################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AgeI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspHI AspI AviII AvrII BalI BamHI BanII BbeI BbrPI BcgI BfrI BglI BglII BlnI BmyI BsaBI BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1286I Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MseI MspA1I MunI MvnI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tru9I Tth111I TthHB8I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### Acc65I 392 GGTACC G^GTACC AcsI 331 GAATTC [GA]^AATT[CT] AhdI 253 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 331 GAATTC [GA]^AATT[CT] Asp718I 392 GGTACC G^GTACC AspEI 253 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaII 252 GGACC G^G[AT]CC BanI 392 GGTACC G^G[CT][GA]CC BbvCI 691 GCTGAGG CCTCAGC(-5/-2) BciVI 210 GGATAC GTATCC(6/5) BclI 956 TGATCA T^GATCA BfaI 913 CTAG C^TAG BlpI 189 GCTCAGC GC^T[ACGT]AGC Bpu1102I 189 GCTCAGC GC^T[ACGT]AGC BsaAI 717 TACGTG [CT]AC^GT[GA] BseRI 400 CTCCTC GAGGAG(10/8) BsgI 293 GTGCAG GTGCAG(16/14) BsmFI 235 GTCCC GGGAC(10/14) BsrFI 698 GCCGGC [GA]^CCGG[CT] BstAPI 413 GCAGGGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 101 CCATCTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 189 GCTCAGC GC^T[ACGT]AGC CfoI 702 GCGC GCG^C Cfr10I 698 GCCGGC [GA]^CCGG[CT] DraII 251 AGGACCT [GA]G^G[ACGT]CC[CT] Eam1105I 253 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 258 CCTGTCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 251 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRI 331 GAATTC G^AATTC FauI 161 CCCGC CCCGC(4/6) FbaI 956 TGATCA T^GATCA HhaI 702 GCGC GCG^C HinP1I 702 GCGC G^CGC KpnI 392 GGTACC GGTAC^C MaeI 913 CTAG C^TAG NaeI 698 GCCGGC GCC^GGC NgoMIV 698 GCCGGC G^CCGGC PflMI 729 CCACATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 251 AGGACCT [GA]G^G[AT]CC[CT] PshAI 477 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 893 CTGCAG CTGCA^G ScaI 909 AGTACT AGT^ACT SmaI 73 CCCGGG CCC^GGG SmlI 873 CTTGAG C^T[CT][GA]AG StuI 48 AGGCCT AGG^CCT TfiI 138 GATTC G^A[AT]TC Tsp509I 332 AATT ^AATT TspRI 231 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 729 CCACATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 73 CCCGGG C^CCGGG XmaI 73 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AflIII 572 ACACGT A^C[GA][CT]GT 718 ACGTGT AlwI 509 GATCC GGATC(4/5) 540 GATCC AvaI 73 CCCGGG C^[CT]CG[GA]G 512 CCCGAG BbsI 14 GTCTTC GAAGAC(2/6) 94 GTCTTC BcnI 73 CCCGG CC^[GC]GG 74 CCGGG BpuAI 14 GTCTTC GAAGAC(2/6) 94 GTCTTC BsaI 586 GAGACC GGTCTC(1/5) 346 GGTCTC BsmAI 586 GAGAC GTCTC(1/5) 347 GTCTC BsoBI 73 CCCGGG C^[CT]CG[GA]G 512 CCCGAG BspHI 134 TCATGA T^CATGA 404 TCATGA BstYI 508 AGATCC [GA]^GATC[CT] 539 AGATCC Cfr13I 252 GGACC G^G[ACGT]CC 696 GGGCC EcoT14I 263 CCAAGG C^C[AT][AT]GG 458 CCTTGG HapII 74 CCGG C^CGG 699 CCGG HpaII 74 CCGG C^CGG 699 CCGG MaeIII 484 GTCAC ^GT[ACGT]AC 576 GTCAC MflI 508 AGATCC [GA]^GATC[CT] 539 AGATCC MspI 74 CCGG C^CGG 699 CCGG NciI 73 CCCGG CC^[GC]GG 74 CCGGG PleI 157 GACTC GAGTC(4/5) 477 GACTC RcaI 134 TCATGA T^CATGA 404 TCATGA Sau96I 252 GGACC G^G[ACGT]CC 696 GGGCC StyI 263 CCAAGG C^C[AT][AT]GG 458 CCTTGG Tsp45I 484 GTCAC ^GT[GC]AC 576 GTCAC XcmI 101 CCATCTTTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 275 CCATTTCCTTCCTGG XhoII 508 AGATCC [GA]^GATC[CT] 539 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AciI AfaI AluI BbvI BpmI BsaJI BsiYI BslI BssKI BstF5I BstNI Cac8I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeII MboI MboII MnlI MslI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI ScrFI SfaNI SfcI TseI