Restriction map for
pF1KE0324
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaII AviII AvrII BbeI
BbvCI BcgI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsmFI BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstPI
BstUI BstXI Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
FauI FseI FspI HaeII HhaI
Hin1I HinP1I KasI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I StuI SwaI Tth111I XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 411 GTATAC GT^[AC][GT]AC
ApaI 511 GGGCCC GGGCC^C
AvaI 104 CCCGGG C^[CT]CG[GA]G
BalI 1727 TGGCCA TGG^CCA
BamHI 17 GGATCC G^GATCC
BanI 1127 GGTGCC G^G[CT][GA]CC
BbrPI 1741 CACGTG CAC^GTG
BbsI 48 GAAGAC GAAGAC(2/6)
BciVI 1734 GTATCC GTATCC(6/5)
BclI 588 TGATCA T^GATCA
BpmI 676 CTGGAG CTGGAG(16/14)
BpuAI 48 GAAGAC GAAGAC(2/6)
BsaAI 1741 CACGTG [CT]AC^GT[GA]
BsaI 385 GAGACC GGTCTC(1/5)
BsmI 261 GCATTC GAATGC(1/-1)
BsoBI 104 CCCGGG C^[CT]CG[GA]G
Bst1107I 411 GTATAC GTA^TAC
BstYI 17 GGATCC [GA]^GATC[CT]
BstZ17I 411 GTATAC GTA^TAC
Cac8I 326 GCAGGC GC[ACGT]^[ACGT]GC
EaeI 1727 TGGCCA [CT]^GGCC[GA]
EcoT14I 1018 CCTTGG C^C[AT][AT]GG
EcoT22I 911 ATGCAT ATGCA^T
FbaI 588 TGATCA T^GATCA
HapII 105 CCGG C^CGG
HgaI 1098 GACGC GACGC(5/10)
HindIII 1184 AAGCTT A^AGCTT
HpaI 250 GTTAAC GTT^AAC
HpaII 105 CCGG C^CGG
MflI 17 GGATCC [GA]^GATC[CT]
MluNI 1727 TGGCCA TGG^CCA
MscI 1727 TGGCCA TGG^CCA
MslI 706 CATATAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 105 CCGG C^CGG
NsiI 911 ATGCAT ATGCA^T
PflMI 1769 CCACGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1177 GAGTC GAGTC(4/5)
PmaCI 1741 CACGTG CAC^GTG
PmlI 1741 CACGTG CAC^GTG
SapI 1309 GCTCTTC GCTCTTC(1/4)
SmaI 104 CCCGGG CCC^GGG
StyI 1018 CCTTGG C^C[AT][AT]GG
Van91I 1769 CCACGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 17 GGATCC [GA]^GATC[CT]
XmaCI 104 CCCGGG C^CCGGG
XmaI 104 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AlwI 18 GATCC GGATC(4/5)
17 GGATC
AspHI 469 GAGCTC G[AT]GC[AT]^C
1764 GAGCTC
BbvI 330 GCTGC GCAGC(8/12)
1576 GCTGC
BcnI 104 CCCGG CC^[GC]GG
105 CCGGG
BfaI 38 CTAG C^TAG
1150 CTAG
BseRI 665 CTCCTC GAGGAG(10/8)
529 GAGGAG
BsiHKAI 469 GAGCTC G[AT]GC[AT]^C
1764 GAGCTC
Bsp1407I 1119 TGTACA T^GTACA
1829 TGTACA
BspMI 1223 ACCTGC ACCTGC(4/8)
1589 ACCTGC
BsrGI 1119 TGTACA T^GTACA
1829 TGTACA
DraI 577 TTTAAA TTT^AAA
997 TTTAAA
DraII 510 AGGGCCC [GA]G^G[ACGT]CC[CT]
511 GGGCCCC
EarI 1310 CTCTTC CTCTTC(1/4)
91 GAAGAG
EcoO109I 510 AGGGCCC [GA]G^G[ACGT]CC[CT]
511 GGGCCCC
EcoRV 1423 GATATC GAT^ATC
1702 GATATC
Fnu4HI 330 GCTGC GC^[ACGT]GC
1576 GCTGC
HincII 250 GTTAAC GT[CT]^[GA]AC
735 GTTGAC
HindII 250 GTTAAC GT[CT]^[GA]AC
735 GTTGAC
ItaI 330 GCTGC GC^[ACGT]GC
1576 GCTGC
Ksp632I 1310 CTCTTC CTCTTC(1/4)
91 GAAGAG
MaeI 38 CTAG C^TAG
1150 CTAG
NciI 104 CCCGG CC^[GC]GG
105 CCGGG
PstI 1225 CTGCAG CTGCA^G
1635 CTGCAG
PvuII 128 CAGCTG CAG^CTG
1190 CAGCTG
SacI 469 GAGCTC GAGCT^C
1764 GAGCTC
SfaNI 616 GATGC GCATC(5/9)
1687 GATGC
SspBI 1119 TGTACA T^GTACA
1829 TGTACA
TseI 330 GCTGC G^C[AT]GC
1576 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
Asp700I BanII BmyI BsaJI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI Cfr13I DdeI DpnII
EcoRII FokI HaeIII HinfI HphI
MaeII MaeIII MboI MboII MnlI
MseI MspA1I MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfcI SmlI SspI TaqI
TfiI Tru9I Tsp45I Tsp509I TspRI
TthHB8I XmnI