Restriction map for
pF1KE0472
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# NO CUT ENZYMES ( 187 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AflIII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DdeI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI HpaII
KasI KpnI KspI MamI MfeI
MflI MluI MluNI MroI MscI
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
StyI SwaI TfiI Tsp509I Tth111I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AlwNI 389 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 169 GAGCAC G[AT]GC[AT]^C
AvaII 381 GGTCC G^G[AT]CC
BclI 269 TGATCA T^GATCA
BfaI 508 CTAG C^TAG
BmyI 169 GAGCAC G[AGT]GC[ACT]^C
BpmI 39 CTGGAG CTGGAG(16/14)
BsiHKAI 169 GAGCAC G[AT]GC[AT]^C
BsmAI 144 GTCTC GTCTC(1/5)
Bsp1286I 169 GAGCAC G[AGT]GC[ACT]^C
BspHI 158 TCATGA T^CATGA
BstF5I 342 CATCC GGATG(2/0)
DpnII 270 GATC ^GATC
DsaI 207 CCGTGG C^C[GA][CT]GG
EarI 67 GAAGAG CTCTTC(1/4)
FbaI 269 TGATCA T^GATCA
FokI 342 CATCC GGATG(9/13)
HaeIII 480 GGCC GG^CC
HindIII 261 AAGCTT A^AGCTT
Ksp632I 67 GAAGAG CTCTTC(1/4)
MaeI 508 CTAG C^TAG
MaeII 11 ACGT A^CGT
MaeIII 13 GTCAC ^GT[ACGT]AC
MboI 270 GATC ^GATC
MseI 53 TTAA T^TAA
MslI 417 CATGAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 485 CAGCTG C[AC]G^C[GT]G
MwoI 283 GCCCCCTTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 270 GATC ^GATC
NheI 507 GCTAGC G^CTAGC
NspI 132 ACATGC [GA]CATG^[CT]
PvuII 485 CAGCTG CAG^CTG
RcaI 158 TCATGA T^CATGA
Sau3AI 270 GATC ^GATC
SspI 82 AATATT AAT^ATT
TaqI 178 TCGA T^CGA
Tru9I 53 TTAA T^TAA
Tsp45I 13 GTCAC ^GT[GC]AC
TspRI 389 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 178 TCGA T^CGA
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# TWO CUT ENZYMES ( 17 enzymes) #
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AfaI 99 GTAC GT^AC
150 GTAC
BbvI 280 GCAGC GCAGC(8/12)
334 GCTGC
BsaJI 207 CCGTGG C^C[ACGT][ACGT]GG
234 CCTGGG
BsgI 126 CTGCAC GTGCAG(16/14)
278 GTGCAG
Cfr13I 381 GGTCC G^G[ACGT]CC
480 GGCCC
Fnu4HI 280 GCAGC GC^[ACGT]GC
334 GCTGC
HinfI 76 GACTC G^A[ACGT]TC
190 GACTC
ItaI 280 GCAGC GC^[ACGT]GC
334 GCTGC
MboII 67 GAAGA GAAGA(8/7)
35 TCTTC
NlaIV 380 GGGTCC GG[ACGT]^[ACGT]CC
480 GGCCCC
PflMI 80 CCAATATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
384 CCAGGCAGTGG
PleI 76 GACTC GAGTC(4/5)
190 GACTC
PstI 194 CTGCAG CTGCA^G
335 CTGCAG
RsaI 99 GTAC GT^AC
150 GTAC
Sau96I 381 GGTCC G^G[ACGT]CC
480 GGCCC
TseI 280 GCAGC G^C[AT]GC
334 GCTGC
Van91I 80 CCAATATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
384 CCAGGCAGTGG
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# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AluI BsiYI BslI BssKI BstNI
Cac8I EcoRII HphI MnlI MvaI
NlaIII ScrFI SfaNI SfcI