Restriction map for
pF1KE0331
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# NO CUT ENZYMES ( 142 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BclI BcnI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmI BsoBI BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraIII DrdI DsaI EaeI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoT22I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HpaI HpaII
KasI KpnI KspI MaeII MluI
MluNI MroI MscI MslI MspI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI Psp1406I PstI PvuI
RsrII SacI SacII SalI SapI
SbfI SexAI SfcI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StuI SwaI XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 65 enzymes) #
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AccI 1666 GTAGAC GT^[AC][GT]AC
AhdI 48 GACAAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 984 ATTAAT AT^TAAT
AsnI 984 ATTAAT AT^TAAT
Asp700I 1371 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 48 GACAAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 895 GAGCAC G[AT]GC[AT]^C
AspI 867 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 154 GGACC G^G[AT]CC
BbvI 1948 GCTGC GCAGC(8/12)
BcgI 1674 CGAAGGGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 969 GGATAC GTATCC(6/5)
BglII 1702 AGATCT A^GATCT
BmyI 895 GAGCAC G[AGT]GC[ACT]^C
BsaBI 1507 GATAATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1572 GGTCTC GGTCTC(1/5)
BseRI 689 GAGGAG GAGGAG(10/8)
BsiHKAI 895 GAGCAC G[AT]GC[AT]^C
BsmFI 640 GTCCC GGGAC(10/14)
Bsp1286I 895 GAGCAC G[AGT]GC[ACT]^C
BspHI 1844 TCATGA T^CATGA
BssKI 487 CCTGG ^CC[ACGT]GG
BstBI 1653 TTCGAA TT^CGAA
BstF5I 1237 CATCC GGATG(2/0)
BstNI 487 CCTGG CC^[AT]GG
BstYI 1702 AGATCT [GA]^GATC[CT]
DraI 1900 TTTAAA TTT^AAA
Eam1105I 48 GACAAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRII 487 CCTGG ^CC[AT]GG
Fnu4HI 1948 GCTGC GC^[ACGT]GC
FokI 1237 CATCC GGATG(9/13)
HaeIII 1679 GGCC GG^CC
HincII 327 GTCAAC GT[CT]^[GA]AC
HindII 327 GTCAAC GT[CT]^[GA]AC
HindIII 113 AAGCTT A^AGCTT
ItaI 1948 GCTGC GC^[ACGT]GC
MamI 1507 GATAATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1865 CAATTG C^AATTG
MflI 1702 AGATCT [GA]^GATC[CT]
MspA1I 653 CAGCTG C[AC]G^C[GT]G
MunI 1865 CAATTG C^AATTG
MvaI 487 CCTGG CC^[AT]GG
MwoI 538 GCATTAGAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 1163 CATATG CA^TATG
NlaIV 546 GGCTCC GG[ACGT]^[ACGT]CC
NspI 636 GCATGT [GA]CATG^[CT]
NspV 1653 TTCGAA TT^CGAA
PflFI 867 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 917 CCACTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 153 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 984 ATTAAT AT^TAAT
PvuII 653 CAGCTG CAG^CTG
RcaI 1844 TCATGA T^CATGA
ScaI 511 AGTACT AGT^ACT
ScrFI 487 CCTGG CC^[ACGT]GG
SfuI 1653 TTCGAA TT^CGAA
SspI 1071 AATATT AAT^ATT
TaqI 1654 TCGA T^CGA
TseI 1948 GCTGC G^C[AT]GC
Tth111I 867 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1654 TCGA T^CGA
Van91I 917 CCACTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1445 CCAAACAGATACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1702 AGATCT [GA]^GATC[CT]
XmnI 1371 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
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AlwI 618 GGATC GGATC(4/5)
1458 GGATC
AlwNI 1450 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
1804 CAGATTCTG
BsiYI 27 CCTTCTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
917 CCACTGGTTGG
BslI 27 CCTTCTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
917 CCACTGGTTGG
Bsp1407I 735 TGTACA T^GTACA
1063 TGTACA
BsrGI 735 TGTACA T^GTACA
1063 TGTACA
Cac8I 75 GCTGGC GC[ACGT]^[ACGT]GC
1680 GCCTGC
Cfr13I 154 GGACC G^G[ACGT]CC
1678 GGGCC
DraII 153 AGGACCT [GA]G^G[ACGT]CC[CT]
1677 AGGGCCT
EcoO109I 153 AGGACCT [GA]G^G[ACGT]CC[CT]
1677 AGGGCCT
EcoRI 1598 GAATTC G^AATTC
1848 GAATTC
EcoRV 195 GATATC GAT^ATC
556 GATATC
Sau96I 154 GGACC G^G[ACGT]CC
1678 GGGCC
SmlI 1110 CTTGAG C^T[CT][GA]AG
1117 CTCAAG
SspBI 735 TGTACA T^GTACA
1063 TGTACA
Tsp45I 98 GTGAC ^GT[GC]AC
404 GTGAC
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbsI
BfaI BpmI BpuAI BsaJI BsmAI
BsrI DdeI DpnII EarI EcoT14I
HinfI HphI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI NdeII
NlaIII PleI RsaI Sau3AI SfaNI
StyI TfiI Tru9I Tsp509I TspRI