Restriction map for
pF1KE0283
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AgeI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I AspHI AspI
AviII AvrII BalI BamHI BanII
BbeI BbrPI BcgI BfrI BglI
BglII BlnI BmyI BsaBI BsaHI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmI Bsp1286I Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrGI BsrI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI EarI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV EcoT22I FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI KasI Ksp632I KspI MamI
MfeI MluI MluNI MroI MscI
MseI MspA1I MunI MvnI NarI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TaqI Tru9I Tth111I
TthHB8I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 392 GGTACC G^GTACC
AcsI 331 GAATTC [GA]^AATT[CT]
AhdI 253 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 331 GAATTC [GA]^AATT[CT]
Asp718I 392 GGTACC G^GTACC
AspEI 253 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 252 GGACC G^G[AT]CC
BanI 392 GGTACC G^G[CT][GA]CC
BbvCI 691 GCTGAGG CCTCAGC(-5/-2)
BciVI 210 GGATAC GTATCC(6/5)
BclI 956 TGATCA T^GATCA
BfaI 913 CTAG C^TAG
BlpI 189 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 189 GCTCAGC GC^T[ACGT]AGC
BsaAI 717 TACGTG [CT]AC^GT[GA]
BseRI 400 CTCCTC GAGGAG(10/8)
BsgI 293 GTGCAG GTGCAG(16/14)
BsmFI 235 GTCCC GGGAC(10/14)
BsrFI 698 GCCGGC [GA]^CCGG[CT]
BstAPI 413 GCAGGGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 101 CCATCTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 189 GCTCAGC GC^T[ACGT]AGC
CfoI 702 GCGC GCG^C
Cfr10I 698 GCCGGC [GA]^CCGG[CT]
DraII 251 AGGACCT [GA]G^G[ACGT]CC[CT]
Eam1105I 253 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 258 CCTGTCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 251 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 331 GAATTC G^AATTC
FauI 161 CCCGC CCCGC(4/6)
FbaI 956 TGATCA T^GATCA
HhaI 702 GCGC GCG^C
HinP1I 702 GCGC G^CGC
KpnI 392 GGTACC GGTAC^C
MaeI 913 CTAG C^TAG
NaeI 698 GCCGGC GCC^GGC
NgoMIV 698 GCCGGC G^CCGGC
PflMI 729 CCACATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 251 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 477 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 893 CTGCAG CTGCA^G
ScaI 909 AGTACT AGT^ACT
SmaI 73 CCCGGG CCC^GGG
SmlI 873 CTTGAG C^T[CT][GA]AG
StuI 48 AGGCCT AGG^CCT
TfiI 138 GATTC G^A[AT]TC
Tsp509I 332 AATT ^AATT
TspRI 231 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 729 CCACATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 73 CCCGGG C^CCGGG
XmaI 73 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflIII 572 ACACGT A^C[GA][CT]GT
718 ACGTGT
AlwI 509 GATCC GGATC(4/5)
540 GATCC
AvaI 73 CCCGGG C^[CT]CG[GA]G
512 CCCGAG
BbsI 14 GTCTTC GAAGAC(2/6)
94 GTCTTC
BcnI 73 CCCGG CC^[GC]GG
74 CCGGG
BpuAI 14 GTCTTC GAAGAC(2/6)
94 GTCTTC
BsaI 586 GAGACC GGTCTC(1/5)
346 GGTCTC
BsmAI 586 GAGAC GTCTC(1/5)
347 GTCTC
BsoBI 73 CCCGGG C^[CT]CG[GA]G
512 CCCGAG
BspHI 134 TCATGA T^CATGA
404 TCATGA
BstYI 508 AGATCC [GA]^GATC[CT]
539 AGATCC
Cfr13I 252 GGACC G^G[ACGT]CC
696 GGGCC
EcoT14I 263 CCAAGG C^C[AT][AT]GG
458 CCTTGG
HapII 74 CCGG C^CGG
699 CCGG
HpaII 74 CCGG C^CGG
699 CCGG
MaeIII 484 GTCAC ^GT[ACGT]AC
576 GTCAC
MflI 508 AGATCC [GA]^GATC[CT]
539 AGATCC
MspI 74 CCGG C^CGG
699 CCGG
NciI 73 CCCGG CC^[GC]GG
74 CCGGG
PleI 157 GACTC GAGTC(4/5)
477 GACTC
RcaI 134 TCATGA T^CATGA
404 TCATGA
Sau96I 252 GGACC G^G[ACGT]CC
696 GGGCC
StyI 263 CCAAGG C^C[AT][AT]GG
458 CCTTGG
Tsp45I 484 GTCAC ^GT[GC]AC
576 GTCAC
XcmI 101 CCATCTTTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
275 CCATTTCCTTCCTGG
XhoII 508 AGATCC [GA]^GATC[CT]
539 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AfaI AluI BbvI BpmI
BsaJI BsiYI BslI BssKI BstF5I
BstNI Cac8I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeII MboI MboII MnlI
MslI MvaI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI ScrFI SfaNI
SfcI TseI