################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII Acc65I AclI AcyI AflII AflIII AgeI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspI AvaI AvrII BamHI BanII BbeI BbvCI BbvI BcgI BciVI BclI BfaI BfrI BglI BlnI BlpI BpmI Bpu1102I BsaBI BsaHI BseRI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DdeI DraI DraIII DrdI DsaI EagI EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FbaI Fnu4HI FseI Hin1I HindIII HpaI ItaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MluI MseI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tru9I TseI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 67 enzymes) # ################################### AccII 334 CGCG CG^CG AccIII 537 TCCGGA T^CCGGA AcsI 217 GAATTC [GA]^AATT[CT] AfaI 453 GTAC GT^AC AhdI 343 GACAGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 363 GATCC GGATC(4/5) ApoI 217 GAATTC [GA]^AATT[CT] AspEI 343 GACAGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 301 GAGCAC G[AT]GC[AT]^C AviII 245 TGCGCA TGC^GCA BalI 371 TGGCCA TGG^CCA BanI 595 GGCACC G^G[CT][GA]CC BbrPI 304 CACGTG CAC^GTG BbsI 139 GAAGAC GAAGAC(2/6) BcnI 585 CCGGG CC^[GC]GG BglII 107 AGATCT A^GATCT BpuAI 139 GAAGAC GAAGAC(2/6) BsaAI 304 CACGTG [CT]AC^GT[GA] BsaI 581 GAGACC GGTCTC(1/5) BsaWI 537 TCCGGA [AT]^CCGG[AT] BseAI 537 TCCGGA T^CCGGA BsiHKAI 301 GAGCAC G[AT]GC[AT]^C BsiYI 569 CCAAATGGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 569 CCAAATGGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 581 GAGAC GTCTC(1/5) BsmFI 16 GTCCC GGGAC(10/14) BspEI 537 TCCGGA T^CCGGA BsrDI 609 GCAATG GCAATG(2/0) BssSI 367 CTCGTG CACGAG(-5/-1) BstAPI 237 GCAATACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstNI 410 CCAGG CC^[AT]GG BstUI 334 CGCG CG^CG BstYI 107 AGATCT [GA]^GATC[CT] Cac8I 292 GCCAGC GC[ACGT]^[ACGT]GC DraII 473 AGGACCC [GA]G^G[ACGT]CC[CT] EaeI 371 TGGCCA [CT]^GGCC[GA] Eam1105I 343 GACAGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 473 AGGACCC [GA]G^G[ACGT]CC[CT] EcoRI 217 GAATTC G^AATTC EcoRII 410 CCAGG ^CC[AT]GG EcoT14I 470 CCAAGG C^C[AT][AT]GG FspI 245 TGCGCA TGC^GCA HgaI 335 GCGTC GACGC(5/10) HincII 490 GTCGAC GT[CT]^[GA]AC HindII 490 GTCGAC GT[CT]^[GA]AC MflI 107 AGATCT [GA]^GATC[CT] MluNI 371 TGGCCA TGG^CCA MroI 537 TCCGGA T^CCGGA MscI 371 TGGCCA TGG^CCA MspA1I 203 CAGCTG C[AC]G^C[GT]G MvaI 410 CCAGG CC^[AT]GG MvnI 334 CGCG CG^CG NciI 585 CCGGG CC^[GC]GG NspI 242 ACATGC [GA]CATG^[CT] PmaCI 304 CACGTG CAC^GTG PmlI 304 CACGTG CAC^GTG PpuMI 473 AGGACCC [GA]G^G[AT]CC[CT] PvuII 203 CAGCTG CAG^CTG RsaI 453 GTAC GT^AC SalI 490 GTCGAC G^TCGAC SfaNI 390 GATGC GCATC(5/9) SmlI 396 CTTGAG C^T[CT][GA]AG SspI 445 AATATT AAT^ATT StyI 470 CCAAGG C^C[AT][AT]GG TfiI 534 GATTC G^A[AT]TC XcmI 35 CCACATACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 107 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccI 271 GTCTAC GT^[AC][GT]AC 490 GTCGAC AvaII 309 GGACC G^G[AT]CC 474 GGACC BmyI 80 GTGCCC G[AGT]GC[ACT]^C 301 GAGCAC BsaJI 410 CCAGGG C^C[ACGT][ACGT]GG 470 CCAAGG Bsp1286I 80 GTGCCC G[AGT]GC[ACT]^C 301 GAGCAC BssKI 410 CCAGG ^CC[ACGT]GG 585 CCGGG FauI 231 GCGGG CCCGC(4/6) 576 GCGGG HaeII 10 AGCGCC [GA]GCGC^[CT] 223 AGCGCC HapII 538 CCGG C^CGG 585 CCGG HpaII 538 CCGG C^CGG 585 CCGG HphI 86 TCACC GGTGA(8/7) 407 TCACC MaeII 191 ACGT A^CGT 305 ACGT MaeIII 23 GTGAC ^GT[ACGT]AC 280 GTCAC MspI 538 CCGG C^CGG 585 CCGG NlaIV 474 GGACCC GG[ACGT]^[ACGT]CC 595 GGCACC PleI 142 GACTC GAGTC(4/5) 349 GAGTC ScrFI 410 CCAGG CC^[ACGT]GG 585 CCGGG TaqI 383 TCGA T^CGA 491 TCGA Tsp45I 23 GTGAC ^GT[GC]AC 280 GTCAC Tsp509I 218 AATT ^AATT 522 AATT TthHB8I 383 TCGA T^CGA 491 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AciI AluI BsrI BstF5I CfoI Cfr13I DpnII FokI HaeIII HhaI HinP1I HinfI MboI MboII MnlI MwoI NdeII NlaIII Sau3AI Sau96I TspRI