Restriction map for
pF1KE0525
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AflIII AgeI AhdI AocI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaII AviII
AvrII BalI BamHI BbeI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BsaBI BsaHI BsgI BsiEI
BsiWI BspDI BspHI BspLU11I BsrFI
BssHII BssSI Bst1107I BstEII BstPI
BstYI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
Hin1I HpaI KasI KpnI Ksp632I
KspI MaeIII MamI MfeI MflI
MluI MluNI MscI MseI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
PacI PflFI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tru9I Tsp45I
Tth111I XbaI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccIII 559 TCCGGA T^CCGGA
AcsI 409 GAATTT [GA]^AATT[CT]
Alw44I 214 GTGCAC G^TGCAC
AlwI 680 GGATC GGATC(4/5)
ApaI 433 GGGCCC GGGCC^C
ApaLI 214 GTGCAC G^TGCAC
ApoI 409 GAATTT [GA]^AATT[CT]
BbrPI 217 CACGTG CAC^GTG
BbsI 148 GAAGAC GAAGAC(2/6)
BcnI 999 CCGGG CC^[GC]GG
BfaI 1012 CTAG C^TAG
BglI 259 GCCCCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1018 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1018 GCTAAGC GC^T[ACGT]AGC
BpuAI 148 GAAGAC GAAGAC(2/6)
BsaAI 217 CACGTG [CT]AC^GT[GA]
BsaI 276 GGTCTC GGTCTC(1/5)
BsaWI 559 TCCGGA [AT]^CCGG[AT]
BseAI 559 TCCGGA T^CCGGA
BseRI 187 GAGGAG GAGGAG(10/8)
BsmBI 198 GAGACG CGTCTC(1/5)
BsmI 31 GAATGC GAATGC(1/-1)
Bsp1407I 731 TGTACA T^GTACA
BspEI 559 TCCGGA T^CCGGA
BsrBI 52 CCGCTC CCGCTC(-3/-3)
BsrGI 731 TGTACA T^GTACA
BsrI 722 ACTGG ACTGG(1/-1)
BstAPI 823 GCAGCCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 130 TTCGAA TT^CGAA
BstXI 921 CCAGGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1018 GCTAAGC GC^T[ACGT]AGC
DraIII 652 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 542 CCGTGG C^C[GA][CT]GG
EaeI 770 CGGCCA [CT]^GGCC[GA]
EcoT14I 400 CCTTGG C^C[AT][AT]GG
HaeII 7 GGCGCT [GA]GCGC^[CT]
MaeI 1012 CTAG C^TAG
MaeII 218 ACGT A^CGT
MroI 559 TCCGGA T^CCGGA
NciI 999 CCGGG CC^[GC]GG
NspV 130 TTCGAA TT^CGAA
PaeR7I 945 CTCGAG C^TCGAG
PflMI 471 CCATGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 217 CACGTG CAC^GTG
PmlI 217 CACGTG CAC^GTG
PstI 160 CTGCAG CTGCA^G
PvuII 193 CAGCTG CAG^CTG
SacI 1102 GAGCTC GAGCT^C
SfaNI 737 GCATC GCATC(5/9)
SfuI 130 TTCGAA TT^CGAA
SmlI 945 CTCGAG C^T[CT][GA]AG
SspBI 731 TGTACA T^GTACA
StyI 400 CCTTGG C^C[AT][AT]GG
TspRI 838 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 471 CCATGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 854 CCATCAATCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 945 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccII 79 CGCG CG^CG
684 CGCG
AvaI 897 CTCGGG C^[CT]CG[GA]G
945 CTCGAG
BanI 707 GGCACC G^G[CT][GA]CC
868 GGCACC
BanII 433 GGGCCC G[GA]GC[CT]^C
1102 GAGCTC
BpmI 141 CTCCAG CTGGAG(16/14)
25 CTGGAG
BsmFI 795 GTCCC GGGAC(10/14)
990 GTCCC
BsoBI 897 CTCGGG C^[CT]CG[GA]G
945 CTCGAG
BsrDI 828 CATTGC GCAATG(2/0)
322 GCAATG
BstUI 79 CGCG CG^CG
684 CGCG
HgaI 293 GACGC GACGC(5/10)
666 GACGC
HincII 334 GTCAAC GT[CT]^[GA]AC
621 GTCAAC
HindII 334 GTCAAC GT[CT]^[GA]AC
621 GTCAAC
MspA1I 182 CAGCGG C[AC]G^C[GT]G
193 CAGCTG
MvnI 79 CGCG CG^CG
684 CGCG
SfcI 160 CTGCAG C^T[GA][CT]AG
1128 CTACAG
TfiI 239 GAATC G^A[AT]TC
446 GATTC
Tsp509I 410 AATT ^AATT
556 AATT
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
BbvI BmyI BsaJI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BspMI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
MboI MboII MnlI MslI MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
TaqI TseI TthHB8I