Restriction map for
pF1KE0388
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaII
AviII AvrII BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaHI BsaI BsaWI BseAI
BsiHKAI BsiWI BsmBI BsmI BspDI
BspEI BspLU11I BspMI BsrBI BssHII
BssSI Bst1107I BstAPI BstBI BstZ17I
CelII ClaI CpoI DraIII DrdI
Eam1105I EarI EcoRI EcoRV EcoT22I
FbaI FseI FspI Hin1I HpaI
KasI KpnI Ksp632I MamI MfeI
MroI MslI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacI
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AflIII 676 ACGCGT A^C[GA][CT]GT
Aor51HI 1570 AGCGCT AGC^GCT
AvaI 45 CCCGGG C^[CT]CG[GA]G
BalI 1376 TGGCCA TGG^CCA
BbsI 1169 GTCTTC GAAGAC(2/6)
BglI 1813 GCCCTGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 1467 CTCCAG CTGGAG(16/14)
BpuAI 1169 GTCTTC GAAGAC(2/6)
BsaAI 1636 TACGTG [CT]AC^GT[GA]
BseRI 1759 GAGGAG GAGGAG(10/8)
BsiEI 25 CGGCCG CG[GA][CT]^CG
BsmFI 1505 GGGAC GGGAC(10/14)
BsoBI 45 CCCGGG C^[CT]CG[GA]G
Bsp1407I 651 TGTACA T^GTACA
BspHI 1613 TCATGA T^CATGA
BsrDI 394 GCAATG GCAATG(2/0)
BsrFI 221 GCCGGC [GA]^CCGG[CT]
BsrGI 651 TGTACA T^GTACA
BstEII 1681 GGTTACC G^GT[ACGT]ACC
BstPI 1681 GGTTACC G^GT[ACGT]ACC
BstXI 1522 CCAGTTCGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 221 GCCGGC [GA]^CCGG[CT]
DraI 562 TTTAAA TTT^AAA
EagI 25 CGGCCG C^GGCCG
EclXI 25 CGGCCG C^GGCCG
Eco47III 1570 AGCGCT AGC^GCT
Eco52I 25 CGGCCG C^GGCCG
EcoNI 201 CCTGAGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1681 GGTTACC G^GT[ACGT]ACC
HgaI 1599 GACGC GACGC(5/10)
HincII 412 GTCAAC GT[CT]^[GA]AC
HindII 412 GTCAAC GT[CT]^[GA]AC
HindIII 1115 AAGCTT A^AGCTT
MluI 676 ACGCGT A^CGCGT
MluNI 1376 TGGCCA TGG^CCA
MscI 1376 TGGCCA TGG^CCA
NaeI 221 GCCGGC GCC^GGC
NgoMIV 221 GCCGGC G^CCGGC
PstI 163 CTGCAG CTGCA^G
PvuII 1780 CAGCTG CAG^CTG
RcaI 1613 TCATGA T^CATGA
SexAI 1331 ACCTGGT A^CC[AT]GGT
SfcI 163 CTGCAG C^T[GA][CT]AG
SmaI 45 CCCGGG CCC^GGG
SmlI 930 CTTGAG C^T[CT][GA]AG
SphI 339 GCATGC GCATG^C
SspBI 651 TGTACA T^GTACA
TaqI 1655 TCGA T^CGA
TthHB8I 1655 TCGA T^CGA
XmaCI 45 CCCGGG C^CCGGG
XmaI 45 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AocI 60 CCTGAGG CC^T[ACGT]AGG
893 CCTTAGG
BsgI 325 CTGCAC GTGCAG(16/14)
710 GTGCAG
BstYI 1068 AGATCC [GA]^GATC[CT]
1752 AGATCC
Bsu36I 60 CCTGAGG CC^T[ACGT]AGG
893 CCTTAGG
Eco81I 60 CCTGAGG CC^T[ACGT]AGG
893 CCTTAGG
HaeII 127 GGCGCT [GA]GCGC^[CT]
1570 AGCGCT
MaeII 1637 ACGT A^CGT
1826 ACGT
MflI 1068 AGATCC [GA]^GATC[CT]
1752 AGATCC
NspI 339 GCATGC [GA]CATG^[CT]
654 ACATGC
SfaNI 238 GATGC GCATC(5/9)
1140 GATGC
SspI 717 AATATT AAT^ATT
735 AATATT
XhoII 1068 AGATCC [GA]^GATC[CT]
1752 AGATCC
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# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI BanII
BbvI BcnI BfaI BmyI BsaJI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI KspI MaeI MaeIII MboI
MboII MnlI MseI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PleI RsaI
SacII Sau3AI Sau96I ScrFI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI