Restriction map for
pF1KE0390
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AccIII AclI AflII AgeI Aor51HI
AscI AseI AsnI Asp700I AspI
AvrII BamHI BbeI BciVI BclI
BfaI BfrI BlnI BlpI Bpu1102I
BsaBI BsaWI BseAI BsiEI BsiWI
BsmI BspDI BspEI BspHI BspLU11I
BsrDI BssHII Bst1107I BstBI BstEII
BstPI BstXI BstZ17I CelII ClaI
CpoI DraI DrdI EagI EclXI
Eco47III Eco52I EcoO65I EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HinfI HpaI KasI MaeI MamI
MfeI MluI MroI MseI MunI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SspI SwaI TfiI Tru9I Tth111I
Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 1222 GACGTC GACGT^C
Acc65I 1325 GGTACC G^GTACC
AccI 1363 GTCTAC GT^[AC][GT]AC
AcsI 685 GAATTC [GA]^AATT[CT]
AflIII 662 ACGTGT A^C[GA][CT]GT
AhdI 1222 GACGTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1039 GTGCAC G^TGCAC
ApaLI 1039 GTGCAC G^TGCAC
ApoI 685 GAATTC [GA]^AATT[CT]
Asp718I 1325 GGTACC G^GTACC
AspEI 1222 GACGTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 914 TGCGCA TGC^GCA
BbrPI 316 CACGTG CAC^GTG
BbsI 1204 GTCTTC GAAGAC(2/6)
BcgI 868 GCAGTGTGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 53 AGATCT A^GATCT
BpuAI 1204 GTCTTC GAAGAC(2/6)
BsaI 510 GGTCTC GGTCTC(1/5)
Bsp1407I 893 TGTACA T^GTACA
BsrGI 893 TGTACA T^GTACA
BstAPI 1311 GCAGATCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 865 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1222 GACGTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 685 GAATTC G^AATTC
FspI 914 TGCGCA TGC^GCA
KpnI 1325 GGTACC GGTAC^C
KspI 219 CCGCGG CCGC^GG
MaeIII 901 GTCAC ^GT[ACGT]AC
MslI 312 CACCCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 60 GCCGGC GCC^GGC
NgoMIV 60 GCCGGC G^CCGGC
NspI 635 GCATGC [GA]CATG^[CT]
PmaCI 316 CACGTG CAC^GTG
PmlI 316 CACGTG CAC^GTG
PpuMI 94 GGGTCCC [GA]G^G[AT]CC[CT]
SacII 219 CCGCGG CCGC^GG
SapI 162 GCTCTTC GCTCTTC(1/4)
SbfI 927 CCTGCAGG CCTGCA^GG
SfaNI 1454 GATGC GCATC(5/9)
SmaI 91 CCCGGG CCC^GGG
SmlI 1123 CTCAAG C^T[CT][GA]AG
SphI 635 GCATGC GCATG^C
Sse8387I 927 CCTGCAGG CCTGCA^GG
SspBI 893 TGTACA T^GTACA
TaqI 242 TCGA T^CGA
Tsp45I 901 GTCAC ^GT[GC]AC
TthHB8I 242 TCGA T^CGA
XcmI 308 CCATCACCCACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 91 CCCGGG C^CCGGG
XmaI 91 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 1222 GACGTC G[GA]^CG[CT]C
1237 GGCGTC
AocI 12 CCTCAGG CC^T[ACGT]AGG
1183 CCTGAGG
ApaI 482 GGGCCC GGGCC^C
959 GGGCCC
BalI 320 TGGCCA TGG^CCA
1266 TGGCCA
BbvCI 157 CCTCAGC CCTCAGC(-5/-2)
1003 GCTGAGG
BglI 455 GCCTCGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1348 GCCTGCCCGGC
BsaAI 316 CACGTG [CT]AC^GT[GA]
1366 TACGTG
BsaHI 1222 GACGTC G[GA]^CG[CT]C
1237 GGCGTC
BsmBI 337 GAGACG CGTCTC(1/5)
1300 GAGACG
BspMI 539 ACCTGC ACCTGC(4/8)
926 ACCTGC
BsrBI 670 GAGCGG CCGCTC(-3/-3)
847 GAGCGG
BsrFI 60 GCCGGC [GA]^CCGG[CT]
1328 ACCGGC
Bsu36I 12 CCTCAGG CC^T[ACGT]AGG
1183 CCTGAGG
Cfr10I 60 GCCGGC [GA]^CCGG[CT]
1328 ACCGGC
EarI 163 CTCTTC CTCTTC(1/4)
244 GAAGAG
Eco81I 12 CCTCAGG CC^T[ACGT]AGG
1183 CCTGAGG
HaeII 292 AGCGCC [GA]GCGC^[CT]
1179 AGCGCC
Hin1I 1222 GACGTC G[GA]^CG[CT]C
1237 GGCGTC
Ksp632I 163 CTCTTC CTCTTC(1/4)
244 GAAGAG
MluNI 320 TGGCCA TGG^CCA
1266 TGGCCA
MscI 320 TGGCCA TGG^CCA
1266 TGGCCA
NcoI 81 CCATGG C^CATGG
515 CCATGG
PvuII 394 CAGCTG CAG^CTG
521 CAGCTG
StuI 738 AGGCCT AGG^CCT
932 AGGCCT
Tsp509I 686 AATT ^AATT
1195 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BanI
BanII BbvI BcnI BmyI BpmI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BssSI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HpaII HphI ItaI
MaeII MboI MboII MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PstI
RsaI Sau3AI Sau96I ScrFI SfcI
StyI TseI TspRI XhoII