TGCGCA TGC^GCA
HphI 747 TCACC GGTGA(8/7)
Ksp632I 655 CTCTTC CTCTTC(1/4)
MaeI 397 CTAG C^TAG
MluI 778 ACGCGT A^CGCGT
NaeI 136 GCCGGC GCC^GGC
NcoI 272 CCATGG C^CATGG
NgoMIV 136 GCCGGC G^CCGGC
PpuMI 240 GGGTCCC [GA]G^G[AT]CC[CT]
SfaNI 474 GCATC GCATC(5/9)
StuI 20 AGGCCT AGG^CCT
XcmI 543 CCACAGGCACTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AflIII 260 ACGTGT A^C[GA][CT]GT
778 ACGCGT
AlwNI 298 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
529 CAGGCGCTG
BalI 155 TGGCCA TGG^CCA
449 TGGCCA
BbeI 126 GGCGCC GGCGC^C
323 GGCGCC
BbrPI 31 CACGTG CAC^GTG
508 CACGTG
BglI 148 GCCTTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
665 GCCTCACAGGC
BsaAI 31 CACGTG [CT]AC^GT[GA]
508 CACGTG
BsgI 214 CTGCAC GTGCAG(16/14)
295 GTGCAG
BsmAI 442 GTCTC GTCTC(1/5)
653 GTCTC
BsmBI 441 CGTCTC CGTCTC(1/5)
652 CGTCTC
BsrFI 43 GCCGGT [GA]^CCGG[CT]
136 GCCGGC
BstF5I 475 CATCC GGATG(2/0)
760 CATCC
Cfr10I 43 GCCGGT [GA]^CCGG[CT]
136 GCCGGC
DraIII 497 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
508 CACGTGGTG
EcoT14I 272 CCATGG C^C[AT][AT]GG
686 CCAAGG
FauI 245 CCCGC CCCGC(4/6)
770 GCGGG
FokI 475 CATCC GGATG(9/13)
760 CATCC
HapII 44 CCGG C^CGG
137 CCGG
HinfI 3 GAATC G^A[ACGT]TC
755 GATTC
HpaII 44 CCGG C^CGG
137 CCGG
KasI 126 GGCGCC G^GCGCC
323 GGCGCC
KspI 332 CCGCGG CCGC^GG
402 CCGCGG
MaeIII 629 GTGAC ^GT[ACGT]AC
695 GTGAC
MboII 774 GAAGA GAAGA(8/7)
656 TCTTC
MluNI 155 TGGCCA TGG^CCA
449 TGGCCA
MscI 155 TGGCCA TGG^CCA
449 TGGCCA
MspI 44 CCGG C^CGG
137 CCGG
NarI 126 GGCGCC GG^CGCC
323 GGCGCC
PmaCI 31 CACGTG CAC^GTG
508 CACGTG
PmlI 31 CACGTG CAC^GTG
508 CACGTG
SacII 332 CCGCGG CCGC^GG
402 CCGCGG
SfoI 126 GGCGCC GGC^GCC
323 GGCGCC
SmlI 433 CTTGAG C^T[CT][GA]AG
619 CTCAAG
StyI 272 CCATGG C^C[AT][AT]GG
686 CCAAGG
TaqI 83 TCGA T^CGA
488 TCGA
TfiI 3 GAATC G^A[AT]TC
755 GATTC
Tsp45I 629 GTGAC ^GT[GC]AC
695 GTGAC
TthHB8I 83 TCGA T^CGA
488 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AccII AciI AcyI AluI AspHI
AvaII BanI BbvI BmyI BsaHI
BsaJI BsiHKAI BsiYI BslI Bsp1286I
BsrI BssKI BstAPI BstNI BstUI
Cac8I CfoI Cfr13I DsaI EaeI
EcoRII Fnu4HI HaeII HaeIII HgaI
HhaI Hin1I HinP1I ItaI MaeII
MnlI MspA1I MvaI MvnI MwoI
NlaIII NlaIV Sau96I ScrFI TseI
TspRI