################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbsI BciVI BclI BcnI BfrI BglII BlnI BpmI BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I CfoI Cfr10I ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI MaeII MamI MfeI MflI MluI MluNI MroI MscI MseI MslI MunI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI TaqI Tru9I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AccII 8 CGCG CG^CG AlwI 506 GGATC GGATC(4/5) AlwNI 668 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 873 CCTCAGG CC^T[ACGT]AGG ApaI 587 GGGCCC GGGCC^C AspHI 214 GAGCTC G[AT]GC[AT]^C AspI 28 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 902 GGCACC G^G[CT][GA]CC BbvCI 58 GCTGAGG CCTCAGC(-5/-2) BcgI 95 GCAAAGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 553 GCCTTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 712 GCTGAGC GC^T[ACGT]AGC Bpu1102I 712 GCTGAGC GC^T[ACGT]AGC BsiEI 105 CGGCCG CG[GA][CT]^CG BsiHKAI 214 GAGCTC G[AT]GC[AT]^C BsmBI 565 CGTCTC CGTCTC(1/5) BsrBI 12 GAGCGG CCGCTC(-3/-3) BsrDI 23 GCAATG GCAATG(2/0) BstAPI 1113 GCAGAGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 8 CGCG CG^CG BstXI 576 CCAGGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 873 CCTCAGG CC^T[ACGT]AGG CelII 712 GCTGAGC GC^T[ACGT]AGC DpnII 507 GATC ^GATC DsaI 7 CCGCGG C^C[GA][CT]GG EagI 105 CGGCCG C^GGCCG EclXI 105 CGGCCG C^GGCCG Eco52I 105 CGGCCG C^GGCCG Eco81I 873 CCTCAGG CC^T[ACGT]AGG EcoNI 282 CCTTTCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 185 CCAAGG C^C[AT][AT]GG HapII 108 CCGG C^CGG HgaI 814 GACGC GACGC(5/10) HindIII 523 AAGCTT A^AGCTT HpaII 108 CCGG C^CGG KspI 7 CCGCGG CCGC^GG MaeIII 142 GTGAC ^GT[ACGT]AC MboI 507 GATC ^GATC MspI 108 CCGG C^CGG MvnI 8 CGCG CG^CG NdeI 998 CATATG CA^TATG NdeII 507 GATC ^GATC PflFI 28 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 578 AGGACCC [GA]G^G[AT]CC[CT] PvuII 67 CAGCTG CAG^CTG SacI 214 GAGCTC GAGCT^C SacII 7 CCGCGG CCGC^GG SapI 216 GCTCTTC GCTCTTC(1/4) Sau3AI 507 GATC ^GATC SmlI 89 CTCAAG C^T[CT][GA]AG SphI 1118 GCATGC GCATG^C StyI 185 CCAAGG C^C[AT][AT]GG TfiI 438 GATTC G^A[AT]TC Tsp45I 142 GTGAC ^GT[GC]AC Tsp509I 842 AATT ^AATT Tth111I 28 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 236 CCAACTTGGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AfaI 268 GTAC GT^AC 788 GTAC BanII 214 GAGCTC G[GA]GC[CT]^C 587 GGGCCC BfaI 535 CTAG C^TAG 1060 CTAG BmyI 214 GAGCTC G[AGT]GC[ACT]^C 587 GGGCCC BsaI 292 GAGACC GGTCTC(1/5) 768 GGTCTC BsmFI 429 GTCCC GGGAC(10/14) 870 GTCCC Bsp1286I 214 GAGCTC G[AGT]GC[ACT]^C 587 GGGCCC BsrI 203 ACTGG ACTGG(1/-1) 253 ACTGG EaeI 105 CGGCCG [CT]^GGCC[GA] 233 CGGCCA HphI 141 GGTGA GGTGA(8/7) 1072 GGTGA MaeI 535 CTAG C^TAG 1060 CTAG MspA1I 7 CCGCGG C[AC]G^C[GT]G 67 CAGCTG NspI 708 ACATGC [GA]CATG^[CT] 1118 GCATGC PleI 87 GACTC GAGTC(4/5) 39 GAGTC RsaI 268 GTAC GT^AC 788 GTAC StuI 99 AGGCCT AGG^CCT 551 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AciI AluI AvaII BbvI BsaJI BseRI BsiYI BslI BsmAI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DraII EarI EcoO109I EcoRII Fnu4HI FokI HaeIII HinfI ItaI Ksp632I MboII MnlI MvaI MwoI NlaIII NlaIV PstI Sau96I ScrFI SfaNI SfcI TseI TspRI