Restriction map for
pF1KE0548
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# NO CUT ENZYMES ( 181 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BpuAI
BsaAI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII HinfI HpaI KasI
KpnI Ksp632I KspI MaeI MfeI
MluI MroI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI TfiI
Tsp45I Tsp509I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AciI 394 GCGG CCGC(-3/-1)
AluI 488 AGCT AG^CT
BanI 396 GGTGCC G^G[CT][GA]CC
BanII 89 GAGCCC G[GA]GC[CT]^C
BbvI 318 GCAGC GCAGC(8/12)
BcnI 146 CCCGG CC^[GC]GG
BlpI 4 GCTCAGC GC^T[ACGT]AGC
BpmI 45 CTCCAG CTGGAG(16/14)
Bpu1102I 4 GCTCAGC GC^T[ACGT]AGC
BsaBI 457 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 21 GAGGAG GAGGAG(10/8)
BsiYI 55 CCCTCTCAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 55 CCCTCTCAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 232 GAGAC GTCTC(1/5)
BsmFI 425 GGGAC GGGAC(10/14)
BsrI 238 CCAGT ACTGG(1/-1)
BssSI 162 CACGAG CACGAG(-5/-1)
BstXI 401 CCACTGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 408 GCTGGC GC[ACGT]^[ACGT]GC
CelII 4 GCTCAGC GC^T[ACGT]AGC
DrdI 214 GACCGAGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 341 CCAAGG C^C[AT][AT]GG
Fnu4HI 318 GCAGC GC^[ACGT]GC
HapII 147 CCGG C^CGG
HincII 223 GTCAAC GT[CT]^[GA]AC
HindII 223 GTCAAC GT[CT]^[GA]AC
HpaII 147 CCGG C^CGG
HphI 333 GGTGA GGTGA(8/7)
ItaI 318 GCAGC GC^[ACGT]GC
MaeII 428 ACGT A^CGT
MaeIII 130 GTAAC ^GT[ACGT]AC
MamI 457 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 185 TTAA T^TAA
MslI 260 CACAGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 392 CAGCGG C[AC]G^C[GT]G
MspI 147 CCGG C^CGG
MwoI 399 GCCCACTGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 146 CCCGG CC^[GC]GG
NlaIII 414 CATG CATG^
PstI 94 CTGCAG CTGCA^G
SfaNI 449 GCATC GCATC(5/9)
SfcI 94 CTGCAG C^T[GA][CT]AG
StuI 473 AGGCCT AGG^CCT
StyI 341 CCAAGG C^C[AT][AT]GG
Tru9I 185 TTAA T^TAA
TseI 318 GCAGC G^C[AT]GC
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# TWO CUT ENZYMES ( 8 enzymes) #
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AvaII 199 GGACC G^G[AT]CC
271 GGACC
BmyI 89 GAGCCC G[AGT]GC[ACT]^C
397 GTGCCC
Bsp1286I 89 GAGCCC G[AGT]GC[ACT]^C
397 GTGCCC
BstF5I 195 GGATG GGATG(2/0)
456 GGATG
BstYI 167 GGATCT [GA]^GATC[CT]
281 AGATCC
FokI 195 GGATG GGATG(9/13)
456 GGATG
MflI 167 GGATCT [GA]^GATC[CT]
281 AGATCC
XhoII 167 GGATCT [GA]^GATC[CT]
281 AGATCC
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# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
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AlwI BalI BsaJI BssKI BstNI
Cfr13I DdeI DpnII EaeI EcoRII
HaeIII MboI MboII MluNI MnlI
MscI MvaI NdeII NlaIV Sau3AI
Sau96I ScrFI TspRI