################################## # NO CUT ENZYMES ( 187 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AfaI AflIII AgeI AhdI Alw44I AlwI AlwNI AocI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AvaII AviII AvrII BalI BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaJI BseAI BseRI BsgI BsiEI BsiWI BsmAI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII Cfr10I Cfr13I ClaI CpoI DpnII DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HpaI KasI KpnI KspI MaeI MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MspA1I MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI Sau3AI Sau96I SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TaqI TfiI Tsp45I TthHB8I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AatII 96 GACGTC GACGT^C AcyI 96 GACGTC G[GA]^CG[CT]C AflII 190 CTTAAG C^TTAAG AluI 188 AGCT AG^CT Aor51HI 42 AGCGCT AGC^GCT AspHI 16 GTGCTC G[AT]GC[AT]^C AspI 234 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 220 GGTGCC G^G[CT][GA]CC BfrI 190 CTTAAG C^TTAAG BmyI 16 GTGCTC G[AGT]GC[ACT]^C BsaHI 96 GACGTC G[GA]^CG[CT]C BsaWI 143 TCCGGT [AT]^CCGG[AT] BsiHKAI 16 GTGCTC G[AT]GC[AT]^C BsiYI 309 CCAGGAAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 309 CCAGGAAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG Bsp1286I 16 GTGCTC G[AGT]GC[ACT]^C BsrBI 165 GAGCGG CCGCTC(-3/-3) BsrDI 33 CATTGC GCAATG(2/0) BsrI 122 CCAGT ACTGG(1/-1) CfoI 43 GCGC GCG^C DdeI 19 CTCAG C^T[ACGT]AG EarI 184 GAAGAG CTCTTC(1/4) Eco47III 42 AGCGCT AGC^GCT EcoRI 304 GAATTC G^AATTC FauI 167 GCGGG CCCGC(4/6) HaeII 42 AGCGCT [GA]GCGC^[CT] HaeIII 103 GGCC GG^CC HapII 144 CCGG C^CGG HhaI 43 GCGC GCG^C Hin1I 96 GACGTC G[GA]^CG[CT]C HinP1I 43 GCGC G^CGC HinfI 238 GAGTC G^A[ACGT]TC HpaII 144 CCGG C^CGG HphI 129 GGTGA GGTGA(8/7) Ksp632I 184 GAAGAG CTCTTC(1/4) MseI 191 TTAA T^TAA MslI 9 CACGGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 144 CCGG C^CGG NlaIV 220 GGTGCC GG[ACGT]^[ACGT]CC PflFI 234 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 309 CCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 238 GAGTC GAGTC(4/5) SapI 184 GAAGAGC GCTCTTC(1/4) SfaNI 5 GCATC GCATC(5/9) SmlI 190 CTTAAG C^T[CT][GA]AG StuI 102 AGGCCT AGG^CCT Tru9I 191 TTAA T^TAA TspRI 21 CAGTG CA[GC]TG[ACGT][ACGT]^ Tth111I 234 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 309 CCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 248 CCAAGTCCTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AcsI 85 AAATTC [GA]^AATT[CT] 304 GAATTC ApoI 85 AAATTC [GA]^AATT[CT] 304 GAATTC BpmI 46 CTCCAG CTGGAG(16/14) 202 CTCCAG BsmI 52 GAATGC GAATGC(1/-1) 321 GCATTC BssKI 48 CCAGG ^CC[ACGT]GG 309 CCAGG BstF5I 135 GGATG GGATG(2/0) 177 GGATG BstNI 48 CCAGG CC^[AT]GG 309 CCAGG EcoRII 48 CCAGG ^CC[AT]GG 309 CCAGG FokI 135 GGATG GGATG(9/13) 177 GGATG MaeII 14 ACGT A^CGT 97 ACGT MvaI 48 CCAGG CC^[AT]GG 309 CCAGG ScrFI 48 CCAGG CC^[ACGT]GG 309 CCAGG ######################################### # 3 OR MORE CUT ENZYMES ( 8 enzymes) # ######################################### AciI BbvI Fnu4HI ItaI MboII MnlI TseI Tsp509I