Restriction map for
pF1KE0567
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# NO CUT ENZYMES ( 184 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvaII AviII AvrII BalI
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
CelII Cfr13I ClaI CpoI DpnII
DraI DraII DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI HphI KasI KpnI KspI
MaeII MaeIII MamI MboI MfeI
MflI MluI MluNI MroI MscI
MseI MunI MvnI NarI NciI
NcoI NdeI NdeII NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
Sau3AI Sau96I SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
TaqI TfiI Tru9I Tsp45I Tsp509I
Tth111I TthHB8I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 28 enzymes) #
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AfaI 595 GTAC GT^AC
AocI 743 CCTCAGG CC^T[ACGT]AGG
BanI 94 GGCACC G^G[CT][GA]CC
BglI 200 GCCAATCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 27 CTCCAG CTGGAG(16/14)
BsaJI 131 CCCTGG C^C[ACGT][ACGT]GG
BsmFI 693 GTCCC GGGAC(10/14)
BsrI 145 CCAGT ACTGG(1/-1)
BssSI 231 CTCGTG CACGAG(-5/-1)
BstF5I 473 CATCC GGATG(2/0)
Bsu36I 743 CCTCAGG CC^T[ACGT]AGG
EaeI 2 TGGCCG [CT]^GGCC[GA]
Eco81I 743 CCTCAGG CC^T[ACGT]AGG
FauI 719 CCCGC CCCGC(4/6)
FokI 473 CATCC GGATG(9/13)
HinfI 469 GAGTC G^A[ACGT]TC
MslI 141 CACCCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 102 CAGCTG C[AC]G^C[GT]G
NlaIV 94 GGCACC GG[ACGT]^[ACGT]CC
PflMI 41 CCAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 469 GAGTC GAGTC(4/5)
PshAI 129 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 102 CAGCTG CAG^CTG
RsaI 595 GTAC GT^AC
SacI 82 GAGCTC GAGCT^C
StuI 290 AGGCCT AGG^CCT
TspRI 146 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 41 CCAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AspHI 82 GAGCTC G[AT]GC[AT]^C
714 GTGCTC
BanII 82 GAGCTC G[GA]GC[CT]^C
187 GAGCCC
BsiHKAI 82 GAGCTC G[AT]GC[AT]^C
714 GTGCTC
BsiYI 41 CCAACTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
126 CCTGACCCTGG
BslI 41 CCAACTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
126 CCTGACCCTGG
BspMI 460 ACCTGC ACCTGC(4/8)
51 GCAGGT
BsrFI 258 GCCGGC [GA]^CCGG[CT]
507 GCCGGC
CfoI 32 GCGC GCG^C
194 GCGC
Cfr10I 258 GCCGGC [GA]^CCGG[CT]
507 GCCGGC
DdeI 467 CTGAG C^T[ACGT]AG
744 CTCAG
EarI 396 CTCTTC CTCTTC(1/4)
477 CTCTTC
HaeII 31 AGCGCC [GA]GCGC^[CT]
193 AGCGCC
HapII 259 CCGG C^CGG
508 CCGG
HhaI 32 GCGC GCG^C
194 GCGC
HinP1I 32 GCGC G^CGC
194 GCGC
HpaII 259 CCGG C^CGG
508 CCGG
Ksp632I 396 CTCTTC CTCTTC(1/4)
477 CTCTTC
MspI 259 CCGG C^CGG
508 CCGG
NaeI 258 GCCGGC GCC^GGC
507 GCCGGC
NgoMIV 258 GCCGGC G^CCGGC
507 GCCGGC
PstI 672 CTGCAG CTGCA^G
732 CTGCAG
SfcI 672 CTGCAG C^T[GA][CT]AG
732 CTGCAG
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# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AciI AluI AlwNI BbvI BfaI
BmyI BseRI BsgI Bsp1286I BssKI
BstNI Cac8I EcoRII Fnu4HI HaeIII
ItaI MaeI MboII MnlI MvaI
MwoI NlaIII ScrFI TseI