################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII Acc65I AccII AclI AcsI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BfrI BglI BglII BlnI BsaAI BsiEI BsiWI BsmI BsoBI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HhaI HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TaqI Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccI 488 GTCTAC GT^[AC][GT]AC AccIII 570 TCCGGA T^CCGGA AcyI 831 GACGCC G[GA]^CG[CT]C AflIII 838 ACGTGT A^C[GA][CT]GT AlwNI 1104 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG AseI 733 ATTAAT AT^TAAT AsnI 733 ATTAAT AT^TAAT AvaII 503 GGACC G^G[AT]CC BalI 1184 TGGCCA TGG^CCA BanI 817 GGCACC G^G[CT][GA]CC BbsI 972 GAAGAC GAAGAC(2/6) BfaI 811 CTAG C^TAG BlpI 234 GCTAAGC GC^T[ACGT]AGC Bpu1102I 234 GCTAAGC GC^T[ACGT]AGC BpuAI 972 GAAGAC GAAGAC(2/6) BsaBI 321 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 831 GACGCC G[GA]^CG[CT]C BsaI 535 GGTCTC GGTCTC(1/5) BsaWI 570 TCCGGA [AT]^CCGG[AT] BseAI 570 TCCGGA T^CCGGA BseRI 1118 CTCCTC GAGGAG(10/8) BsmBI 183 CGTCTC CGTCTC(1/5) BspEI 570 TCCGGA T^CCGGA BstAPI 890 GCAACTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 714 CCAAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 234 GCTAAGC GC^T[ACGT]AGC DraII 129 AGGCCCC [GA]G^G[ACGT]CC[CT] DraIII 331 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 248 CCATGG C^C[GA][CT]GG EaeI 1184 TGGCCA [CT]^GGCC[GA] EarI 657 GAAGAG CTCTTC(1/4) EcoNI 281 CCTCTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 129 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoT14I 248 CCATGG C^C[AT][AT]GG Hin1I 831 GACGCC G[GA]^CG[CT]C Ksp632I 657 GAAGAG CTCTTC(1/4) MaeI 811 CTAG C^TAG MamI 321 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1184 TGGCCA TGG^CCA MroI 570 TCCGGA T^CCGGA MscI 1184 TGGCCA TGG^CCA MslI 225 CATGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 248 CCATGG C^CATGG PleI 146 GAGTC GAGTC(4/5) PshBI 733 ATTAAT AT^TAAT SacI 529 GAGCTC GAGCT^C SapI 657 GAAGAGC GCTCTTC(1/4) SspI 736 AATATT AAT^ATT StyI 248 CCATGG C^C[AT][AT]GG Tsp45I 221 GTGAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AfaI 291 GTAC GT^AC 842 GTAC AspHI 18 GAGCAC G[AT]GC[AT]^C 529 GAGCTC BclI 323 TGATCA T^GATCA 1134 TGATCA BcnI 506 CCCGG CC^[GC]GG 513 CCCGG BsgI 177 CTGCAC GTGCAG(16/14) 593 GTGCAG BsiHKAI 18 GAGCAC G[AT]GC[AT]^C 529 GAGCTC BsmFI 631 GGGAC GGGAC(10/14) 710 GTCCC BsrDI 779 CATTGC GCAATG(2/0) 626 GCAATG BstYI 39 GGATCT [GA]^GATC[CT] 394 GGATCT FbaI 323 TGATCA T^GATCA 1134 TGATCA HgaI 831 GACGC GACGC(5/10) 239 GCGTC MaeII 838 ACGT A^CGT 1140 ACGT MaeIII 221 GTGAC ^GT[ACGT]AC 481 GTTAC MflI 39 GGATCT [GA]^GATC[CT] 394 GGATCT MspA1I 59 CAGCGG C[AC]G^C[GT]G 1126 CAGCGG NciI 506 CCCGG CC^[GC]GG 513 CCCGG PflMI 330 CCACAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 414 CCAAGTTATGG RsaI 291 GTAC GT^AC 842 GTAC SfaNI 365 GATGC GCATC(5/9) 602 GCATC SfcI 918 CTGTAG C^T[GA][CT]AG 1214 CTGTAG Tsp509I 947 AATT ^AATT 1024 AATT Van91I 330 CCACAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 414 CCAAGTTATGG XhoII 39 GGATCT [GA]^GATC[CT] 394 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AciI AluI AlwI BanII BbvI BmyI BpmI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EcoRII FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MboI MboII MnlI MseI MspI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TfiI Tru9I TseI TspRI