################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## Acc65I AccII AccIII AclI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BbvI BciVI BfrI BglI BlnI BsaAI BsaI BseAI BseRI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I CfoI Cfr10I ClaI CpoI DraIII DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT22I FauI FseI FspI HaeII HhaI HinP1I KasI KpnI KspI MluI MroI MvnI MwoI NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SphI Sse8387I StuI SwaI TseI Tth111I Van91I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccI 3318 GTCTAC GT^[AC][GT]AC AciI 777 CCGC CCGC(-3/-1) AlwNI 3089 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 38 CCTCAGG CC^T[ACGT]AGG AseI 1825 ATTAAT AT^TAAT AsnI 1825 ATTAAT AT^TAAT AspHI 3537 GTGCTC G[AT]GC[AT]^C BalI 2370 TGGCCA TGG^CCA BcgI 1118 GCAAAATTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 2919 TGATCA T^GATCA BlpI 2686 GCTGAGC GC^T[ACGT]AGC BpmI 3266 CTCCAG CTGGAG(16/14) Bpu1102I 2686 GCTGAGC GC^T[ACGT]AGC BsaBI 3091 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 1331 GTGCAG GTGCAG(16/14) BsiHKAI 3537 GTGCTC G[AT]GC[AT]^C BsmFI 1753 GGGAC GGGAC(10/14) BsmI 2857 GAATGC GAATGC(1/-1) Bsp1407I 2496 TGTACA T^GTACA BspHI 338 TCATGA T^CATGA BsrGI 2496 TGTACA T^GTACA BstF5I 1317 GGATG GGATG(2/0) BstXI 765 CCAGTCTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 38 CCTCAGG CC^T[ACGT]AGG CelII 2686 GCTGAGC GC^T[ACGT]AGC DrdI 3225 GACGTCTAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 567 CTCTTC CTCTTC(1/4) Eco81I 38 CCTCAGG CC^T[ACGT]AGG EcoT14I 3530 CCTTGG C^C[AT][AT]GG FbaI 2919 TGATCA T^GATCA Fnu4HI 776 GCCGC GC^[ACGT]GC FokI 1317 GGATG GGATG(9/13) HgaI 456 GCGTC GACGC(5/10) HpaI 3024 GTTAAC GTT^AAC ItaI 776 GCCGC GC^[ACGT]GC Ksp632I 567 CTCTTC CTCTTC(1/4) MamI 3091 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1063 CAATTG C^AATTG MluNI 2370 TGGCCA TGG^CCA MscI 2370 TGGCCA TGG^CCA MunI 1063 CAATTG C^AATTG NheI 415 GCTAGC G^CTAGC PshBI 1825 ATTAAT AT^TAAT PstI 857 CTGCAG CTGCA^G RcaI 338 TCATGA T^CATGA SpeI 3079 ACTAGT A^CTAGT SspBI 2496 TGTACA T^GTACA SspI 535 AATATT AAT^ATT StyI 3530 CCTTGG C^C[AT][AT]GG XbaI 1600 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AatII 1687 GACGTC GACGT^C 3225 GACGTC AcyI 1687 GACGTC G[GA]^CG[CT]C 3225 GACGTC AflIII 1883 ACATGT A^C[GA][CT]GT 2793 ACATGT Asp700I 1185 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2449 GAAATAATTC AvaII 1754 GGACC G^G[AT]CC 2051 GGACC BcnI 1342 CCCGG CC^[GC]GG 2263 CCCGG BmyI 6 GTGCCC G[AGT]GC[ACT]^C 3537 GTGCTC BsaHI 1687 GACGTC G[GA]^CG[CT]C 3225 GACGTC BsaJI 2262 CCCCGG C^C[ACGT][ACGT]GG 3530 CCTTGG BsaWI 1850 ACCGGA [AT]^CCGG[AT] 3605 ACCGGA Bsp1286I 6 GTGCCC G[AGT]GC[ACT]^C 3537 GTGCTC BspLU11I 1883 ACATGT A^CATGT 2793 ACATGT BspMI 2298 ACCTGC ACCTGC(4/8) 2385 ACCTGC Cfr13I 1754 GGACC G^G[ACGT]CC 2051 GGACC DraII 1753 GGGACCC [GA]G^G[ACGT]CC[CT] 2050 AGGACCC EaeI 774 TGGCCG [CT]^GGCC[GA] 2370 TGGCCA EcoO109I 1753 GGGACCC [GA]G^G[ACGT]CC[CT] 2050 AGGACCC EcoRI 210 GAATTC G^AATTC 1569 GAATTC Hin1I 1687 GACGTC G[GA]^CG[CT]C 3225 GACGTC MspA1I 1366 CAGCTG C[AC]G^C[GT]G 1387 CAGCTG NciI 1342 CCCGG CC^[GC]GG 2263 CCCGG NspI 1883 ACATGT [GA]CATG^[CT] 2793 ACATGT PleI 3126 GACTC GAGTC(4/5) 1003 GAGTC PpuMI 1753 GGGACCC [GA]G^G[AT]CC[CT] 2050 AGGACCC PvuII 1366 CAGCTG CAG^CTG 1387 CAGCTG Sau96I 1754 GGACC G^G[ACGT]CC 2051 GGACC TaqI 216 TCGA T^CGA 1268 TCGA TthHB8I 216 TCGA T^CGA 1268 TCGA XmnI 1185 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2449 GAAATAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI BbsI BfaI BglII BpuAI BsiYI BslI BsmAI BsrI BssKI BstNI BstYI Cac8I DdeI DpnII DraI EcoRII HaeIII HapII HincII HindII HindIII HinfI HpaII HphI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI NdeII NlaIII NlaIV RsaI Sau3AI ScrFI SfaNI SfcI TfiI Tru9I Tsp45I Tsp509I TspRI XhoII