Restriction map for
pF1KE0254
##################################
# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AvaII AviII AvrII BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmFI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII CfoI Cfr10I Cfr13I
ClaI CpoI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeII MamI MfeI
MluI MroI MslI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI Sau96I SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
StuI StyI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 40 GTAGAC GT^[AC][GT]AC
AccII 852 CGCG CG^CG
AciI 853 GCGG CCGC(-3/-1)
AfaI 449 GTAC GT^AC
Asp700I 387 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 281 TGGCCA TGG^CCA
BbvI 937 GCAGC GCAGC(8/12)
BcnI 907 CCGGG CC^[GC]GG
BfaI 820 CTAG C^TAG
BglII 714 AGATCT A^GATCT
BseRI 406 CTCCTC GAGGAG(10/8)
BsmAI 523 GAGAC GTCTC(1/5)
BsmI 252 GAATGC GAATGC(1/-1)
BspHI 599 TCATGA T^CATGA
BsrI 279 ACTGG ACTGG(1/-1)
BstAPI 520 GCAGAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 852 CGCG CG^CG
Cac8I 480 GCTGGC GC[ACGT]^[ACGT]GC
DdeI 829 CTCAG C^T[ACGT]AG
DraI 580 TTTAAA TTT^AAA
DsaI 619 CCGTGG C^C[GA][CT]GG
EaeI 281 TGGCCA [CT]^GGCC[GA]
Fnu4HI 937 GCAGC GC^[ACGT]GC
HaeIII 282 GGCC GG^CC
HapII 907 CCGG C^CGG
HgaI 811 GACGC GACGC(5/10)
HpaII 907 CCGG C^CGG
ItaI 937 GCAGC GC^[ACGT]GC
MaeI 820 CTAG C^TAG
MaeIII 680 GTCAC ^GT[ACGT]AC
MluNI 281 TGGCCA TGG^CCA
MscI 281 TGGCCA TGG^CCA
MspA1I 938 CAGCTG C[AC]G^C[GT]G
MspI 907 CCGG C^CGG
MvnI 852 CGCG CG^CG
NciI 907 CCGGG CC^[GC]GG
NspI 44 ACATGC [GA]CATG^[CT]
PstI 247 CTGCAG CTGCA^G
PvuII 938 CAGCTG CAG^CTG
RcaI 599 TCATGA T^CATGA
RsaI 449 GTAC GT^AC
SacI 918 GAGCTC GAGCT^C
SmlI 859 CTCAAG C^T[CT][GA]AG
SpeI 819 ACTAGT A^CTAGT
SspI 699 AATATT AAT^ATT
TseI 937 GCAGC G^C[AT]GC
Tsp45I 680 GTCAC ^GT[GC]AC
XmnI 387 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 10 GAATTT [GA]^AATT[CT]
105 AAATTT
AlwI 243 GATCC GGATC(4/5)
652 GATCC
AlwNI 521 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
874 CAGTATCTG
ApoI 10 GAATTT [GA]^AATT[CT]
105 AAATTT
AspHI 856 GTGCTC G[AT]GC[AT]^C
918 GAGCTC
BanII 802 GAGCCC G[GA]GC[CT]^C
918 GAGCTC
BclI 144 TGATCA T^GATCA
942 TGATCA
BpmI 409 CTCCAG CTGGAG(16/14)
880 CTGGAG
BsiHKAI 856 GTGCTC G[AT]GC[AT]^C
918 GAGCTC
BsrDI 289 GCAATG GCAATG(2/0)
953 GCAATG
BstF5I 555 CATCC GGATG(2/0)
72 GGATG
BstNI 411 CCAGG CC^[AT]GG
558 CCTGG
BstXI 20 CCAATACTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
284 CCAAAGCAATGG
EarI 111 GAAGAG CTCTTC(1/4)
156 GAAGAG
EcoRII 411 CCAGG ^CC[AT]GG
558 CCTGG
FbaI 144 TGATCA T^GATCA
942 TGATCA
FokI 555 CATCC GGATG(9/13)
72 GGATG
Ksp632I 111 GAAGAG CTCTTC(1/4)
156 GAAGAG
MvaI 411 CCAGG CC^[AT]GG
558 CCTGG
MwoI 324 GCTGGTGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
520 GCAGAGACTGC
SfcI 59 CTGTAG C^T[GA][CT]AG
247 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AluI BmyI BsaJI BsiYI BslI
Bsp1286I BssKI BstYI DpnII HinfI
HphI MboI MboII MflI MnlI
MseI NdeII NlaIII NlaIV Sau3AI
ScrFI SfaNI TfiI Tru9I Tsp509I
TspRI XhoII