Restriction map for
pF1KE0437
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
Alw44I AlwI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BcnI BfrI BglI
BglII BlnI BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstEII BstPI BstUI
BstYI BstZ17I CfoI Cfr10I ClaI
CpoI DraI DraIII EaeI EagI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT14I FauI FseI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HindIII HpaI HpaII
KasI KpnI KspI MamI MfeI
MflI MluI MluNI MroI MscI
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TfiI Tth111I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 4 GTCTAC GT^[AC][GT]AC
AhdI 883 GACTACGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 496 CCTGAGG CC^T[ACGT]AGG
AspEI 883 GACTACGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 378 CTCGGG C^[CT]CG[GA]G
AvaII 890 GGTCC G^G[AT]CC
BbsI 863 GAAGAC GAAGAC(2/6)
BclI 734 TGATCA T^GATCA
BfaI 128 CTAG C^TAG
BpuAI 863 GAAGAC GAAGAC(2/6)
BsaI 709 GAGACC GGTCTC(1/5)
BsgI 572 CTGCAC GTGCAG(16/14)
BsmAI 709 GAGAC GTCTC(1/5)
BsmFI 582 GTCCC GGGAC(10/14)
BsmI 849 GCATTC GAATGC(1/-1)
BsoBI 378 CTCGGG C^[CT]CG[GA]G
BsrDI 798 CATTGC GCAATG(2/0)
BstAPI 151 GCAGACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 282 TTCGAA TT^CGAA
BstXI 552 CCAACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 496 CCTGAGG CC^T[ACGT]AGG
Cac8I 394 GCAGGC GC[ACGT]^[ACGT]GC
Cfr13I 890 GGTCC G^G[ACGT]CC
DraII 889 GGGTCCT [GA]G^G[ACGT]CC[CT]
DrdI 588 GACAGAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 803 CCGTGG C^C[GA][CT]GG
Eam1105I 883 GACTACGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 496 CCTGAGG CC^T[ACGT]AGG
EcoO109I 889 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 755 ATGCAT ATGCA^T
FbaI 734 TGATCA T^GATCA
HincII 330 GTCAAC GT[CT]^[GA]AC
HindII 330 GTCAAC GT[CT]^[GA]AC
MaeI 128 CTAG C^TAG
MaeII 361 ACGT A^CGT
NlaIV 889 GGGTCC GG[ACGT]^[ACGT]CC
NsiI 755 ATGCAT ATGCA^T
NspV 282 TTCGAA TT^CGAA
PflMI 116 CCACCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 889 GGGTCCT [GA]G^G[AT]CC[CT]
PvuII 632 CAGCTG CAG^CTG
Sau96I 890 GGTCC G^G[ACGT]CC
SfuI 282 TTCGAA TT^CGAA
SmlI 452 CTCAAG C^T[CT][GA]AG
TaqI 283 TCGA T^CGA
Tsp509I 286 AATT ^AATT
TthHB8I 283 TCGA T^CGA
Van91I 116 CCACCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 277 CCAGGTTCGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AlwNI 55 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
152 CAGACACTG
BanII 563 GAGCTC G[GA]GC[CT]^C
694 GAGCTC
BlpI 427 GCTGAGC GC^T[ACGT]AGC
634 GCTGAGC
BpmI 458 CTCCAG CTGGAG(16/14)
560 CTGGAG
Bpu1102I 427 GCTGAGC GC^T[ACGT]AGC
634 GCTGAGC
BseRI 262 GAGGAG GAGGAG(10/8)
592 GAGGAG
CelII 427 GCTGAGC GC^T[ACGT]AGC
634 GCTGAGC
DpnII 267 GATC ^GATC
735 GATC
EcoNI 388 CCTGAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
722 CCTTTCCCAGG
MboI 267 GATC ^GATC
735 GATC
MspA1I 422 CCGCTG C[AC]G^C[GT]G
632 CAGCTG
NdeII 267 GATC ^GATC
735 GATC
NspI 624 ACATGC [GA]CATG^[CT]
753 ACATGC
SacI 563 GAGCTC GAGCT^C
694 GAGCTC
Sau3AI 267 GATC ^GATC
735 GATC
Tsp45I 541 GTGAC ^GT[GC]AC
790 GTGAC
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AfaI AluI AspHI BbvI
BmyI BsaJI BsiHKAI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
DdeI EarI EcoRII Fnu4HI FokI
HinfI HphI ItaI Ksp632I MaeIII
MboII MnlI MseI MslI MvaI
MwoI NlaIII PleI RsaI ScrFI
SfaNI Tru9I TseI TspRI