Restriction map for
pF1KE0439
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# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AvaII AviII BalI BamHI
BbvCI BcgI BclI BcnI BfrI
BglI BglII BlpI Bpu1102I BsaBI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrGI BssHII BssSI
Bst1107I BstBI BstUI BstXI BstYI
BstZ17I CelII ClaI CpoI DpnII
DraI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO109I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI KspI MamI MboI
MfeI MflI MluI MluNI MroI
MscI MspA1I MunI MvnI NciI
NdeI NdeII NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsaI RsrII SacII SalI
Sau3AI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tth111I TthHB8I
Van91I XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcsI 271 GAATTT [GA]^AATT[CT]
AcyI 115 GGCGCC G[GA]^CG[CT]C
ApaI 768 GGGCCC GGGCC^C
ApoI 271 GAATTT [GA]^AATT[CT]
Asp700I 897 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 609 CCTAGG C^CTAGG
BanI 115 GGCGCC G^G[CT][GA]CC
BbeI 115 GGCGCC GGCGC^C
BbrPI 85 CACGTG CAC^GTG
BciVI 801 GGATAC GTATCC(6/5)
BlnI 609 CCTAGG C^CTAGG
BpmI 917 CTGGAG CTGGAG(16/14)
BsaAI 85 CACGTG [CT]AC^GT[GA]
BsaHI 115 GGCGCC G[GA]^CG[CT]C
BseRI 39 CTCCTC GAGGAG(10/8)
BspHI 530 TCATGA T^CATGA
BsrDI 123 CATTGC GCAATG(2/0)
BstAPI 18 GCAGTTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 757 GGTTACC G^GT[ACGT]ACC
BstPI 757 GGTTACC G^GT[ACGT]ACC
CfoI 116 GCGC GCG^C
DsaI 764 CCATGG C^C[GA][CT]GG
EaeI 62 CGGCCA [CT]^GGCC[GA]
EarI 388 CTCTTC CTCTTC(1/4)
EcoO65I 757 GGTTACC G^GT[ACGT]ACC
FauI 104 GCGGG CCCGC(4/6)
HaeII 115 GGCGCC [GA]GCGC^[CT]
HgaI 712 GACGC GACGC(5/10)
HhaI 116 GCGC GCG^C
Hin1I 115 GGCGCC G[GA]^CG[CT]C
HinP1I 116 GCGC G^CGC
KasI 115 GGCGCC G^GCGCC
Ksp632I 388 CTCTTC CTCTTC(1/4)
MaeII 86 ACGT A^CGT
NarI 115 GGCGCC GG^CGCC
NcoI 764 CCATGG C^CATGG
PmaCI 85 CACGTG CAC^GTG
PmlI 85 CACGTG CAC^GTG
PstI 137 CTGCAG CTGCA^G
RcaI 530 TCATGA T^CATGA
SacI 385 GAGCTC GAGCT^C
SapI 387 GCTCTTC GCTCTTC(1/4)
SfcI 137 CTGCAG C^T[GA][CT]AG
SfoI 115 GGCGCC GGC^GCC
XbaI 783 TCTAGA T^CTAGA
XmnI 897 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 166 GTAGAC GT^[AC][GT]AC
592 GTAGAC
AlwNI 140 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
521 CAGTGCCTG
AocI 219 CCTTAGG CC^T[ACGT]AGG
256 CCTAAGG
AspHI 291 GAGCAC G[AT]GC[AT]^C
385 GAGCTC
BanII 385 GAGCTC G[GA]GC[CT]^C
768 GGGCCC
BfaI 610 CTAG C^TAG
784 CTAG
BsiHKAI 291 GAGCAC G[AT]GC[AT]^C
385 GAGCTC
Bsu36I 219 CCTTAGG CC^T[ACGT]AGG
256 CCTAAGG
Cfr13I 768 GGGCC G^G[ACGT]CC
769 GGCCC
Eco81I 219 CCTTAGG CC^T[ACGT]AGG
256 CCTAAGG
EcoT14I 609 CCTAGG C^C[AT][AT]GG
764 CCATGG
MaeI 610 CTAG C^TAG
784 CTAG
MseI 551 TTAA T^TAA
877 TTAA
MslI 153 CACAGTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
735 CACTGCTATG
NaeI 118 GCCGGC GCC^GGC
262 GCCGGC
NgoMIV 118 GCCGGC G^CCGGC
262 GCCGGC
Sau96I 768 GGGCC G^G[ACGT]CC
769 GGCCC
StyI 609 CCTAGG C^C[AT][AT]GG
764 CCATGG
Tru9I 551 TTAA T^TAA
877 TTAA
TspRI 521 CAGTG CA[GC]TG[ACGT][ACGT]^
735 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AluI BbsI BbvI BmyI
BpuAI BsaJI BsiYI BslI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
Cac8I Cfr10I DdeI EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeIII MboII MnlI
MspI MvaI MwoI NlaIII NlaIV
ScrFI SfaNI TfiI TseI Tsp45I
Tsp509I