Restriction map for
pF1KE1843
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Alw44I ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanI BbeI BbrPI BcgI
BclI BcnI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco52I EcoRI EcoRV EcoT22I FbaI
FseI FspI HapII HgaI Hin1I
HincII HindII HindIII HpaI HpaII
KasI KpnI KspI MfeI MluI
MluNI MroI MscI MslI MspI
MunI MvnI MwoI NaeI NarI
NciI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI SspI StuI SwaI Tsp509I
Tth111I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AflIII 983 ACATGT A^C[GA][CT]GT
AlwNI 594 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 19 CCTGAGG CC^T[ACGT]AGG
Aor51HI 91 AGCGCT AGC^GCT
AvaII 458 GGACC G^G[AT]CC
BbsI 286 GAAGAC GAAGAC(2/6)
BbvCI 87 CCTCAGC CCTCAGC(-5/-2)
BciVI 334 GGATAC GTATCC(6/5)
BpuAI 286 GAAGAC GAAGAC(2/6)
BsaBI 205 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 738 GTGCAG GTGCAG(16/14)
BspLU11I 983 ACATGT A^CATGT
BspMI 500 ACCTGC ACCTGC(4/8)
Bsu36I 19 CCTGAGG CC^T[ACGT]AGG
DsaI 912 CCATGG C^C[GA][CT]GG
Eco47III 91 AGCGCT AGC^GCT
Eco81I 19 CCTGAGG CC^T[ACGT]AGG
FauI 218 GCGGG CCCGC(4/6)
HaeII 91 AGCGCT [GA]GCGC^[CT]
MaeII 320 ACGT A^CGT
MamI 205 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 216 CAGCGG C[AC]G^C[GT]G
NcoI 912 CCATGG C^CATGG
NspI 983 ACATGT [GA]CATG^[CT]
PpuMI 457 GGGACCT [GA]G^G[AT]CC[CT]
SfcI 782 CTACAG C^T[GA][CT]AG
SmlI 305 CTCAAG C^T[CT][GA]AG
SpeI 577 ACTAGT A^CTAGT
TaqI 864 TCGA T^CGA
TthHB8I 864 TCGA T^CGA
XbaI 840 TCTAGA T^CTAGA
XcmI 593 CCAGAGACTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
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AfaI 153 GTAC GT^AC
484 GTAC
Asp700I 52 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
506 GAAGAGATTC
AspHI 302 GAGCTC G[AT]GC[AT]^C
636 GAGCTC
BanII 302 GAGCTC G[GA]GC[CT]^C
636 GAGCTC
BbvI 447 GCAGC GCAGC(8/12)
557 GCAGC
BfaI 578 CTAG C^TAG
841 CTAG
BmyI 302 GAGCTC G[AGT]GC[ACT]^C
636 GAGCTC
BsaI 113 GAGACC GGTCTC(1/5)
412 GGTCTC
BsiHKAI 302 GAGCTC G[AT]GC[AT]^C
636 GAGCTC
Bsp1286I 302 GAGCTC G[AGT]GC[ACT]^C
636 GAGCTC
BstEII 916 GGTGACC G^GT[ACGT]ACC
949 GGTGACC
BstPI 916 GGTGACC G^GT[ACGT]ACC
949 GGTGACC
BstYI 26 AGATCC [GA]^GATC[CT]
630 GGATCT
Cac8I 3 GCAGGC GC[ACGT]^[ACGT]GC
443 GCAAGC
CfoI 92 GCGC GCG^C
555 GCGC
DraII 457 GGGACCT [GA]G^G[ACGT]CC[CT]
896 AGGGCCT
EcoNI 15 CCTCCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
465 CCTCATTAAGG
EcoO109I 457 GGGACCT [GA]G^G[ACGT]CC[CT]
896 AGGGCCT
EcoO65I 916 GGTGACC G^GT[ACGT]ACC
949 GGTGACC
EcoT14I 174 CCAAGG C^C[AT][AT]GG
912 CCATGG
HhaI 92 GCGC GCG^C
555 GCGC
HinP1I 92 GCGC G^CGC
555 GCGC
MaeI 578 CTAG C^TAG
841 CTAG
MflI 26 AGATCC [GA]^GATC[CT]
630 GGATCT
NdeI 653 CATATG CA^TATG
692 CATATG
PflMI 593 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
721 CCAAGTTGTGG
PleI 222 GACTC GAGTC(4/5)
715 GACTC
RsaI 153 GTAC GT^AC
484 GTAC
SacI 302 GAGCTC GAGCT^C
636 GAGCTC
SapI 438 GAAGAGC GCTCTTC(1/4)
829 GAAGAGC
SfaNI 46 GATGC GCATC(5/9)
247 GATGC
StyI 174 CCAAGG C^C[AT][AT]GG
912 CCATGG
TfiI 511 GATTC G^A[AT]TC
523 GAATC
TseI 447 GCAGC G^C[AT]GC
557 GCAGC
Tsp45I 917 GTGAC ^GT[GC]AC
950 GTGAC
TspRI 736 CAGTG CA[GC]TG[ACGT][ACGT]^
1050 CACTG
Van91I 593 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
721 CCAAGTTGTGG
XhoII 26 AGATCC [GA]^GATC[CT]
630 GGATCT
XmnI 52 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
506 GAAGAGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AluI AlwI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsrI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeIII MboI MboII MnlI MseI
MvaI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI Tru9I