################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AgeI AhdI Alw44I ApaI ApaLI AscI Asp718I AspEI AspI AviII BbeI BbrPI BbvCI BcgI BclI BlpI Bpu1102I BsaAI BsaHI BseAI BsiEI BsiWI BsmFI BsmI Bsp1407I BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I CelII Cfr10I CpoI DrdI EagI Eam1105I EclXI Eco52I EcoO65I EcoRI FbaI FseI FspI HgaI Hin1I HincII HindII HpaI KasI KpnI KspI MfeI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI RcaI RsrII SacII SalI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI Tth111I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AflII 1036 CTTAAG C^TTAAG AflIII 1456 ACACGT A^C[GA][CT]GT AocI 1143 CCTTAGG CC^T[ACGT]AGG Aor51HI 801 AGCGCT AGC^GCT AseI 459 ATTAAT AT^TAAT AsnI 459 ATTAAT AT^TAAT Asp700I 837 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 556 CCTAGG C^CTAGG BalI 2 TGGCCA TGG^CCA BciVI 552 GTATCC GTATCC(6/5) BfrI 1036 CTTAAG C^TTAAG BglI 112 GCCCTGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 421 AGATCT A^GATCT BlnI 556 CCTAGG C^CTAGG BsaBI 422 GATCTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 923 GGTCTC GGTCTC(1/5) BsaWI 714 ACCGGA [AT]^CCGG[AT] BsgI 1502 GTGCAG GTGCAG(16/14) BsmBI 1975 GAGACG CGTCTC(1/5) BspDI 1058 ATCGAT AT^CGAT BspMI 1504 GCAGGT ACCTGC(4/8) Bsu36I 1143 CCTTAGG CC^T[ACGT]AGG ClaI 1058 ATCGAT AT^CGAT DraIII 13 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 1547 CCACGG C^C[GA][CT]GG EaeI 2 TGGCCA [CT]^GGCC[GA] Eco47III 801 AGCGCT AGC^GCT Eco81I 1143 CCTTAGG CC^T[ACGT]AGG EcoNI 481 CCTGATTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 781 GATATC GAT^ATC HaeII 801 AGCGCT [GA]GCGC^[CT] HindIII 2004 AAGCTT A^AGCTT MaeII 1458 ACGT A^CGT MamI 422 GATCTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 2 TGGCCA TGG^CCA MscI 2 TGGCCA TGG^CCA MslI 268 CACTCTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG PflMI 12 CCACCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 459 ATTAAT AT^TAAT SapI 1126 GAAGAGC GCTCTTC(1/4) SwaI 972 ATTTAAAT ATTT^AAAT Van91I 12 CCACCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 837 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccII 74 CGCG CG^CG 94 CGCG AlwNI 476 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG 857 CAGTTCCTG AspHI 589 GAGCTC G[AT]GC[AT]^C 1129 GAGCTC AvaI 1010 CCCGGG C^[CT]CG[GA]G 1682 CCCGGG AvaII 99 GGACC G^G[AT]CC 1296 GGACC BamHI 822 GGATCC G^GATCC 1628 GGATCC BanI 696 GGTGCC G^G[CT][GA]CC 1274 GGTGCC BsiHKAI 589 GAGCTC G[AT]GC[AT]^C 1129 GAGCTC BsoBI 1010 CCCGGG C^[CT]CG[GA]G 1682 CCCGGG BstUI 74 CGCG CG^CG 94 CGCG EcoT14I 556 CCTAGG C^C[AT][AT]GG 1477 CCAAGG EcoT22I 1379 ATGCAT ATGCA^T 1391 ATGCAT FauI 169 CCCGC CCCGC(4/6) 75 GCGGG MvnI 74 CGCG CG^CG 94 CGCG NsiI 1379 ATGCAT ATGCA^T 1391 ATGCAT NspI 219 ACATGC [GA]CATG^[CT] 1389 ACATGC PpuMI 98 AGGACCC [GA]G^G[AT]CC[CT] 1295 AGGACCT PvuII 662 CAGCTG CAG^CTG 1021 CAGCTG SacI 589 GAGCTC GAGCT^C 1129 GAGCTC ScaI 1950 AGTACT AGT^ACT 1958 AGTACT SmaI 1010 CCCGGG CCC^GGG 1682 CCCGGG StyI 556 CCTAGG C^C[AT][AT]GG 1477 CCAAGG TfiI 2080 GATTC G^A[AT]TC 2140 GAATC XmaCI 1010 CCCGGG C^CCGGG 1682 CCCGGG XmaI 1010 CCCGGG C^CCGGG 1682 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 76 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EarI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII