Restriction map for
pF1KE0433
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
Acc65I AccI AccII AccIII AcsI
AflII AflIII AgeI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AvaI
AviII AvrII BalI BamHI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BlnI BlpI
BpmI Bpu1102I BsaBI BsaI BsaWI
BseAI BsiEI BsiWI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraII DrdI EaeI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HhaI HinP1I HincII HindII
HpaI KasI KpnI KspI MamI
MluI MluNI MroI MscI MspA1I
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 972 GACGTC GACGT^C
AclI 1160 AACGTT AA^CGTT
AhdI 823 GACGTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 914 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 823 GACGTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 627 GAGCAC G[AT]GC[AT]^C
AspI 160 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 1007 GGACC G^G[AT]CC
BanI 289 GGCACC G^G[CT][GA]CC
BbsI 319 GTCTTC GAAGAC(2/6)
BcnI 353 CCCGG CC^[GC]GG
BfaI 1225 CTAG C^TAG
BglII 728 AGATCT A^GATCT
BmyI 627 GAGCAC G[AGT]GC[ACT]^C
BpuAI 319 GTCTTC GAAGAC(2/6)
BsaAI 100 TACGTG [CT]AC^GT[GA]
BseRI 250 GAGGAG GAGGAG(10/8)
BsgI 826 GTGCAG GTGCAG(16/14)
BsiHKAI 627 GAGCAC G[AT]GC[AT]^C
BsmBI 970 GAGACG CGTCTC(1/5)
Bsp1286I 627 GAGCAC G[AGT]GC[ACT]^C
BspHI 1112 TCATGA T^CATGA
BsrDI 987 CATTGC GCAATG(2/0)
BsrI 711 CCAGT ACTGG(1/-1)
BstAPI 913 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI 667 TTTAAA TTT^AAA
DraIII 470 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 823 GACGTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 240 GAAGAG CTCTTC(1/4)
HapII 354 CCGG C^CGG
HgaI 209 GCGTC GACGC(5/10)
HindIII 1033 AAGCTT A^AGCTT
HpaII 354 CCGG C^CGG
Ksp632I 240 GAAGAG CTCTTC(1/4)
MaeI 1225 CTAG C^TAG
MspI 354 CCGG C^CGG
NciI 353 CCCGG CC^[GC]GG
NcoI 536 CCATGG C^CATGG
NspI 950 GCATGT [GA]CATG^[CT]
PflFI 160 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 835 GACTC GAGTC(4/5)
Psp1406I 1160 AACGTT AA^CGTT
PstI 179 CTGCAG CTGCA^G
RcaI 1112 TCATGA T^CATGA
SexAI 500 ACCTGGT A^CC[AT]GGT
SfcI 179 CTGCAG C^T[GA][CT]AG
SspI 301 AATATT AAT^ATT
TaqI 752 TCGA T^CGA
TspRI 15 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 160 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 752 TCGA T^CGA
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# TWO CUT ENZYMES ( 16 enzymes) #
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AciI 948 CCGC CCGC(-3/-1)
20 GCGG
AcyI 208 GGCGTC G[GA]^CG[CT]C
972 GACGTC
AlwI 111 GGATC GGATC(4/5)
408 GGATC
BsaHI 208 GGCGTC G[GA]^CG[CT]C
972 GACGTC
BsiYI 439 CCTGGGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
472 CCAAGTGACGG
BslI 439 CCTGGGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
472 CCAAGTGACGG
BsmFI 928 GGGAC GGGAC(10/14)
708 GTCCC
BstYI 111 GGATCT [GA]^GATC[CT]
728 AGATCT
DsaI 536 CCATGG C^C[GA][CT]GG
924 CCACGG
Hin1I 208 GGCGTC G[GA]^CG[CT]C
972 GACGTC
MfeI 563 CAATTG C^AATTG
1097 CAATTG
MflI 111 GGATCT [GA]^GATC[CT]
728 AGATCT
MunI 563 CAATTG C^AATTG
1097 CAATTG
NlaIV 289 GGCACC GG[ACGT]^[ACGT]CC
481 GGAACC
TfiI 59 GAATC G^A[AT]TC
141 GAATC
XhoII 111 GGATCT [GA]^GATC[CT]
728 AGATCT
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# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AfaI AluI BbvI BsaJI BsmAI
BssKI BstF5I BstNI BstXI Cac8I
Cfr13I DdeI DpnII EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeII MaeIII MboI MboII
MnlI MseI MslI MvaI MwoI
NdeII NlaIII RsaI Sau3AI Sau96I
ScrFI SfaNI StyI Tru9I TseI
Tsp45I Tsp509I