##################################
# NO CUT ENZYMES (  214 enzymes) #
##################################

Acc65I      AccI        AccII       AccIII      AciI        
AclI        AcsI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BcnI        BfaI        BfrI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaI        BsaWI       BseAI       
BseRI       BsiEI       BsiHKAI     BsiWI       BsiYI       
BslI        BsmAI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstF5I      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       CfoI        ClaI        
CpoI        DdeI        DpnII       DraI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FokI        
FseI        FspI        HaeII       HaeIII      HgaI        
HhaI        HinP1I      HincII      HindII      HindIII     
HinfI       HpaI        HphI        KasI        KpnI        
KspI        MaeI        MaeIII      MamI        MboI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MseI        MslI        MunI        MvnI        
NarI        NciI        NcoI        NdeI        NdeII       
NheI        NlaIII      NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        RcaI        RsaI        RsrII       
SacI        SacII       SalI        Sau3AI      SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TaqI        TfiI        
Tru9I       Tsp45I      Tsp509I     TspRI       Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AatII         156  GACGTC               GACGT^C
AcyI          156  GACGTC               G[GA]^CG[CT]C
AlwNI         171  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaII           3  GGACC                G^G[AT]CC
BglI          110  GCCCTGTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI          131  GTGCCC               G[AGT]GC[ACT]^C
BsaHI         156  GACGTC               G[GA]^CG[CT]C
BsgI          168  GTGCAG               GTGCAG(16/14)
BsmFI         154  GGGAC                GGGAC(10/14)
Bsp1286I      131  GTGCCC               G[AGT]GC[ACT]^C
BsrFI          46  GCCGGC               [GA]^CCGG[CT]
BssKI         135  CCAGG                ^CC[ACGT]GG
BstNI         135  CCAGG                CC^[AT]GG
Cfr10I         46  GCCGGC               [GA]^CCGG[CT]
Cfr13I          3  GGACC                G^G[ACGT]CC
EarI           90  GAAGAG               CTCTTC(1/4)
EcoRII        135  CCAGG                ^CC[AT]GG
HapII          47  CCGG                 C^CGG
Hin1I         156  GACGTC               G[GA]^CG[CT]C
HpaII          47  CCGG                 C^CGG
Ksp632I        90  GAAGAG               CTCTTC(1/4)
MaeII         157  ACGT                 A^CGT
MboII          90  GAAGA                GAAGA(8/7)
MnlI           80  CCTC                 CCTC(7/6)
MspA1I        171  CAGCTG               C[AC]G^C[GT]G
MspI           47  CCGG                 C^CGG
MvaI          135  CCAGG                CC^[AT]GG
NaeI           46  GCCGGC               GCC^GGC
NgoMIV         46  GCCGGC               G^CCGGC
PvuII         171  CAGCTG               CAG^CTG
SapI           90  GAAGAGC              GCTCTTC(1/4)
Sau96I          3  GGACC                G^G[ACGT]CC
ScrFI         135  CCAGG                CC^[ACGT]GG
SfaNI         143  GCATC                GCATC(5/9)


###################################
# TWO CUT ENZYMES (    4 enzymes) #
###################################

AluI           94  AGCT                 AG^CT
              172  AGCT                
BsaJI         134  CCCAGG               C^C[ACGT][ACGT]GG
              135  CCAGGG              
BstAPI         62  GCAAAGAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
               77  GCACCTCCTGC         
Cac8I          20  GCTCGC               GC[ACGT]^[ACGT]GC
               46  GCCGGC              


#########################################
# 3 OR MORE CUT ENZYMES (    6 enzymes) #
#########################################

BbvI        Fnu4HI      ItaI        MwoI        NlaIV       
TseI