Restriction map for
pF1KE0238
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# NO CUT ENZYMES ( 184 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AvaI AviII AvrII BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaJI BsaWI BseAI
BsiEI BsiWI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstF5I BstPI BstUI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DdeI
DraI DraII DrdI DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FokI
FseI FspI HaeII HincII HindII
HindIII HinfI HpaI HphI KasI
KpnI Ksp632I KspI MaeI MamI
MfeI MluI MroI MseI MspA1I
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI TaqI
TfiI Tru9I TthHB8I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AcyI 367 GGCGTC G[GA]^CG[CT]C
AluI 132 AGCT AG^CT
AspHI 340 GAGCAC G[AT]GC[AT]^C
AspI 382 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 99 GGTCC G^G[AT]CC
BamHI 452 GGATCC G^GATCC
BmyI 340 GAGCAC G[AGT]GC[ACT]^C
BpmI 128 CTGGAG CTGGAG(16/14)
BsaAI 79 TACGTG [CT]AC^GT[GA]
BsaHI 367 GGCGTC G[GA]^CG[CT]C
BsaI 32 GAGACC GGTCTC(1/5)
BseRI 289 GAGGAG GAGGAG(10/8)
BsgI 395 GTGCAG GTGCAG(16/14)
BsiHKAI 340 GAGCAC G[AT]GC[AT]^C
BsiYI 22 CCGAGACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 22 CCGAGACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 369 CGTCTC CGTCTC(1/5)
Bsp1286I 340 GAGCAC G[AGT]GC[ACT]^C
BssKI 384 CCAGG ^CC[ACGT]GG
BstAPI 303 GCAGGAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 384 CCAGG CC^[AT]GG
BstYI 452 GGATCC [GA]^GATC[CT]
CfoI 360 GCGC GCG^C
DpnII 453 GATC ^GATC
DraIII 343 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRII 384 CCAGG ^CC[AT]GG
HapII 112 CCGG C^CGG
HgaI 368 GCGTC GACGC(5/10)
HhaI 360 GCGC GCG^C
Hin1I 367 GGCGTC G[GA]^CG[CT]C
HinP1I 360 GCGC G^CGC
HpaII 112 CCGG C^CGG
MaeII 80 ACGT A^CGT
MboI 453 GATC ^GATC
MboII 180 GAAGA GAAGA(8/7)
MflI 452 GGATCC [GA]^GATC[CT]
MnlI 289 GAGG CCTC(7/6)
MslI 75 CATCTACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 112 CCGG C^CGG
MvaI 384 CCAGG CC^[AT]GG
NdeII 453 GATC ^GATC
PflFI 382 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Sau3AI 453 GATC ^GATC
ScrFI 384 CCAGG CC^[ACGT]GG
SexAI 383 ACCAGGT A^CC[AT]GGT
SfcI 219 CTACAG C^T[GA][CT]AG
StuI 423 AGGCCT AGG^CCT
Tsp509I 123 AATT ^AATT
Tth111I 382 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 354 CCATCTGCGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 452 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 10 enzymes) #
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AlwI 453 GATCC GGATC(4/5)
452 GGATC
BalI 8 TGGCCA TGG^CCA
461 TGGCCA
BbvI 418 GCAGC GCAGC(8/12)
433 GCTGC
BstXI 74 CCATCTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
384 CCAGGTCACTGG
Cfr13I 71 GGGCC G^G[ACGT]CC
99 GGTCC
MluNI 8 TGGCCA TGG^CCA
461 TGGCCA
MscI 8 TGGCCA TGG^CCA
461 TGGCCA
NlaIV 108 GGCTCC GG[ACGT]^[ACGT]CC
452 GGATCC
Sau96I 71 GGGCC G^G[ACGT]CC
99 GGTCC
TseI 418 GCAGC G^C[AT]GC
433 GCTGC
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# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
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AciI BsmAI BsrI Cac8I EaeI
FauI Fnu4HI HaeIII ItaI MaeIII
MwoI Tsp45I TspRI