Restriction map for
pF1KE0455
##################################
# NO CUT ENZYMES ( 167 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BcnI
BfaI BfrI BglI BlnI BlpI
BpmI Bpu1102I BpuAI BsaI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspMI BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I Cac8I CelII
ClaI CpoI DdeI DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FseI FspI HaeII
HaeIII HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MfeI
MluI MluNI MscI MunI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccIII 147 TCCGGA T^CCGGA
AcyI 85 GGCGTC G[GA]^CG[CT]C
AlwNI 39 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 462 GGTCC G^G[AT]CC
BanI 70 GGCACC G^G[CT][GA]CC
BclI 98 TGATCA T^GATCA
BglII 323 AGATCT A^GATCT
BmyI 134 GTGCCC G[AGT]GC[ACT]^C
BsaAI 667 TACGTG [CT]AC^GT[GA]
BsaBI 145 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 85 GGCGTC G[GA]^CG[CT]C
BsaWI 147 TCCGGA [AT]^CCGG[AT]
BseAI 147 TCCGGA T^CCGGA
BseRI 561 CTCCTC GAGGAG(10/8)
BsiYI 17 CCTGGACGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 17 CCTGGACGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 134 GTGCCC G[AGT]GC[ACT]^C
BspEI 147 TCCGGA T^CCGGA
BspHI 242 TCATGA T^CATGA
BsrFI 67 ACCGGC [GA]^CCGG[CT]
BsrI 177 ACTGG ACTGG(1/-1)
BstYI 323 AGATCT [GA]^GATC[CT]
Cfr10I 67 ACCGGC [GA]^CCGG[CT]
Cfr13I 462 GGTCC G^G[ACGT]CC
EarI 236 CTCTTC CTCTTC(1/4)
EcoT14I 647 CCAAGG C^C[AT][AT]GG
FbaI 98 TGATCA T^GATCA
Hin1I 85 GGCGTC G[GA]^CG[CT]C
HinfI 447 GAATC G^A[ACGT]TC
HphI 184 GGTGA GGTGA(8/7)
Ksp632I 236 CTCTTC CTCTTC(1/4)
MaeII 668 ACGT A^CGT
MamI 145 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 323 AGATCT [GA]^GATC[CT]
MroI 147 TCCGGA T^CCGGA
MseI 503 TTAA T^TAA
MspA1I 39 CAGCTG C[AC]G^C[GT]G
PvuII 39 CAGCTG CAG^CTG
RcaI 242 TCATGA T^CATGA
Sau96I 462 GGTCC G^G[ACGT]CC
SfcI 423 CTACAG C^T[GA][CT]AG
StyI 647 CCAAGG C^C[AT][AT]GG
TfiI 447 GAATC G^A[AT]TC
Tru9I 503 TTAA T^TAA
Tsp45I 185 GTGAC ^GT[GC]AC
Tsp509I 415 AATT ^AATT
XhoII 323 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AfaI 115 GTAC GT^AC
279 GTAC
AluI 40 AGCT AG^CT
520 AGCT
BspLU11I 212 ACATGT A^CATGT
344 ACATGT
CfoI 13 GCGC GCG^C
290 GCGC
HapII 68 CCGG C^CGG
148 CCGG
HgaI 21 GACGC GACGC(5/10)
86 GCGTC
HhaI 13 GCGC GCG^C
290 GCGC
HinP1I 13 GCGC G^CGC
290 GCGC
HpaII 68 CCGG C^CGG
148 CCGG
MslI 438 CATGTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
597 CACTACGGTG
MspI 68 CCGG C^CGG
148 CCGG
NlaIV 70 GGCACC GG[ACGT]^[ACGT]CC
651 GGAGCC
NspI 212 ACATGT [GA]CATG^[CT]
344 ACATGT
RsaI 115 GTAC GT^AC
279 GTAC
SfaNI 205 GATGC GCATC(5/9)
625 GATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AccII AciI AflIII AlwI BbvI
BsaJI BsrDI BssKI BstF5I BstNI
BstUI DpnII EcoRII Fnu4HI FokI
ItaI MaeIII MboI MboII MnlI
MvaI MvnI MwoI NdeII NlaIII
Sau3AI ScrFI TseI TspRI