Restriction map for
pF1KE0234
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BbrPI
BbsI BbvCI BcgI BfrI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsiEI BsiWI BsmI BsoBI Bsp1407I
BspDI BspHI BspLU11I BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HincII HindII HindIII
HpaI KpnI KspI MaeIII MamI
MfeI MluI MluNI MscI MseI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI TfiI
Tru9I Tsp45I Tth111I TthHB8I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccII 118 CGCG CG^CG
AccIII 191 TCCGGA T^CCGGA
AflIII 537 ACGTGT A^C[GA][CT]GT
AgeI 286 ACCGGT A^CCGGT
AvaII 194 GGACC G^G[AT]CC
BanI 254 GGCGCC G^G[CT][GA]CC
BbeI 254 GGCGCC GGCGC^C
BciVI 471 GGATAC GTATCC(6/5)
BclI 222 TGATCA T^GATCA
BcnI 93 CCCGG CC^[GC]GG
BfaI 211 CTAG C^TAG
BglII 338 AGATCT A^GATCT
BseAI 191 TCCGGA T^CCGGA
BseRI 106 GAGGAG GAGGAG(10/8)
BsgI 175 GTGCAG GTGCAG(16/14)
BsmBI 709 GAGACG CGTCTC(1/5)
BsmFI 361 GTCCC GGGAC(10/14)
BspEI 191 TCCGGA T^CCGGA
BsrBI 265 GAGCGG CCGCTC(-3/-3)
BsrDI 576 CATTGC GCAATG(2/0)
BsrFI 286 ACCGGT [GA]^CCGG[CT]
BssSI 103 CACGAG CACGAG(-5/-1)
BstAPI 301 GCAGAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 118 CGCG CG^CG
BstXI 581 CCAAGCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 338 AGATCT [GA]^GATC[CT]
Cfr10I 286 ACCGGT [GA]^CCGG[CT]
DsaI 555 CCATGG C^C[GA][CT]GG
EaeI 2 TGGCCG [CT]^GGCC[GA]
EcoT14I 555 CCATGG C^C[AT][AT]GG
FbaI 222 TGATCA T^GATCA
HgaI 79 GACGC GACGC(5/10)
HinfI 462 GAGTC G^A[ACGT]TC
KasI 254 GGCGCC G^GCGCC
MaeI 211 CTAG C^TAG
MaeII 537 ACGT A^CGT
MflI 338 AGATCT [GA]^GATC[CT]
MroI 191 TCCGGA T^CCGGA
MslI 168 CACCTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 66 CAGCTG C[AC]G^C[GT]G
MvnI 118 CGCG CG^CG
NarI 254 GGCGCC GG^CGCC
NciI 93 CCCGG CC^[GC]GG
NcoI 555 CCATGG C^CATGG
NspI 585 GCATGT [GA]CATG^[CT]
PinAI 286 ACCGGT A^CCGGT
PleI 462 GAGTC GAGTC(4/5)
PvuII 66 CAGCTG CAG^CTG
SacI 485 GAGCTC GAGCT^C
SfcI 396 CTACAG C^T[GA][CT]AG
SfoI 254 GGCGCC GGC^GCC
StyI 555 CCATGG C^C[AT][AT]GG
Tsp509I 656 AATT ^AATT
XhoII 338 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcyI 79 GACGCC G[GA]^CG[CT]C
254 GGCGCC
AfaI 75 GTAC GT^AC
541 GTAC
Asp700I 57 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
508 GAAATAGTTC
AspHI 39 GAGCAC G[AT]GC[AT]^C
485 GAGCTC
BglI 92 GCCCGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
566 GCCTCCAGGGC
BsaHI 79 GACGCC G[GA]^CG[CT]C
254 GGCGCC
BsaI 289 GGTCTC GGTCTC(1/5)
595 GGTCTC
BsaWI 191 TCCGGA [AT]^CCGG[AT]
286 ACCGGT
BsiHKAI 39 GAGCAC G[AT]GC[AT]^C
485 GAGCTC
BspMI 626 ACCTGC ACCTGC(4/8)
419 GCAGGT
BstF5I 388 CATCC GGATG(2/0)
702 GGATG
DdeI 88 CTGAG C^T[ACGT]AG
171 CTCAG
FauI 97 GCGGG CCCGC(4/6)
122 GCGGG
FokI 388 CATCC GGATG(9/13)
702 GGATG
Hin1I 79 GACGCC G[GA]^CG[CT]C
254 GGCGCC
RsaI 75 GTAC GT^AC
541 GTAC
SfaNI 139 GATGC GCATC(5/9)
214 GATGC
XcmI 686 CCATGCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
725 CCATAGAAGCTGTGG
XmnI 57 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
508 GAAATAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AluI AlwI AlwNI BanII
BbvI BmyI BpmI BsaJI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstNI Cac8I CfoI Cfr13I DpnII
EarI EcoRII Fnu4HI HaeII HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI Ksp632I MboI MboII MnlI
MspI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI TseI
TspRI