Restriction map for
pF1KE0243
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# NO CUT ENZYMES ( 146 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspHI AvaI AviII AvrII
BalI BanII BbeI BbrPI BcgI
BclI BfaI BfrI BlnI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmI BsoBI
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I ClaI CpoI DraI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HgaI
Hin1I HindIII HpaI KasI KpnI
KspI MaeI MaeII MamI MfeI
MluI MluNI MroI MscI MseI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI StyI SwaI Tru9I Tsp45I
Tsp509I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AocI 358 CCTGAGG CC^T[ACGT]AGG
Asp700I 114 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 1082 GGATCC G^GATCC
BciVI 439 GGATAC GTATCC(6/5)
BcnI 578 CCGGG CC^[GC]GG
BglI 1153 GCCCCCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 482 AGATCT A^GATCT
BlpI 932 GCTCAGC GC^T[ACGT]AGC
BmyI 304 GTGCCC G[AGT]GC[ACT]^C
Bpu1102I 932 GCTCAGC GC^T[ACGT]AGC
BsgI 333 CTGCAC GTGCAG(16/14)
BsmBI 499 GAGACG CGTCTC(1/5)
BsmFI 58 GTCCC GGGAC(10/14)
Bsp1286I 304 GTGCCC G[AGT]GC[ACT]^C
Bsp1407I 50 TGTACA T^GTACA
BsrDI 867 CATTGC GCAATG(2/0)
BsrGI 50 TGTACA T^GTACA
Bsu36I 358 CCTGAGG CC^T[ACGT]AGG
CelII 932 GCTCAGC GC^T[ACGT]AGC
DraIII 995 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 916 GACATGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 358 CCTGAGG CC^T[ACGT]AGG
EcoNI 108 CCTGCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 826 GTTGAC GT[CT]^[GA]AC
HindII 826 GTTGAC GT[CT]^[GA]AC
MslI 774 CATCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 927 CAGCTG C[AC]G^C[GT]G
NciI 578 CCGGG CC^[GC]GG
PstI 1012 CTGCAG CTGCA^G
PvuII 927 CAGCTG CAG^CTG
SexAI 1139 ACCAGGT A^CC[AT]GGT
SfcI 1012 CTGCAG C^T[GA][CT]AG
SmlI 81 CTTGAG C^T[CT][GA]AG
SspBI 50 TGTACA T^GTACA
XmnI 114 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AccI 313 GTCTAC GT^[AC][GT]AC
643 GTCTAC
AciI 348 CCGC CCGC(-3/-1)
1104 CCGC
AlwI 1083 GATCC GGATC(4/5)
1082 GGATC
AlwNI 196 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
613 CAGGGCCTG
AspI 445 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
955 GACATCGTC
AvaII 57 GGTCC G^G[AT]CC
630 GGACC
BbvCI 648 CCTCAGC CCTCAGC(-5/-2)
805 GCTGAGG
BseRI 288 CTCCTC GAGGAG(10/8)
573 CTCCTC
BstYI 482 AGATCT [GA]^GATC[CT]
1082 GGATCC
DsaI 764 CCGTGG C^C[GA][CT]GG
1157 CCGTGG
EarI 634 CTCTTC CTCTTC(1/4)
699 CTCTTC
HaeII 30 GGCGCT [GA]GCGC^[CT]
652 AGCGCC
Ksp632I 634 CTCTTC CTCTTC(1/4)
699 CTCTTC
MaeIII 736 GTTAC ^GT[ACGT]AC
749 GTTAC
MflI 482 AGATCT [GA]^GATC[CT]
1082 GGATCC
PflFI 445 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
955 GACATCGTC
PflMI 293 CCAACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
612 CCAGGGCCTGG
PleI 697 GACTC GAGTC(4/5)
977 GAGTC
PpuMI 56 AGGTCCC [GA]G^G[AT]CC[CT]
629 AGGACCT
TfiI 364 GATTC G^A[AT]TC
1066 GAATC
Tth111I 445 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
955 GACATCGTC
Van91I 293 CCAACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
612 CCAGGGCCTGG
XhoII 482 AGATCT [GA]^GATC[CT]
1082 GGATCC
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# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AfaI AluI BanI BbsI BbvI
BpmI BpuAI BsaJI BsiYI BslI
BsmAI BsrFI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MboI MboII MnlI MspI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
TaqI TseI TspRI TthHB8I