Restriction map for
pF1KE0564
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# NO CUT ENZYMES ( 125 enzymes) #
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Acc65I AccI AccII AccIII AcsI
AflII AgeI Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspI AvaI AvrII BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BfaI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaI
BseAI BsiWI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I CelII ClaI
CpoI DraI DrdI EagI EarI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FseI HaeII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MamI MfeI MluI MroI
MunI MvnI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tsp509I Tth111I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AatII 1150 GACGTC GACGT^C
AclI 1078 AACGTT AA^CGTT
AcyI 1150 GACGTC G[GA]^CG[CT]C
AflIII 1181 ACACGT A^C[GA][CT]GT
AocI 993 CCTGAGG CC^T[ACGT]AGG
AviII 605 TGCGCA TGC^GCA
BclI 1070 TGATCA T^GATCA
BsaAI 1182 CACGTA [CT]AC^GT[GA]
BsaHI 1150 GACGTC G[GA]^CG[CT]C
BsaWI 1187 ACCGGA [AT]^CCGG[AT]
BsiEI 312 CGACCG CG[GA][CT]^CG
BsmBI 945 GAGACG CGTCTC(1/5)
BsrBI 1117 GAGCGG CCGCTC(-3/-3)
BsrDI 281 GCAATG GCAATG(2/0)
BsrFI 92 GCCGGC [GA]^CCGG[CT]
BstXI 785 CCAGGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 993 CCTGAGG CC^T[ACGT]AGG
CfoI 606 GCGC GCG^C
Cfr10I 92 GCCGGC [GA]^CCGG[CT]
DraIII 627 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 993 CCTGAGG CC^T[ACGT]AGG
EcoNI 666 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1070 TGATCA T^GATCA
FspI 605 TGCGCA TGC^GCA
HhaI 606 GCGC GCG^C
Hin1I 1150 GACGTC G[GA]^CG[CT]C
HinP1I 606 GCGC G^CGC
HincII 955 GTCAAC GT[CT]^[GA]AC
HindII 955 GTCAAC GT[CT]^[GA]AC
MboII 465 GAAGA GAAGA(8/7)
NaeI 92 GCCGGC GCC^GGC
NgoMIV 92 GCCGGC G^CCGGC
Psp1406I 1078 AACGTT AA^CGTT
PstI 976 CTGCAG CTGCA^G
SacI 454 GAGCTC GAGCT^C
SexAI 1136 ACCAGGT A^CC[AT]GGT
SmlI 43 CTCAAG C^T[CT][GA]AG
StuI 389 AGGCCT AGG^CCT
TfiI 468 GATTC G^A[AT]TC
Tsp45I 19 GTCAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AhdI 424 GACTACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
947 GACGCACGGTC
AlwNI 204 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1138 CAGGTGCTG
ApaI 243 GGGCCC GGGCC^C
1157 GGGCCC
AspEI 424 GACTACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
947 GACGCACGGTC
AspHI 406 GTGCTC G[AT]GC[AT]^C
454 GAGCTC
BamHI 380 GGATCC G^GATCC
611 GGATCC
BsgI 764 CTGCAC GTGCAG(16/14)
1248 CTGCAC
BsiHKAI 406 GTGCTC G[AT]GC[AT]^C
454 GAGCTC
BsmAI 945 GAGAC GTCTC(1/5)
432 GTCTC
BsmI 360 GAATGC GAATGC(1/-1)
489 GAATGC
BstAPI 484 GCAGAGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1310 GCATGCCTTGC
DraII 243 GGGCCCC [GA]G^G[ACGT]CC[CT]
268 GGGGCCT
Eam1105I 424 GACTACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
947 GACGCACGGTC
EcoO109I 243 GGGCCCC [GA]G^G[ACGT]CC[CT]
268 GGGGCCT
FauI 1034 CCCGC CCCGC(4/6)
1280 CCCGC
HgaI 760 GACGC GACGC(5/10)
947 GACGC
MaeIII 19 GTCAC ^GT[ACGT]AC
1226 GTAAC
MslI 750 CATCGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1254 CACCTGTGTG
NspI 133 GCATGC [GA]CATG^[CT]
1310 GCATGC
PflMI 123 CCAGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
885 CCAACAGGTGG
SfcI 976 CTGCAG C^T[GA][CT]AG
1038 CTACAG
SphI 133 GCATGC GCATG^C
1310 GCATGC
Van91I 123 CCAGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
885 CCAACAGGTGG
XcmI 213 CCAGGCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
569 CCATGCGGCAGCTGG
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# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BalI BanII BbvI BcnI BglI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DsaI EaeI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeII
MboI MflI MluNI MnlI MscI
MseI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TaqI Tru9I
TseI TspRI TthHB8I XhoII