Restriction map for
pF1KE0287
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# NO CUT ENZYMES ( 143 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BalI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmI BsoBI BspDI
BspEI BspMI BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco52I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FseI FspI
HaeIII HgaI Hin1I HindIII HpaI
KasI KpnI KspI MamI MluI
MluNI MroI MscI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I StuI SwaI Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AciI 1218 CCGC CCGC(-3/-1)
AcsI 899 AAATTT [GA]^AATT[CT]
AflIII 518 ACATGT A^C[GA][CT]GT
AocI 531 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1297 AGCGCT AGC^GCT
ApoI 899 AAATTT [GA]^AATT[CT]
Asp700I 897 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 166 GAGCTC G[AT]GC[AT]^C
AvaII 1016 GGTCC G^G[AT]CC
BamHI 948 GGATCC G^GATCC
BanII 166 GAGCTC G[GA]GC[CT]^C
BcnI 444 CCGGG CC^[GC]GG
BmyI 166 GAGCTC G[AGT]GC[ACT]^C
BpmI 18 CTCCAG CTGGAG(16/14)
BseRI 1176 CTCCTC GAGGAG(10/8)
BsiHKAI 166 GAGCTC G[AT]GC[AT]^C
BsmFI 1017 GTCCC GGGAC(10/14)
Bsp1286I 166 GAGCTC G[AGT]GC[ACT]^C
Bsp1407I 117 TGTACA T^GTACA
BspHI 26 TCATGA T^CATGA
BspLU11I 518 ACATGT A^CATGT
BsrDI 622 GCAATG GCAATG(2/0)
BsrGI 117 TGTACA T^GTACA
BsrI 221 ACTGG ACTGG(1/-1)
Bsu36I 531 CCTCAGG CC^T[ACGT]AGG
Cac8I 773 GCAGGC GC[ACGT]^[ACGT]GC
Cfr13I 1016 GGTCC G^G[ACGT]CC
DraI 88 TTTAAA TTT^AAA
DsaI 587 CCATGG C^C[GA][CT]GG
Eco47III 1297 AGCGCT AGC^GCT
Eco81I 531 CCTCAGG CC^T[ACGT]AGG
HaeII 1297 AGCGCT [GA]GCGC^[CT]
HapII 444 CCGG C^CGG
HincII 499 GTCAAC GT[CT]^[GA]AC
HindII 499 GTCAAC GT[CT]^[GA]AC
HpaII 444 CCGG C^CGG
MfeI 82 CAATTG C^AATTG
MspA1I 794 CAGCTG C[AC]G^C[GT]G
MspI 444 CCGG C^CGG
MunI 82 CAATTG C^AATTG
MwoI 573 GCGACTGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 444 CCGGG CC^[GC]GG
NcoI 587 CCATGG C^CATGG
PflMI 406 CCAAAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1249 CTGCAG CTGCA^G
PvuII 794 CAGCTG CAG^CTG
RcaI 26 TCATGA T^CATGA
SacI 166 GAGCTC GAGCT^C
SapI 475 GCTCTTC GCTCTTC(1/4)
Sau96I 1016 GGTCC G^G[ACGT]CC
SfcI 1249 CTGCAG C^T[GA][CT]AG
SspBI 117 TGTACA T^GTACA
TfiI 992 GAATC G^A[AT]TC
Van91I 406 CCAAAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 670 CCATCAGGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 897 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
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AfaI 118 GTAC GT^AC
1167 GTAC
BclI 596 TGATCA T^GATCA
1079 TGATCA
BsmAI 341 GTCTC GTCTC(1/5)
1174 GTCTC
CfoI 78 GCGC GCG^C
1298 GCGC
EarI 476 CTCTTC CTCTTC(1/4)
1315 CTCTTC
FbaI 596 TGATCA T^GATCA
1079 TGATCA
HhaI 78 GCGC GCG^C
1298 GCGC
HinP1I 78 GCGC G^CGC
1298 GCGC
HphI 123 GGTGA GGTGA(8/7)
1266 TCACC
Ksp632I 476 CTCTTC CTCTTC(1/4)
1315 CTCTTC
MaeII 105 ACGT A^CGT
139 ACGT
MslI 513 CACTTACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
588 CATGGATATG
NspI 518 ACATGT [GA]CATG^[CT]
1163 GCATGT
PleI 16 GACTC GAGTC(4/5)
465 GACTC
RsaI 118 GTAC GT^AC
1167 GTAC
SspI 851 AATATT AAT^ATT
919 AATATT
TaqI 14 TCGA T^CGA
933 TCGA
TthHB8I 14 TCGA T^CGA
933 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AluI AlwI BanI BbvI BfaI
BsaJI BsiYI BslI BssKI BstF5I
BstNI BstYI DdeI DpnII EcoRII
EcoT14I Fnu4HI FokI HinfI ItaI
MaeI MaeIII MboI MboII MflI
MnlI MseI MvaI NdeII NlaIII
NlaIV Sau3AI ScrFI SfaNI StyI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII