Restriction map for
pF1KE0383
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI Alw44I ApaI ApaLI
AscI Asp700I Asp718I AvaI AviII
AvrII BamHI BbeI BbvCI BcgI
BciVI BfrI BlnI BsaBI BsaHI
BseAI BsgI BsiWI BsmBI BsoBI
BspDI BspEI BsrBI BsrFI BssHII
Bst1107I BstEII BstPI BstZ17I Cfr10I
ClaI CpoI DraI DraIII EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
Hin1I HincII HindII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI NaeI NarI NgoMIV
NheI NruI NsiI PacI PaeR7I
PinAI PmeI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SwaI XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 599 GTAGAC GT^[AC][GT]AC
AhdI 805 GACCTCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2214 CCTAAGG CC^T[ACGT]AGG
Aor51HI 707 AGCGCT AGC^GCT
AseI 3136 ATTAAT AT^TAAT
AsnI 3136 ATTAAT AT^TAAT
AspEI 805 GACCTCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 1603 CACGTG CAC^GTG
BclI 992 TGATCA T^GATCA
BglI 235 GCCAGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1416 AGATCT A^GATCT
BsaAI 1603 CACGTG [CT]AC^GT[GA]
BsaI 1127 GAGACC GGTCTC(1/5)
BsaWI 1283 TCCGGT [AT]^CCGG[AT]
BspLU11I 2301 ACATGT A^CATGT
BspMI 1489 GCAGGT ACCTGC(4/8)
BstAPI 921 GCAGAAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 303 TTCGAA TT^CGAA
BstXI 608 CCAAGACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2214 CCTAAGG CC^T[ACGT]AGG
DrdI 1501 GACCACTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 805 GACCTCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 707 AGCGCT AGC^GCT
Eco81I 2214 CCTAAGG CC^T[ACGT]AGG
EcoNI 2582 CCTGTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 1818 CCCGC CCCGC(4/6)
FbaI 992 TGATCA T^GATCA
HgaI 284 GACGC GACGC(5/10)
HindIII 2329 AAGCTT A^AGCTT
NdeI 489 CATATG CA^TATG
NotI 2170 GCGGCCGC GC^GGCCGC
NspV 303 TTCGAA TT^CGAA
PmaCI 1603 CACGTG CAC^GTG
PmlI 1603 CACGTG CAC^GTG
PshAI 1443 GACGGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3136 ATTAAT AT^TAAT
SexAI 221 ACCAGGT A^CC[AT]GGT
SfuI 303 TTCGAA TT^CGAA
SspI 1958 AATATT AAT^ATT
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 286 CGCG CG^CG
2169 CGCG
AflIII 2003 ACGTGT A^C[GA][CT]GT
2301 ACATGT
AspI 220 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1129 GACCAAGTC
BlpI 957 GCTGAGC GC^T[ACGT]AGC
2013 GCTCAGC
Bpu1102I 957 GCTGAGC GC^T[ACGT]AGC
2013 GCTCAGC
BsiEI 1706 CGGCCG CG[GA][CT]^CG
2171 CGGCCG
BsmI 2664 GAATGC GAATGC(1/-1)
2970 GAATGC
BspHI 995 TCATGA T^CATGA
1997 TCATGA
BsrDI 730 CATTGC GCAATG(2/0)
2054 GCAATG
BstUI 286 CGCG CG^CG
2169 CGCG
CelII 957 GCTGAGC GC^T[ACGT]AGC
2013 GCTCAGC
EagI 1706 CGGCCG C^GGCCG
2171 CGGCCG
EclXI 1706 CGGCCG C^GGCCG
2171 CGGCCG
Eco52I 1706 CGGCCG C^GGCCG
2171 CGGCCG
MspA1I 237 CAGCTG C[AC]G^C[GT]G
2518 CAGCTG
MvnI 286 CGCG CG^CG
2169 CGCG
NcoI 1148 CCATGG C^CATGG
2915 CCATGG
PflFI 220 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1129 GACCAAGTC
PflMI 209 CCATCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2910 CCAGGCCATGG
PstI 2368 CTGCAG CTGCA^G
2692 CTGCAG
PvuII 237 CAGCTG CAG^CTG
2518 CAGCTG
RcaI 995 TCATGA T^CATGA
1997 TCATGA
ScaI 193 AGTACT AGT^ACT
560 AGTACT
SfcI 2368 CTGCAG C^T[GA][CT]AG
2692 CTGCAG
StuI 1694 AGGCCT AGG^CCT
3008 AGGCCT
Tth111I 220 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1129 GACCAAGTC
Van91I 209 CCATCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2910 CCAGGCCATGG
XcmI 1098 CCAGATGTATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2132 CCAACAGTGTTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BsrGI BsrI
BssKI BssSI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII