##################################
# NO CUT ENZYMES (  206 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbvCI       
BbvI        BcgI        BciVI       BclI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        BpmI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiHKAI     BsiWI       BsmAI       BsmBI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BsrI        BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstNI       
BstPI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      ClaI        CpoI        
DdeI        DraI        DraII       DraIII      DrdI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRII      EcoRV       EcoT14I     
EcoT22I     FbaI        FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HinfI       HpaI        KasI        
KpnI        Ksp632I     MaeI        MaeIII      MamI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MseI        MslI        MunI        MvaI        
NaeI        NarI        NcoI        NgoMIV      NheI        
NlaIII      NotI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuII       
RcaI        RsaI        RsrII       SacI        SalI        
SapI        SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
TspRI       Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   25 enzymes) #
###################################

AlwI          138  GATCC                GGATC(4/5)
AvaII         255  GGACC                G^G[AT]CC
BbsI          232  GAAGAC               GAAGAC(2/6)
BcnI           50  CCCGG                CC^[GC]GG
BpuAI         232  GAAGAC               GAAGAC(2/6)
BsaJI          82  CCGCGG               C^C[ACGT][ACGT]GG
BseRI          88  CTCCTC               GAGGAG(10/8)
BsiEI         152  CGATCG               CG[GA][CT]^CG
BsiYI          77  CCGACCCGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           77  CCGACCCGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI         254  GGGAC                GGGAC(10/14)
BssKI          50  CCCGG                ^CC[ACGT]GG
DsaI           82  CCGCGG               C^C[GA][CT]GG
Fnu4HI         84  GCGGC                GC^[ACGT]GC
HphI          325  GGTGA                GGTGA(8/7)
ItaI           84  GCGGC                GC^[ACGT]GC
KspI           82  CCGCGG               CCGC^GG
MspA1I         82  CCGCGG               C[AC]G^C[GT]G
NciI           50  CCCGG                CC^[GC]GG
NdeI          320  CATATG               CA^TATG
NruI          155  TCGCGA               TCG^CGA
PvuI          152  CGATCG               CGAT^CG
SacII          82  CCGCGG               CCGC^GG
ScrFI          50  CCCGG                CC^[ACGT]GG
SfaNI         171  GCATC                GCATC(5/9)


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

AccII          83  CGCG                 CG^CG
              156  CGCG                
AcsI          329  AAATTT               [GA]^AATT[CT]
              340  AAATTC              
AluI           71  AGCT                 AG^CT
              179  AGCT                
ApoI          329  AAATTT               [GA]^AATT[CT]
              340  AAATTC              
BstUI          83  CGCG                 CG^CG
              156  CGCG                
Cac8I          56  GCCTGC               GC[ACGT]^[ACGT]GC
              176  GCCAGC              
Cfr13I         48  GGCCC                G^G[ACGT]CC
              255  GGACC               
FauI           63  CCCGC                CCCGC(4/6)
               81  CCCGC               
MvnI           83  CGCG                 CG^CG
              156  CGCG                
MwoI          101  GCTTCTTGGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              171  GCATCGCCAGC         
Sau96I         48  GGCCC                G^G[ACGT]CC
              255  GGACC               


#########################################
# 3 OR MORE CUT ENZYMES (   16 enzymes) #
#########################################

AciI        BstF5I      DpnII       FokI        HaeIII      
HapII       HpaII       MaeII       MboI        MboII       
MnlI        MspI        NdeII       NlaIV       Sau3AI      
Tsp509I