################################## # NO CUT ENZYMES ( 105 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AcyI AflII AflIII AgeI AhdI AocI ApaI ApoI AscI Asp718I AspEI AvrII BbeI BbrPI BbsI BciVI BfrI BglI BglII BlnI BpuAI BsaAI BsaBI BsaHI BsaI BsgI BsiWI BsmI Bsp1407I BspDI BspLU11I BsrBI BsrFI BsrGI BssHII Bst1107I BstEII BstPI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII Eam1105I EarI Eco81I EcoNI EcoO65I EcoRI EcoRV FseI HgaI Hin1I HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MunI NaeI NarI NdeI NgoMIV NotI NruI PacI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI RsrII SacII SalI SapI SbfI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI Van91I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 63 enzymes) # ################################### AccII 912 CGCG CG^CG Alw44I 1248 GTGCAC G^TGCAC AlwNI 1472 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 992 AGCGCT AGC^GCT ApaLI 1248 GTGCAC G^TGCAC AseI 738 ATTAAT AT^TAAT AsnI 738 ATTAAT AT^TAAT Asp700I 95 GAATCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 1423 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1103 CTCGAG C^[CT]CG[GA]G AviII 1639 TGCGCA TGC^GCA BamHI 1656 GGATCC G^GATCC BanII 126 GAGCTC G[GA]GC[CT]^C BbvCI 10 GCTGAGG CCTCAGC(-5/-2) BcgI 329 GCAAGTACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1113 TGATCA T^GATCA BfaI 1079 CTAG C^TAG BlpI 898 GCTAAGC GC^T[ACGT]AGC Bpu1102I 898 GCTAAGC GC^T[ACGT]AGC BseRI 34 GAGGAG GAGGAG(10/8) BsoBI 1103 CTCGAG C^[CT]CG[GA]G BspMI 63 GCAGGT ACCTGC(4/8) BssSI 123 CACGAG CACGAG(-5/-1) BstAPI 1303 GCAGCCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1488 TTCGAA TT^CGAA BstUI 912 CGCG CG^CG BstXI 626 CCACTGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 898 GCTAAGC GC^T[ACGT]AGC DraII 1400 AGGTCCT [GA]G^G[ACGT]CC[CT] DrdI 232 GACCGATTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 564 CGGCCG C^GGCCG EclXI 564 CGGCCG C^GGCCG Eco47III 992 AGCGCT AGC^GCT Eco52I 564 CGGCCG C^GGCCG EcoO109I 1400 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoT22I 1773 ATGCAT ATGCA^T FbaI 1113 TGATCA T^GATCA FspI 1639 TGCGCA TGC^GCA HincII 808 GTCAAC GT[CT]^[GA]AC HindII 808 GTCAAC GT[CT]^[GA]AC MaeI 1079 CTAG C^TAG MaeII 572 ACGT A^CGT MspA1I 1472 CAGCTG C[AC]G^C[GT]G MvnI 912 CGCG CG^CG NcoI 1652 CCATGG C^CATGG NheI 1078 GCTAGC G^CTAGC NsiI 1773 ATGCAT ATGCA^T NspV 1488 TTCGAA TT^CGAA PaeR7I 1103 CTCGAG C^TCGAG PflFI 1423 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 1400 AGGTCCT [GA]G^G[AT]CC[CT] PshBI 738 ATTAAT AT^TAAT PvuII 1472 CAGCTG CAG^CTG SacI 126 GAGCTC GAGCT^C ScaI 332 AGTACT AGT^ACT SexAI 946 ACCAGGT A^CC[AT]GGT SfcI 429 CTACAG C^T[GA][CT]AG SfuI 1488 TTCGAA TT^CGAA SmlI 1103 CTCGAG C^T[CT][GA]AG StuI 1131 AGGCCT AGG^CCT Tth111I 1423 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 1103 CTCGAG C^TCGAG XmnI 95 GAATCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccIII 222 TCCGGA T^CCGGA 1121 TCCGGA BalI 80 TGGCCA TGG^CCA 638 TGGCCA BanI 55 GGCACC G^G[CT][GA]CC 661 GGCACC BpmI 1560 CTCCAG CTGGAG(16/14) 1213 CTGGAG BsaWI 222 TCCGGA [AT]^CCGG[AT] 1121 TCCGGA BseAI 222 TCCGGA T^CCGGA 1121 TCCGGA BsiEI 231 CGACCG CG[GA][CT]^CG 564 CGGCCG BsmFI 474 GGGAC GGGAC(10/14) 569 GGGAC BspEI 222 TCCGGA T^CCGGA 1121 TCCGGA BspHI 191 TCATGA T^CATGA 368 TCATGA BstYI 210 AGATCC [GA]^GATC[CT] 1656 GGATCC FauI 493 CCCGC CCCGC(4/6) 1611 CCCGC HaeII 524 GGCGCT [GA]GCGC^[CT] 992 AGCGCT MflI 210 AGATCC [GA]^GATC[CT] 1656 GGATCC MluNI 80 TGGCCA TGG^CCA 638 TGGCCA MroI 222 TCCGGA T^CCGGA 1121 TCCGGA MscI 80 TGGCCA TGG^CCA 638 TGGCCA MseI 739 TTAA T^TAA 1532 TTAA RcaI 191 TCATGA T^CATGA 368 TCATGA Tru9I 739 TTAA T^TAA 1532 TTAA XcmI 626 CCACTGCTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1529 CCATTAAACCTCTGG XhoII 210 AGATCC [GA]^GATC[CT] 1656 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI AvaII BbvI BcnI BmyI BsaJI BsiHKAI BsiYI BslI BsmAI BsmBI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SphI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I