Restriction map for
pF1KB9116
##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccI AccIII AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI Asp700I Asp718I
AspEI AspHI AviII BanI BbeI
BbrPI BbvCI BcgI BclI BfrI
BglI BsaI BseAI BsiEI BsiHKAI
BsiWI BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BssSI
Bst1107I BstEII BstPI BstXI BstZ17I
Cfr10I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV FbaI FseI FspI
HaeII HindIII KasI KpnI KspI
MfeI MluI MroI MunI NaeI
NarI NgoMIV NotI NruI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
SfiI SfoI SgrAI SmaI SpeI
Sse8387I StuI SwaI XbaI XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 69 enzymes) #
###################################
AclI 1191 AACGTT AA^CGTT
AcyI 2292 GGCGTC G[GA]^CG[CT]C
AlwNI 2197 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1163 CCTTAGG CC^T[ACGT]AGG
AseI 1680 ATTAAT AT^TAAT
AsnI 1680 ATTAAT AT^TAAT
AspI 621 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 157 CCCGAG C^[CT]CG[GA]G
AvrII 235 CCTAGG C^CTAGG
BalI 2043 TGGCCA TGG^CCA
BamHI 127 GGATCC G^GATCC
BanII 1169 GAGCCC G[GA]GC[CT]^C
BbsI 99 GTCTTC GAAGAC(2/6)
BcnI 1700 CCCGG CC^[GC]GG
BlnI 235 CCTAGG C^CTAGG
BlpI 532 GCTCAGC GC^T[ACGT]AGC
BmyI 1169 GAGCCC G[AGT]GC[ACT]^C
Bpu1102I 532 GCTCAGC GC^T[ACGT]AGC
BpuAI 99 GTCTTC GAAGAC(2/6)
BsaAI 1459 TACGTA [CT]AC^GT[GA]
BsaBI 600 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2292 GGCGTC G[GA]^CG[CT]C
BsmBI 735 GAGACG CGTCTC(1/5)
BsoBI 157 CCCGAG C^[CT]CG[GA]G
Bsp1286I 1169 GAGCCC G[AGT]GC[ACT]^C
Bsp1407I 1621 TGTACA T^GTACA
BsrGI 1621 TGTACA T^GTACA
BssHII 281 GCGCGC G^CGCGC
BstAPI 756 GCAAAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1848 TTCGAA TT^CGAA
Bsu36I 1163 CCTTAGG CC^T[ACGT]AGG
CelII 532 GCTCAGC GC^T[ACGT]AGC
DraII 1055 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 1904 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1046 CCATGG C^C[GA][CT]GG
EaeI 2043 TGGCCA [CT]^GGCC[GA]
Eco81I 1163 CCTTAGG CC^T[ACGT]AGG
EcoO109I 1055 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 672 GAATTC G^AATTC
EcoT22I 1529 ATGCAT ATGCA^T
FauI 2112 CCCGC CCCGC(4/6)
Hin1I 2292 GGCGTC G[GA]^CG[CT]C
HincII 1188 GTTAAC GT[CT]^[GA]AC
HindII 1188 GTTAAC GT[CT]^[GA]AC
HpaI 1188 GTTAAC GTT^AAC
MamI 600 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2043 TGGCCA TGG^CCA
MscI 2043 TGGCCA TGG^CCA
NciI 1700 CCCGG CC^[GC]GG
NcoI 1046 CCATGG C^CATGG
NdeI 206 CATATG CA^TATG
NheI 459 GCTAGC G^CTAGC
NsiI 1529 ATGCAT ATGCA^T
NspV 1848 TTCGAA TT^CGAA
PflFI 621 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2196 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1680 ATTAAT AT^TAAT
Psp1406I 1191 AACGTT AA^CGTT
PstI 582 CTGCAG CTGCA^G
ScaI 1736 AGTACT AGT^ACT
SexAI 1782 ACCAGGT A^CC[AT]GGT
SfcI 582 CTGCAG C^T[GA][CT]AG
SfuI 1848 TTCGAA TT^CGAA
SnaBI 1459 TACGTA TAC^GTA
SspBI 1621 TGTACA T^GTACA
SspI 1789 AATATT AAT^ATT
Tsp45I 799 GTGAC ^GT[GC]AC
Tth111I 621 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2196 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccII 282 CGCG CG^CG
362 CGCG
BglII 599 AGATCT A^GATCT
1970 AGATCT
BsaWI 1544 ACCGGA [AT]^CCGG[AT]
1935 ACCGGA
BseRI 421 GAGGAG GAGGAG(10/8)
1409 GAGGAG
BsmI 1725 GAATGC GAATGC(1/-1)
576 GCATTC
BstUI 282 CGCG CG^CG
362 CGCG
DraI 1354 TTTAAA TTT^AAA
1731 TTTAAA
EarI 1125 CTCTTC CTCTTC(1/4)
997 GAAGAG
HgaI 122 GCGTC GACGC(5/10)
2293 GCGTC
HphI 331 TCACC GGTGA(8/7)
1476 TCACC
Ksp632I 1125 CTCTTC CTCTTC(1/4)
997 GAAGAG
MaeII 1192 ACGT A^CGT
1460 ACGT
MvnI 282 CGCG CG^CG
362 CGCG
PvuII 487 CAGCTG CAG^CTG
2200 CAGCTG
SmlI 132 CTTGAG C^T[CT][GA]AG
879 CTTGAG
SphI 866 GCATGC GCATG^C
1527 GCATGC
TaqI 1849 TCGA T^CGA
2154 TCGA
TthHB8I 1849 TCGA T^CGA
2154 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BbvI BciVI BfaI
BpmI BsaJI BsgI BsiYI BslI
BsmAI BsmFI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TfiI Tru9I TseI Tsp509I
TspRI XhoII