Restriction map for
pF1KE1864
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspI AvaI AviII
AvrII BamHI BbrPI BcgI BciVI
BclI BfrI BglI BglII BlnI
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Cfr10I ClaI CpoI DraI
DrdI EagI EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MamI MfeI MluI
MroI MseI MunI MvnI NaeI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI ScaI SexAI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SspBI SspI SwaI TaqI Tru9I
Tth111I TthHB8I XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcsI 498 GAATTT [GA]^AATT[CT]
AfaI 145 GTAC GT^AC
AhdI 711 GACCTGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 9 GGGCCC GGGCC^C
ApoI 498 GAATTT [GA]^AATT[CT]
AspEI 711 GACCTGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 1150 GGCGCC G^G[CT][GA]CC
BbeI 1150 GGCGCC GGCGC^C
BbvCI 139 GCTGAGG CCTCAGC(-5/-2)
BfaI 583 CTAG C^TAG
BlpI 67 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 67 GCTGAGC GC^T[ACGT]AGC
BsaI 253 GAGACC GGTCTC(1/5)
BsgI 121 GTGCAG GTGCAG(16/14)
BsmI 330 GCATTC GAATGC(1/-1)
BssSI 1033 CTCGTG CACGAG(-5/-1)
CelII 67 GCTGAGC GC^T[ACGT]AGC
DraIII 877 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 620 CCATGG C^C[GA][CT]GG
Eam1105I 711 GACCTGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 578 GATATC GAT^ATC
EcoT22I 547 ATGCAT ATGCA^T
HaeII 1150 GGCGCC [GA]GCGC^[CT]
HgaI 717 GCGTC GACGC(5/10)
KasI 1150 GGCGCC G^GCGCC
MaeI 583 CTAG C^TAG
NarI 1150 GGCGCC GG^CGCC
NcoI 620 CCATGG C^CATGG
NsiI 547 ATGCAT ATGCA^T
PflMI 426 CCAAAACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 912 GAGTC GAGTC(4/5)
PvuII 1013 CAGCTG CAG^CTG
RsaI 145 GTAC GT^AC
SapI 414 GCTCTTC GCTCTTC(1/4)
SfoI 1150 GGCGCC GGC^GCC
SphI 549 GCATGC GCATG^C
StuI 644 AGGCCT AGG^CCT
Tsp509I 499 AATT ^AATT
Van91I 426 CCAAAACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 582 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AciI 636 CCGC CCGC(-3/-1)
860 GCGG
AcyI 716 GGCGTC G[GA]^CG[CT]C
1150 GGCGCC
AlwNI 1045 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1108 CAGCCTCTG
AocI 285 CCTCAGG CC^T[ACGT]AGG
600 CCTGAGG
BalI 482 TGGCCA TGG^CCA
803 TGGCCA
BpmI 337 CTCCAG CTGGAG(16/14)
465 CTCCAG
BsaHI 716 GGCGTC G[GA]^CG[CT]C
1150 GGCGCC
BseRI 603 GAGGAG GAGGAG(10/8)
826 GAGGAG
BsmAI 253 GAGAC GTCTC(1/5)
436 GAGAC
BspMI 841 ACCTGC ACCTGC(4/8)
1044 GCAGGT
BstYI 115 GGATCT [GA]^GATC[CT]
359 AGATCC
Bsu36I 285 CCTCAGG CC^T[ACGT]AGG
600 CCTGAGG
CfoI 171 GCGC GCG^C
1151 GCGC
DraII 281 AGGACCT [GA]G^G[ACGT]CC[CT]
1046 AGGTCCT
EaeI 482 TGGCCA [CT]^GGCC[GA]
803 TGGCCA
EarI 415 CTCTTC CTCTTC(1/4)
608 GAAGAG
Eco81I 285 CCTCAGG CC^T[ACGT]AGG
600 CCTGAGG
EcoO109I 281 AGGACCT [GA]G^G[ACGT]CC[CT]
1046 AGGTCCT
FauI 635 CCCGC CCCGC(4/6)
860 GCGGG
HhaI 171 GCGC GCG^C
1151 GCGC
Hin1I 716 GGCGTC G[GA]^CG[CT]C
1150 GGCGCC
HinP1I 171 GCGC G^CGC
1151 GCGC
Ksp632I 415 CTCTTC CTCTTC(1/4)
608 GAAGAG
MaeII 431 ACGT A^CGT
491 ACGT
MflI 115 GGATCT [GA]^GATC[CT]
359 AGATCC
MluNI 482 TGGCCA TGG^CCA
803 TGGCCA
MscI 482 TGGCCA TGG^CCA
803 TGGCCA
MspA1I 858 CAGCGG C[AC]G^C[GT]G
1013 CAGCTG
NspI 549 GCATGC [GA]CATG^[CT]
670 ACATGC
PpuMI 281 AGGACCT [GA]G^G[AT]CC[CT]
1046 AGGTCCT
SbfI 561 CCTGCAGG CCTGCA^GG
1041 CCTGCAGG
Sse8387I 561 CCTGCAGG CCTGCA^GG
1041 CCTGCAGG
TfiI 586 GAATC G^A[AT]TC
864 GAATC
XhoII 115 GGATCT [GA]^GATC[CT]
359 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AluI AlwI AspHI AvaII BanII
BbsI BbvI BcnI BmyI BpuAI
BsaJI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeIII
MboI MboII MnlI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PstI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TseI
Tsp45I TspRI