##################################
# NO CUT ENZYMES (  183 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AocI        
Aor51HI     ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AvaII       AviII       BalI        BamHI       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BclI        BcnI        BfrI        BglI        BglII       
BpmI        BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsiEI       BsiHKAI     
BsiWI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      CfoI        Cfr10I      ClaI        
CpoI        DpnII       DraI        DraIII      DrdI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HaeII       HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HpaI        HpaII       KasI        KpnI        
KspI        MaeII       MamI        MboI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MseI        MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NdeII       
NgoMIV      NheI        NlaIII      NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshBI       Psp1406I    
PvuI        RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        Sau3AI      SbfI        
ScaI        SexAI       SfaNI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       StuI        
SwaI        TaqI        Tru9I       Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AciI          371  CCGC                 CCGC(-3/-1)
AcsI          135  GAATTT               [GA]^AATT[CT]
AlwNI         356  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          452  GGGCCC               GGGCC^C
ApoI          135  GAATTT               [GA]^AATT[CT]
AvrII         316  CCTAGG               C^CTAGG
BanII         452  GGGCCC               G[GA]GC[CT]^C
BciVI         222  GTATCC               GTATCC(6/5)
BfaI          317  CTAG                 C^TAG
BlnI          316  CCTAGG               C^CTAGG
BmyI          452  GGGCCC               G[AGT]GC[ACT]^C
BsgI          407  CTGCAC               GTGCAG(16/14)
BsmAI          51  GAGAC                GTCTC(1/5)
BsmBI          51  GAGACG               CGTCTC(1/5)
Bsp1286I      452  GGGCCC               G[AGT]GC[ACT]^C
DsaI          270  CCGTGG               C^C[GA][CT]GG
EarI           48  GAAGAG               CTCTTC(1/4)
EcoNI         442  CCTGCTGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       316  CCTAGG               C^C[AT][AT]GG
FauI          370  CCCGC                CCCGC(4/6)
HindIII         4  AAGCTT               A^AGCTT
Ksp632I        48  GAAGAG               CTCTTC(1/4)
MaeI          317  CTAG                 C^TAG
MboII          48  GAAGA                GAAGA(8/7)
MslI          265  CACATCCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PshAI          85  GACGATGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SspI          115  AATATT               AAT^ATT
StyI          316  CCTAGG               C^C[AT][AT]GG
Tsp45I         92  GTCAC                ^GT[GC]AC
Tsp509I       136  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

BseRI         146  CTCCTC               GAGGAG(10/8)
              305  CTCCTC              
BsmFI         175  GTCCC                GGGAC(10/14)
              334  GTCCC               
BsrI          163  CCAGT                ACTGG(1/-1)
              179  CCAGT               
BstAPI        367  GCACCCGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              514  GCACCTGTTGC         
BstEII         91  GGTCACC              G^GT[ACGT]ACC
              212  GGTTACC             
BstPI          91  GGTCACC              G^GT[ACGT]ACC
              212  GGTTACC             
DraII         451  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              452  GGGCCCC             
EcoO109I      451  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              452  GGGCCCC             
EcoO65I        91  GGTCACC              G^GT[ACGT]ACC
              212  GGTTACC             
HaeIII         18  GGCC                 GG^CC
              453  GGCC                
HinfI         231  GATTC                G^A[ACGT]TC
              248  GATTC               
TfiI          231  GATTC                G^A[AT]TC
              248  GATTC               
TspRI         164  CAGTG                CA[GC]TG[ACGT][ACGT]^
              201  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   32 enzymes) #
#########################################

AluI        BanI        BbvI        BlpI        Bpu1102I    
BsaJI       BsiYI       BslI        BssKI       BstF5I      
BstNI       Cac8I       CelII       Cfr13I      DdeI        
EcoRII      Fnu4HI      FokI        HphI        ItaI        
MaeIII      MnlI        MspA1I      MvaI        MwoI        
NlaIV       PstI        PvuII       Sau96I      ScrFI       
SfcI        TseI