Restriction map for
pF1KB0452
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# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AclI AgeI AhdI Alw44I
ApaLI AscI AseI AsnI AspEI
AviII BalI BamHI BclI BglII
BlpI Bpu1102I BsaBI BsaI BsiWI
BsmBI BspDI BspHI BsrBI BsrFI
BssHII BssSI Bst1107I BstZ17I CelII
Cfr10I ClaI DraI DrdI Eam1105I
EcoRV EcoT22I FbaI FseI FspI
HpaI KspI MamI MfeI MluI
MluNI MscI MunI NaeI NdeI
NgoMIV NheI NotI NsiI PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I RcaI SacII SalI SapI
SbfI SfiI SgrAI SnaBI SpeI
Sse8387I SspI SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 58 enzymes) #
###################################
Acc65I 1505 GGTACC G^GTACC
AcyI 151 GGCGCC G[GA]^CG[CT]C
AflII 513 CTTAAG C^TTAAG
AocI 1456 CCTCAGG CC^T[ACGT]AGG
Aor51HI 982 AGCGCT AGC^GCT
Asp718I 1505 GGTACC G^GTACC
AspI 1977 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 966 CCTAGG C^CTAGG
BbeI 151 GGCGCC GGCGC^C
BbvCI 1274 CCTCAGC CCTCAGC(-5/-2)
BciVI 598 GTATCC GTATCC(6/5)
BfrI 513 CTTAAG C^TTAAG
BlnI 966 CCTAGG C^CTAGG
BsaHI 151 GGCGCC G[GA]^CG[CT]C
BsmI 859 GAATGC GAATGC(1/-1)
BspLU11I 1723 ACATGT A^CATGT
BsrDI 2523 CATTGC GCAATG(2/0)
BstAPI 432 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2489 TTCGAA TT^CGAA
BstEII 1268 GGTCACC G^GT[ACGT]ACC
BstPI 1268 GGTCACC G^GT[ACGT]ACC
BstYI 2714 AGATCC [GA]^GATC[CT]
Bsu36I 1456 CCTCAGG CC^T[ACGT]AGG
CpoI 1110 CGGACCG CG^G[AT]CCG
DraIII 2653 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 37 CGGCCG C^GGCCG
EclXI 37 CGGCCG C^GGCCG
Eco47III 982 AGCGCT AGC^GCT
Eco52I 37 CGGCCG C^GGCCG
Eco81I 1456 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1268 GGTCACC G^GT[ACGT]ACC
EcoRI 2407 GAATTC G^AATTC
Hin1I 151 GGCGCC G[GA]^CG[CT]C
HincII 2269 GTCAAC GT[CT]^[GA]AC
HindII 2269 GTCAAC GT[CT]^[GA]AC
HindIII 1694 AAGCTT A^AGCTT
KasI 151 GGCGCC G^GCGCC
KpnI 1505 GGTACC GGTAC^C
MflI 2714 AGATCC [GA]^GATC[CT]
MslI 2694 CATCTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 151 GGCGCC GG^CGCC
NruI 1077 TCGCGA TCG^CGA
NspV 2489 TTCGAA TT^CGAA
PflFI 1977 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 2514 CGATCG CGAT^CG
RsrII 1110 CGGACCG CG^G[AT]CCG
ScaI 2213 AGTACT AGT^ACT
SexAI 314 ACCTGGT A^CC[AT]GGT
SfoI 151 GGCGCC GGC^GCC
SfuI 2489 TTCGAA TT^CGAA
SmaI 108 CCCGGG CCC^GGG
StuI 2159 AGGCCT AGG^CCT
TfiI 72 GAATC G^A[AT]TC
Tth111I 1977 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 305 CCACAGGCTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 2714 AGATCC [GA]^GATC[CT]
XmaCI 108 CCCGGG C^CCGGG
XmaI 108 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 28 GTCTAC GT^[AC][GT]AC
1020 GTCTAC
Asp700I 72 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
775 GAACAGCTTC
BbrPI 2140 CACGTG CAC^GTG
2185 CACGTG
BsaAI 2140 CACGTG [CT]AC^GT[GA]
2185 CACGTG
BseRI 59 GAGGAG GAGGAG(10/8)
1957 GAGGAG
BsiEI 37 CGGCCG CG[GA][CT]^CG
2514 CGATCG
Bsp1407I 2136 TGTACA T^GTACA
2339 TGTACA
BspMI 1672 ACCTGC ACCTGC(4/8)
2636 ACCTGC
BsrGI 2136 TGTACA T^GTACA
2339 TGTACA
EarI 1306 CTCTTC CTCTTC(1/4)
2434 CTCTTC
EcoNI 321 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
546 CCTGAGCGAGG
Ksp632I 1306 CTCTTC CTCTTC(1/4)
2434 CTCTTC
MseI 514 TTAA T^TAA
536 TTAA
NcoI 731 CCATGG C^CATGG
2243 CCATGG
PmaCI 2140 CACGTG CAC^GTG
2185 CACGTG
PmlI 2140 CACGTG CAC^GTG
2185 CACGTG
PpuMI 1220 GGGACCT [GA]G^G[AT]CC[CT]
1254 GGGACCC
PstI 169 CTGCAG CTGCA^G
986 CTGCAG
SacI 996 GAGCTC GAGCT^C
1715 GAGCTC
SphI 2321 GCATGC GCATG^C
2527 GCATGC
SspBI 2136 TGTACA T^GTACA
2339 TGTACA
Tru9I 514 TTAA T^TAA
536 TTAA
XmnI 72 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
775 GAACAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AccIII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApaI
ApoI AspHI AvaI AvaII BanI
BanII BbsI BbvI BcgI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaJI BsaWI BseAI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspEI BsrI BssKI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MroI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I