################################## # NO CUT ENZYMES ( 179 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BsrI BssSI Bst1107I BstBI BstEII BstF5I BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FokI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI MaeI MaeII MaeIII MamI MfeI MluI MluNI MroI MscI MseI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SalI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 20 enzymes) # ################################### Aor51HI 297 AGCGCT AGC^GCT BbsI 18 GAAGAC GAAGAC(2/6) BpuAI 18 GAAGAC GAAGAC(2/6) BsgI 442 CTGCAC GTGCAG(16/14) BsiEI 379 CGACCG CG[GA][CT]^CG BspMI 240 GCAGGT ACCTGC(4/8) BsrBI 190 GAGCGG CCGCTC(-3/-3) BssHII 258 GCGCGC G^CGCGC BstAPI 425 GCAAGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstNI 222 CCAGG CC^[AT]GG Eco47III 297 AGCGCT AGC^GCT EcoNI 342 CCTCCCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRII 222 CCAGG ^CC[AT]GG HgaI 64 GACGC GACGC(5/10) HphI 483 TCACC GGTGA(8/7) MvaI 222 CCAGG CC^[AT]GG NlaIII 10 CATG CATG^ PflMI 237 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SapI 46 GAAGAGC GCTCTTC(1/4) Van91I 237 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AspHI 49 GAGCTC G[AT]GC[AT]^C 412 GAGCTC BanII 49 GAGCTC G[GA]GC[CT]^C 412 GAGCTC BglI 389 GCCGCGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 457 GCCTATGTGGC BmyI 49 GAGCTC G[AGT]GC[ACT]^C 412 GAGCTC BpmI 127 CTGGAG CTGGAG(16/14) 364 CTGGAG BsiHKAI 49 GAGCTC G[AT]GC[AT]^C 412 GAGCTC Bsp1286I 49 GAGCTC G[AGT]GC[ACT]^C 412 GAGCTC BstYI 368 AGATCC [GA]^GATC[CT] 489 GGATCT DdeI 198 CTCAG C^T[ACGT]AG 294 CTTAG DsaI 160 CCGCGG C^C[GA][CT]GG 390 CCGCGG EarI 418 CTCTTC CTCTTC(1/4) 46 GAAGAG HinfI 226 GAGTC G^A[ACGT]TC 438 GAGTC Ksp632I 418 CTCTTC CTCTTC(1/4) 46 GAAGAG KspI 160 CCGCGG CCGC^GG 390 CCGCGG MflI 368 AGATCC [GA]^GATC[CT] 489 GGATCT PleI 226 GAGTC GAGTC(4/5) 438 GAGTC SacI 49 GAGCTC GAGCT^C 412 GAGCTC SacII 160 CCGCGG CCGC^GG 390 CCGCGG TspRI 200 CAGTG CA[GC]TG[ACGT][ACGT]^ 469 CACTG XhoII 368 AGATCC [GA]^GATC[CT] 489 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AccII AciI AluI AlwI BbvI BcnI BsaJI BseRI BsiYI BslI BssKI BstUI Cac8I CfoI Cfr13I DpnII FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HpaII ItaI MboI MboII MnlI MspA1I MspI MvnI MwoI NciI NdeII NlaIV Sau3AI Sau96I ScrFI TseI