Restriction map for
pF1KE0267
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# NO CUT ENZYMES ( 145 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaII AviII
AvrII BamHI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BsaI
BsaWI BseAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I CelII ClaI CpoI DraI
DrdI Eam1105I EarI Eco47III EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HinfI
HpaI KpnI Ksp632I MaeI MamI
MfeI MflI MluI MroI MseI
MunI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsaI RsrII SalI SapI SbfI
ScaI SfaNI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tru9I Tsp509I Tth111I Van91I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AatII 435 GACGTC GACGT^C
AocI 271 CCTGAGG CC^T[ACGT]AGG
ApaI 29 GGGCCC GGGCC^C
AvaI 280 CCCGAG C^[CT]CG[GA]G
BbsI 241 GTCTTC GAAGAC(2/6)
BglI 652 GCCGCCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 241 GTCTTC GAAGAC(2/6)
BsaAI 424 TACGTG [CT]AC^GT[GA]
BsmAI 775 GAGAC GTCTC(1/5)
BsoBI 280 CCCGAG C^[CT]CG[GA]G
BsrDI 730 GCAATG GCAATG(2/0)
BsrFI 361 GCCGGC [GA]^CCGG[CT]
BssSI 331 CTCGTG CACGAG(-5/-1)
BstF5I 54 GGATG GGATG(2/0)
Bsu36I 271 CCTGAGG CC^T[ACGT]AGG
Cfr10I 361 GCCGGC [GA]^CCGG[CT]
DpnII 640 GATC ^GATC
DsaI 685 CCGCGG C^C[GA][CT]GG
Eco81I 271 CCTGAGG CC^T[ACGT]AGG
EcoNI 195 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 236 CCAAGG C^C[AT][AT]GG
FokI 54 GGATG GGATG(9/13)
KspI 685 CCGCGG CCGC^GG
MboI 640 GATC ^GATC
MslI 612 CATCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 685 CCGCGG C[AC]G^C[GT]G
NaeI 361 GCCGGC GCC^GGC
NdeII 640 GATC ^GATC
NgoMIV 361 GCCGGC G^CCGGC
PstI 196 CTGCAG CTGCA^G
SacI 669 GAGCTC GAGCT^C
SacII 685 CCGCGG CCGC^GG
Sau3AI 640 GATC ^GATC
SexAI 164 ACCTGGT A^CC[AT]GGT
SfcI 196 CTGCAG C^T[GA][CT]AG
StuI 922 AGGCCT AGG^CCT
StyI 236 CCAAGG C^C[AT][AT]GG
TaqI 65 TCGA T^CGA
TthHB8I 65 TCGA T^CGA
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# TWO CUT ENZYMES ( 12 enzymes) #
###################################
BcnI 357 CCCGG CC^[GC]GG
702 CCGGG
BseRI 13 CTCCTC GAGGAG(10/8)
127 GAGGAG
BspMI 405 GCAGGT ACCTGC(4/8)
882 GCAGGT
BsrI 717 CCAGT ACTGG(1/-1)
862 CCAGT
DraII 28 GGGGCCC [GA]G^G[ACGT]CC[CT]
29 GGGCCCT
DraIII 163 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
615 CACCATGTG
EcoO109I 28 GGGGCCC [GA]G^G[ACGT]CC[CT]
29 GGGCCCT
HgaI 150 GCGTC GACGC(5/10)
623 GCGTC
MaeIII 678 GTCAC ^GT[ACGT]AC
865 GTCAC
NciI 357 CCCGG CC^[GC]GG
702 CCGGG
Tsp45I 678 GTCAC ^GT[GC]AC
865 GTCAC
TspRI 174 CACTG CA[GC]TG[ACGT][ACGT]^
183 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AcyI AluI AspHI
BalI BanI BanII BbeI BbvI
BmyI BpmI BsaHI BsaJI BsgI
BsiEI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BssKI BstNI BstUI Cac8I
CfoI Cfr13I DdeI EaeI EagI
EclXI Eco52I EcoRII FauI Fnu4HI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HpaII HphI ItaI KasI
MaeII MboII MluNI MnlI MscI
MspI MvaI MvnI MwoI NarI
NlaIII NlaIV Sau96I ScrFI SfoI
TseI XcmI