Restriction map for
pF1KE0372
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# NO CUT ENZYMES ( 144 enzymes) #
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Acc65I AccII AccIII AclI AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AvaI
AviII AvrII BamHI BanI BbeI
BbrPI BbvCI BbvI BcgI BciVI
BcnI BfaI BglI BlnI BlpI
Bpu1102I BsaBI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmFI BsoBI
Bsp1407I BspDI BspEI BspHI BspMI
BsrBI BsrFI BsrGI BsrI BssHII
BssSI BstBI BstEII BstPI BstUI
BstXI CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I Fnu4HI FseI FspI HaeII
HapII HgaI HhaI HinP1I HpaII
ItaI KasI KpnI KspI MaeI
MamI MluI MroI MspI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI TaqI TseI TthHB8I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 1387 GACGTC GACGT^C
AciI 603 GCGG CCGC(-3/-1)
AcyI 1387 GACGTC G[GA]^CG[CT]C
AflII 1392 CTTAAG C^TTAAG
AlwI 1503 GGATC GGATC(4/5)
AlwNI 122 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1109 CCTTAGG CC^T[ACGT]AGG
AseI 849 ATTAAT AT^TAAT
AsnI 849 ATTAAT AT^TAAT
AspHI 75 GAGCTC G[AT]GC[AT]^C
AspI 1300 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BfrI 1392 CTTAAG C^TTAAG
BglII 552 AGATCT A^GATCT
BsaAI 473 CACGTA [CT]AC^GT[GA]
BsaHI 1387 GACGTC G[GA]^CG[CT]C
BsaI 369 GGTCTC GGTCTC(1/5)
BsiHKAI 75 GAGCTC G[AT]GC[AT]^C
BsmI 1171 GAATGC GAATGC(1/-1)
BspLU11I 1633 ACATGT A^CATGT
Bst1107I 1214 GTATAC GTA^TAC
BstAPI 883 GCAGAAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 552 AGATCT [GA]^GATC[CT]
BstZ17I 1214 GTATAC GTA^TAC
Bsu36I 1109 CCTTAGG CC^T[ACGT]AGG
DrdI 1381 GACAAAGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 968 CTCTTC CTCTTC(1/4)
Eco81I 1109 CCTTAGG CC^T[ACGT]AGG
FauI 603 GCGGG CCCGC(4/6)
Hin1I 1387 GACGTC G[GA]^CG[CT]C
HincII 1639 GTTAAC GT[CT]^[GA]AC
HindII 1639 GTTAAC GT[CT]^[GA]AC
HindIII 1049 AAGCTT A^AGCTT
HpaI 1639 GTTAAC GTT^AAC
Ksp632I 968 CTCTTC CTCTTC(1/4)
MflI 552 AGATCT [GA]^GATC[CT]
MspA1I 374 CAGCTG C[AC]G^C[GT]G
NlaIV 1315 GGAACC GG[ACGT]^[ACGT]CC
NspI 1633 ACATGT [GA]CATG^[CT]
PflFI 1300 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1494 CCACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 849 ATTAAT AT^TAAT
PstI 881 CTGCAG CTGCA^G
PvuII 374 CAGCTG CAG^CTG
SacI 75 GAGCTC GAGCT^C
Tsp45I 803 GTGAC ^GT[GC]AC
Tth111I 1300 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1494 CCACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 552 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 188 GTAC GT^AC
618 GTAC
AflIII 472 ACACGT A^C[GA][CT]GT
1633 ACATGT
AvaII 308 GGACC G^G[AT]CC
1515 GGTCC
BalI 744 TGGCCA TGG^CCA
1275 TGGCCA
BanII 75 GAGCTC G[GA]GC[CT]^C
465 GGGCTC
BbsI 790 GAAGAC GAAGAC(2/6)
10 GTCTTC
BclI 513 TGATCA T^GATCA
854 TGATCA
BpuAI 790 GAAGAC GAAGAC(2/6)
10 GTCTTC
BsaJI 68 CCTTGG C^C[ACGT][ACGT]GG
160 CCTTGG
BsrDI 99 CATTGC GCAATG(2/0)
1083 CATTGC
EcoT14I 68 CCTTGG C^C[AT][AT]GG
160 CCTTGG
FbaI 513 TGATCA T^GATCA
854 TGATCA
MfeI 287 CAATTG C^AATTG
1295 CAATTG
MluNI 744 TGGCCA TGG^CCA
1275 TGGCCA
MscI 744 TGGCCA TGG^CCA
1275 TGGCCA
MunI 287 CAATTG C^AATTG
1295 CAATTG
RsaI 188 GTAC GT^AC
618 GTAC
SfcI 645 CTGTAG C^T[GA][CT]AG
881 CTGCAG
SmlI 634 CTTGAG C^T[CT][GA]AG
1392 CTTAAG
SspI 673 AATATT AAT^ATT
977 AATATT
StyI 68 CCTTGG C^C[AT][AT]GG
160 CCTTGG
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AccI AcsI AluI ApoI BmyI
BpmI BseRI BsiYI BslI BsmAI
Bsp1286I BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EaeI EcoRII
FokI HaeIII HinfI HphI MaeII
MaeIII MboI MboII MnlI MseI
MslI MvaI MwoI NdeII NlaIII
PleI Sau3AI Sau96I ScrFI SfaNI
TfiI Tru9I Tsp509I TspRI XcmI