Restriction map for
pF1KE1398
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
Alw44I ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BamHI BbeI BbrPI BglI
BlnI BlpI Bpu1102I BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI BspDI BspEI BspMI BsrFI
BssHII BssSI Bst1107I BstAPI BstZ17I
CelII Cfr10I ClaI CpoI DraIII
DrdI EagI EclXI Eco52I EcoT22I
FseI FspI Hin1I HindIII KasI
KpnI MluI MroI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
PvuI PvuII RsrII SalI SapI
SbfI ScaI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AclI 3003 AACGTT AA^CGTT
AflII 2641 CTTAAG C^TTAAG
AlwNI 3073 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1324 CCTAAGG CC^T[ACGT]AGG
Aor51HI 227 AGCGCT AGC^GCT
BalI 1728 TGGCCA TGG^CCA
BcgI 2001 CGAGCTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3029 GGATAC GTATCC(6/5)
BclI 122 TGATCA T^GATCA
BfrI 2641 CTTAAG C^TTAAG
BsaAI 2138 TACGTG [CT]AC^GT[GA]
BspHI 2046 TCATGA T^CATGA
BsrBI 1669 GAGCGG CCGCTC(-3/-3)
BstBI 2909 TTCGAA TT^CGAA
BstEII 1590 GGTCACC G^GT[ACGT]ACC
BstPI 1590 GGTCACC G^GT[ACGT]ACC
Bsu36I 1324 CCTAAGG CC^T[ACGT]AGG
EaeI 1728 TGGCCA [CT]^GGCC[GA]
Eco47III 227 AGCGCT AGC^GCT
Eco81I 1324 CCTAAGG CC^T[ACGT]AGG
EcoO65I 1590 GGTCACC G^GT[ACGT]ACC
EcoRV 1045 GATATC GAT^ATC
FbaI 122 TGATCA T^GATCA
HincII 834 GTTAAC GT[CT]^[GA]AC
HindII 834 GTTAAC GT[CT]^[GA]AC
HpaI 834 GTTAAC GTT^AAC
MluNI 1728 TGGCCA TGG^CCA
MscI 1728 TGGCCA TGG^CCA
NspV 2909 TTCGAA TT^CGAA
PflMI 380 CCACGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 3003 AACGTT AA^CGTT
PstI 2509 CTGCAG CTGCA^G
RcaI 2046 TCATGA T^CATGA
SacI 1948 GAGCTC GAGCT^C
SexAI 2933 ACCAGGT A^CC[AT]GGT
SfuI 2909 TTCGAA TT^CGAA
SspI 1977 AATATT AAT^ATT
Van91I 380 CCACGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccI 1009 GTAGAC GT^[AC][GT]AC
1549 GTCTAC
AspHI 1483 GAGCAC G[AT]GC[AT]^C
1948 GAGCTC
AvaI 58 CCCGAG C^[CT]CG[GA]G
1999 CCCGAG
BanI 937 GGCACC G^G[CT][GA]CC
952 GGCACC
BbsI 3157 GAAGAC GAAGAC(2/6)
2995 GTCTTC
BbvCI 1609 CCTCAGC CCTCAGC(-5/-2)
3024 GCTGAGG
BcnI 2754 CCGGG CC^[GC]GG
3199 CCCGG
BglII 2364 AGATCT A^GATCT
2586 AGATCT
BpmI 355 CTCCAG CTGGAG(16/14)
516 CTGGAG
BpuAI 3157 GAAGAC GAAGAC(2/6)
2995 GTCTTC
BsaBI 2038 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2646 GATGAAAATC
BsgI 1527 CTGCAC GTGCAG(16/14)
1699 GTGCAG
BsiHKAI 1483 GAGCAC G[AT]GC[AT]^C
1948 GAGCTC
BsoBI 58 CCCGAG C^[CT]CG[GA]G
1999 CCCGAG
Bsp1407I 582 TGTACA T^GTACA
725 TGTACA
BspLU11I 1541 ACATGT A^CATGT
1745 ACATGT
BsrGI 582 TGTACA T^GTACA
725 TGTACA
BstXI 503 CCAATTTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1719 CCAGTTTTGTGG
DraI 2680 TTTAAA TTT^AAA
3112 TTTAAA
DraII 1328 AGGACCC [GA]G^G[ACGT]CC[CT]
2756 GGGACCT
EcoO109I 1328 AGGACCC [GA]G^G[ACGT]CC[CT]
2756 GGGACCT
EcoRI 1276 GAATTC G^AATTC
2234 GAATTC
FauI 34 GCGGG CCCGC(4/6)
79 GCGGG
HaeII 194 GGCGCT [GA]GCGC^[CT]
227 AGCGCT
HgaI 1138 GACGC GACGC(5/10)
764 GCGTC
KspI 161 CCGCGG CCGC^GG
972 CCGCGG
MamI 2038 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2646 GATGAAAATC
MfeI 1478 CAATTG C^AATTG
3122 CAATTG
MspA1I 161 CCGCGG C[AC]G^C[GT]G
972 CCGCGG
MunI 1478 CAATTG C^AATTG
3122 CAATTG
NciI 2754 CCGGG CC^[GC]GG
3199 CCCGG
NspI 1541 ACATGT [GA]CATG^[CT]
1745 ACATGT
PleI 247 GAGTC GAGTC(4/5)
3264 GAGTC
PpuMI 1328 AGGACCC [GA]G^G[AT]CC[CT]
2756 GGGACCT
SacII 161 CCGCGG CCGC^GG
972 CCGCGG
SmlI 2525 CTCAAG C^T[CT][GA]AG
2641 CTTAAG
SspBI 582 TGTACA T^GTACA
725 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AhdI AluI AlwI ApoI AspEI
AvaII BanII BbvI BfaI BmyI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
Eam1105I EarI EcoNI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MvnI MwoI NcoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII